Haloplanus vescus
Average proteome isoelectric point is 4.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2791 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3VKD8|A0A1H3VKD8_9EURY ABC-2 type transport system ATP-binding protein OS=Haloplanus vescus OX=555874 GN=SAMN04488065_0039 PE=4 SV=1
MM1 pKa = 7.61 TDD3 pKa = 3.11 YY4 pKa = 10.67 NGKK7 pKa = 9.14 VRR9 pKa = 11.84 AVVLAALMVFSVFAGTVALSGTAAANVSSISGQSAQDD46 pKa = 3.39 LVAGQTSVTQEE57 pKa = 3.71 VTYY60 pKa = 9.95 STTIPGDD67 pKa = 3.83 TTPEE71 pKa = 4.36 TYY73 pKa = 10.3 TLDD76 pKa = 3.37 ADD78 pKa = 4.08 AYY80 pKa = 8.92 EE81 pKa = 4.81 GTSSATIQDD90 pKa = 3.4 VSVSLRR96 pKa = 11.84 NADD99 pKa = 4.14 DD100 pKa = 3.88 EE101 pKa = 5.17 LSVGTVSEE109 pKa = 4.22 SSGDD113 pKa = 3.76 YY114 pKa = 11.05 NIDD117 pKa = 3.41 IQDD120 pKa = 3.73 SGTDD124 pKa = 3.52 GTTHH128 pKa = 7.13 SFTLVLTITQDD139 pKa = 3.25 TQGASTGTYY148 pKa = 10.92 DD149 pKa = 3.82 LTLSAASGASSTVSFDD165 pKa = 3.04 ITSLSSNSDD174 pKa = 2.78 KK175 pKa = 11.01 RR176 pKa = 11.84 AGNAAGSGTFDD187 pKa = 3.21 TYY189 pKa = 11.33 DD190 pKa = 3.48 GEE192 pKa = 5.34 GYY194 pKa = 10.94 VFDD197 pKa = 4.77 GANVYY202 pKa = 10.27 QGEE205 pKa = 4.23 SDD207 pKa = 3.57 IEE209 pKa = 4.24 LGGSISGGVVKK220 pKa = 9.29 TAGNDD225 pKa = 3.16 EE226 pKa = 4.34 GVTLEE231 pKa = 4.92 VPDD234 pKa = 4.63 IPQDD238 pKa = 3.56 QATGRR243 pKa = 11.84 YY244 pKa = 5.35 TTNGSNNAPGVTVQRR259 pKa = 11.84 PRR261 pKa = 11.84 VTTLDD266 pKa = 3.27 VNNQYY271 pKa = 10.97 GG272 pKa = 3.28
Molecular weight: 27.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.605
IPC_protein 3.63
Toseland 3.401
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.312
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 4.024
Thurlkill 3.465
EMBOSS 3.605
Sillero 3.757
Patrickios 1.036
IPC_peptide 3.617
IPC2_peptide 3.732
IPC2.peptide.svr19 3.718
Protein with the highest isoelectric point:
>tr|A0A1H3WZD7|A0A1H3WZD7_9EURY Exonuclease III OS=Haloplanus vescus OX=555874 GN=SAMN04488065_1255 PE=4 SV=1
MM1 pKa = 7.52 AAGSVALAAGLDD13 pKa = 3.68 RR14 pKa = 11.84 LVAEE18 pKa = 5.4 PPAALHH24 pKa = 5.41 PVAWLGRR31 pKa = 11.84 ALGPFDD37 pKa = 5.0 RR38 pKa = 11.84 EE39 pKa = 3.81 WRR41 pKa = 11.84 HH42 pKa = 5.6 PRR44 pKa = 11.84 LAGAAVALAVPLFVAAAAAAVVTLGARR71 pKa = 11.84 LDD73 pKa = 3.72 PRR75 pKa = 11.84 IGAVIAGLGCFAATSRR91 pKa = 11.84 RR92 pKa = 11.84 MLLDD96 pKa = 3.09 EE97 pKa = 4.52 ARR99 pKa = 11.84 AVVAASDD106 pKa = 3.89 TDD108 pKa = 4.07 LSAARR113 pKa = 11.84 TRR115 pKa = 11.84 LRR117 pKa = 11.84 ALAGRR122 pKa = 11.84 DD123 pKa = 3.59 ATDD126 pKa = 3.5 LDD128 pKa = 3.99 AGEE131 pKa = 4.28 VRR133 pKa = 11.84 SAAVEE138 pKa = 4.09 SAAEE142 pKa = 3.98 NLSDD146 pKa = 4.7 GLVAPLAGFALFAPISLPAAAGAAAWVKK174 pKa = 10.46 AVNTLDD180 pKa = 3.32 STFGYY185 pKa = 10.3 RR186 pKa = 11.84 SIPMGWAPARR196 pKa = 11.84 LDD198 pKa = 4.94 DD199 pKa = 3.97 IVMWVPARR207 pKa = 11.84 VSAALLAVVAGRR219 pKa = 11.84 PAALRR224 pKa = 11.84 EE225 pKa = 4.02 ARR227 pKa = 11.84 SFARR231 pKa = 11.84 RR232 pKa = 11.84 PASPNAGWPMATLAVVLGVRR252 pKa = 11.84 LTKK255 pKa = 10.57 HH256 pKa = 5.26 GAYY259 pKa = 10.18 DD260 pKa = 3.79 LRR262 pKa = 11.84 PSASHH267 pKa = 5.91 PTPAEE272 pKa = 3.81 AEE274 pKa = 3.93 RR275 pKa = 11.84 GVAVVSRR282 pKa = 11.84 AGWLAFGLTGVVALCC297 pKa = 4.42
Molecular weight: 30.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.165
IPC_protein 10.292
Toseland 10.306
ProMoST 10.233
Dawson 10.438
Bjellqvist 10.277
Wikipedia 10.73
Rodwell 10.306
Grimsley 10.511
Solomon 10.643
Lehninger 10.599
Nozaki 10.35
DTASelect 10.248
Thurlkill 10.335
EMBOSS 10.745
Sillero 10.394
Patrickios 10.058
IPC_peptide 10.643
IPC2_peptide 9.633
IPC2.peptide.svr19 8.513
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2791
0
2791
803080
39
2172
287.7
31.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.121 ± 0.068
0.705 ± 0.015
8.884 ± 0.065
8.047 ± 0.069
3.245 ± 0.032
8.263 ± 0.052
2.021 ± 0.022
3.864 ± 0.034
1.706 ± 0.026
9.066 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.745 ± 0.022
2.235 ± 0.023
4.669 ± 0.032
2.491 ± 0.029
6.789 ± 0.048
5.463 ± 0.041
6.61 ± 0.041
9.305 ± 0.054
1.114 ± 0.017
2.656 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here