Rozella allomycis (strain CSF55)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Cryptomycota; Cryptomycota incertae sedis; Rozella; Rozella allomycis

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6337 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075AN77|A0A075AN77_ROZAC WD40 repeat-like protein OS=Rozella allomycis (strain CSF55) OX=988480 GN=O9G_000910 PE=4 SV=1
MM1 pKa = 7.51LSSLMGEE8 pKa = 4.23VVISIGEE15 pKa = 4.31STDD18 pKa = 3.49TLSMGDD24 pKa = 3.38LTGISIEE31 pKa = 4.66DD32 pKa = 3.86SLDD35 pKa = 3.48SLIGEE40 pKa = 4.47TLFTGDD46 pKa = 3.57TVVSLMVGLMDD57 pKa = 4.95SLRR60 pKa = 11.84GDD62 pKa = 3.93SLAKK66 pKa = 9.87STTCLAGDD74 pKa = 5.01FEE76 pKa = 5.03TSMTGEE82 pKa = 4.4SLTSLTGDD90 pKa = 4.07SSCCSCLEE98 pKa = 3.9NSMSCLGDD106 pKa = 3.91SISCLKK112 pKa = 11.01DD113 pKa = 3.4SMSCLGDD120 pKa = 4.04SITSSLIVSLLYY132 pKa = 10.82LFSILKK138 pKa = 9.89FALKK142 pKa = 10.75GIGEE146 pKa = 4.74TIDD149 pKa = 3.54SFNDD153 pKa = 3.23FDD155 pKa = 5.35SSTILKK161 pKa = 10.65SNLRR165 pKa = 11.84FF166 pKa = 3.55

Molecular weight: 17.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075AUZ9|A0A075AUZ9_ROZAC Ras-related protein Rab-11A OS=Rozella allomycis (strain CSF55) OX=988480 GN=O9G_003171 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 5.22SFRR5 pKa = 11.84AYY7 pKa = 10.92LKK9 pKa = 10.04GTGMWISDD17 pKa = 3.67YY18 pKa = 10.31ATQRR22 pKa = 11.84NFGIACAVSTALYY35 pKa = 9.64VWFRR39 pKa = 11.84EE40 pKa = 4.21KK41 pKa = 11.31NEE43 pKa = 3.49GRR45 pKa = 11.84LLRR48 pKa = 11.84RR49 pKa = 11.84VARR52 pKa = 11.84VEE54 pKa = 4.03RR55 pKa = 11.84IVRR58 pKa = 11.84RR59 pKa = 11.84LEE61 pKa = 3.69NAVNKK66 pKa = 9.99IGKK69 pKa = 9.09

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6337

0

6337

2471604

41

5289

390.0

44.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.94 ± 0.024

1.543 ± 0.013

5.672 ± 0.02

7.716 ± 0.042

4.973 ± 0.024

4.473 ± 0.024

2.018 ± 0.013

7.392 ± 0.024

8.354 ± 0.033

9.771 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.013

6.137 ± 0.033

3.65 ± 0.021

3.706 ± 0.02

4.36 ± 0.021

7.938 ± 0.033

4.731 ± 0.025

5.913 ± 0.024

0.906 ± 0.01

3.357 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski