Methylocapsa palsarum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; Methylocapsa

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3816 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4AWX5|A0A1I4AWX5_9RHIZ Methanol dehydrogenase [cytochrome c] subunit 2 OS=Methylocapsa palsarum OX=1612308 GN=SAMN05444581_11216 PE=3 SV=1
MM1 pKa = 7.32SAADD5 pKa = 4.42IYY7 pKa = 10.63PCVISSISPEE17 pKa = 4.14DD18 pKa = 3.16GGGYY22 pKa = 7.92VACFPDD28 pKa = 3.56IPGCLGIGEE37 pKa = 4.61TLDD40 pKa = 3.46EE41 pKa = 4.6ALADD45 pKa = 3.67ARR47 pKa = 11.84QALLACLDD55 pKa = 3.56ALKK58 pKa = 10.74AVDD61 pKa = 4.76RR62 pKa = 11.84EE63 pKa = 4.31PPVPSSSPGG72 pKa = 3.13

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4CYM7|A0A1I4CYM7_9RHIZ Uncharacterized protein OS=Methylocapsa palsarum OX=1612308 GN=SAMN05444581_13013 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.14RR3 pKa = 11.84VAEE6 pKa = 3.95ALGVSRR12 pKa = 11.84SNLSEE17 pKa = 4.02KK18 pKa = 10.78ARR20 pKa = 11.84GKK22 pKa = 8.48TPSRR26 pKa = 11.84GPYY29 pKa = 8.83MKK31 pKa = 10.89AEE33 pKa = 4.78DD34 pKa = 4.46DD35 pKa = 3.9ALLPLIRR42 pKa = 11.84GFVDD46 pKa = 3.24EE47 pKa = 5.01RR48 pKa = 11.84PTYY51 pKa = 9.86GYY53 pKa = 10.63RR54 pKa = 11.84RR55 pKa = 11.84IAALVNRR62 pKa = 11.84NRR64 pKa = 11.84AQQGLPPANRR74 pKa = 11.84KK75 pKa = 8.62RR76 pKa = 11.84VHH78 pKa = 7.38RR79 pKa = 11.84IMQRR83 pKa = 11.84QGLLLEE89 pKa = 4.41RR90 pKa = 11.84RR91 pKa = 11.84TARR94 pKa = 11.84RR95 pKa = 11.84QGRR98 pKa = 11.84AHH100 pKa = 7.25DD101 pKa = 3.99GKK103 pKa = 10.92IMVMRR108 pKa = 11.84SNLRR112 pKa = 11.84WCSDD116 pKa = 3.19GLEE119 pKa = 4.05FSCWNGDD126 pKa = 3.83VVRR129 pKa = 11.84MAFIIDD135 pKa = 3.37AFDD138 pKa = 3.92RR139 pKa = 11.84EE140 pKa = 4.5IIAWTAVCGAGISGSDD156 pKa = 3.73VRR158 pKa = 11.84DD159 pKa = 3.49MMLDD163 pKa = 3.2AVEE166 pKa = 4.48SVSGQQ171 pKa = 3.13

Molecular weight: 19.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3816

0

3816

1162880

25

4089

304.7

32.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.127 ± 0.052

0.937 ± 0.012

5.534 ± 0.029

5.629 ± 0.043

3.96 ± 0.025

8.426 ± 0.057

1.971 ± 0.019

5.225 ± 0.027

3.566 ± 0.035

10.148 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.018

2.659 ± 0.024

5.491 ± 0.036

2.997 ± 0.023

7.264 ± 0.044

5.888 ± 0.035

4.801 ± 0.033

6.838 ± 0.032

1.268 ± 0.017

2.164 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski