Candidatus Methylospira mobilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Candidatus Methylospira

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3961 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q0BJA2|A0A5Q0BJA2_9GAMM 30S ribosomal protein S11 OS=Candidatus Methylospira mobilis OX=1808979 GN=rpsK PE=3 SV=1
MM1 pKa = 7.07TFVVTEE7 pKa = 3.83NCIKK11 pKa = 10.53CKK13 pKa = 10.04YY14 pKa = 7.62TDD16 pKa = 4.08CVDD19 pKa = 3.61VCPVDD24 pKa = 4.48CFHH27 pKa = 7.43EE28 pKa = 5.34GPNFLVIDD36 pKa = 4.26PDD38 pKa = 3.64EE39 pKa = 6.0CIDD42 pKa = 3.79CTLCEE47 pKa = 4.44PEE49 pKa = 4.96CPANAIFSEE58 pKa = 4.6DD59 pKa = 3.54EE60 pKa = 4.0VPAAMEE66 pKa = 5.22DD67 pKa = 4.74FITLNAEE74 pKa = 4.28LAKK77 pKa = 9.53IWPGITEE84 pKa = 4.37VKK86 pKa = 10.39DD87 pKa = 3.65ALPDD91 pKa = 3.76ADD93 pKa = 3.49EE94 pKa = 4.49WKK96 pKa = 10.55GKK98 pKa = 9.59SGKK101 pKa = 8.54TDD103 pKa = 3.46LLEE106 pKa = 4.1RR107 pKa = 4.77

Molecular weight: 11.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q0BL86|A0A5Q0BL86_9GAMM Uncharacterized protein OS=Candidatus Methylospira mobilis OX=1808979 GN=F6R98_19845 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 8.35IKK11 pKa = 10.26RR12 pKa = 11.84VRR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.02TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 8.71KK41 pKa = 10.61LAVV44 pKa = 3.4

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3961

0

3961

1288933

24

9266

325.4

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.33 ± 0.056

1.089 ± 0.015

5.332 ± 0.027

5.99 ± 0.039

3.885 ± 0.025

7.482 ± 0.033

2.354 ± 0.018

5.749 ± 0.028

3.994 ± 0.035

10.854 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.018

3.614 ± 0.033

4.538 ± 0.03

4.082 ± 0.026

6.296 ± 0.036

6.157 ± 0.034

5.029 ± 0.033

6.701 ± 0.032

1.339 ± 0.015

2.829 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski