[Clostridium] asparagiforme DSM 15981

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Enterocloster; Enterocloster asparagiformis

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0D7E8|C0D7E8_9FIRM DUF5720 domain-containing protein OS=[Clostridium] asparagiforme DSM 15981 OX=518636 GN=CLOSTASPAR_05195 PE=4 SV=1
EEE2 pKa = 4.62EE3 pKa = 4.36PAEEE7 pKa = 4.46KK8 pKa = 10.62EE9 pKa = 3.77GEEE12 pKa = 4.18FSEEE16 pKa = 4.55SGIVTRR22 pKa = 11.84DDD24 pKa = 3.38LYYY27 pKa = 11.13DD28 pKa = 3.44KK29 pKa = 10.1TNKKK33 pKa = 9.72FITVQGRR40 pKa = 11.84DDD42 pKa = 2.89STFYYY47 pKa = 10.69IIDDD51 pKa = 3.21YY52 pKa = 8.37DD53 pKa = 3.76PVNEEE58 pKa = 4.83EE59 pKa = 3.84EE60 pKa = 4.32EE61 pKa = 4.4YYY63 pKa = 8.0TYYY66 pKa = 11.01LNQVDDD72 pKa = 4.4EE73 pKa = 4.9DDD75 pKa = 3.65AALLGEEE82 pKa = 4.61EE83 pKa = 4.58EE84 pKa = 4.87AVCDDD89 pKa = 3.9TVKKK93 pKa = 10.72ATGAVNTACPVCASNMAGCAGKKK116 pKa = 9.88AEEE119 pKa = 4.38EEE121 pKa = 4.37KK122 pKa = 10.35VEEE125 pKa = 4.08TAPEEE130 pKa = 4.21EEE132 pKa = 4.11PEEE135 pKa = 4.51KKK137 pKa = 10.45GMGGVLALLLVLALAGGGAFYYY159 pKa = 10.78YY160 pKa = 10.85KKK162 pKa = 10.58IKKK165 pKa = 10.4KKK167 pKa = 9.91KKK169 pKa = 10.36KKK171 pKa = 10.39SADDD175 pKa = 3.94DDD177 pKa = 4.25DD178 pKa = 5.04YY179 pKa = 11.79DD180 pKa = 5.62YY181 pKa = 12.0DDD183 pKa = 4.64DD184 pKa = 4.84EEE186 pKa = 7.0DD187 pKa = 3.84DDD189 pKa = 4.94YY190 pKa = 11.49EE191 pKa = 4.65DD192 pKa = 6.01PPEEE196 pKa = 4.15DD197 pKa = 3.84ASPEEE202 pKa = 4.16GDDD205 pKa = 4.47EEE207 pKa = 4.35EEE209 pKa = 3.97EE210 pKa = 4.44DD211 pKa = 5.37EEE213 pKa = 6.0DD214 pKa = 3.58EE215 pKa = 4.49DD216 pKa = 4.86

Molecular weight: 23.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0CVM3|C0CVM3_9FIRM GroES-like protein OS=[Clostridium] asparagiforme DSM 15981 OX=518636 GN=CLOSTASPAR_01026 PE=4 SV=1
MM1 pKa = 7.51SLLRR5 pKa = 11.84WTVRR9 pKa = 11.84KK10 pKa = 9.51RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ALTSIALSIIFPRR26 pKa = 11.84LFVKK30 pKa = 8.56TWPANSAIFSLKK42 pKa = 9.4TKK44 pKa = 10.53LSGKK48 pKa = 9.74ISPPAHH54 pKa = 5.84GG55 pKa = 4.11

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6817

0

6817

1900233

32

5214

278.7

30.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.687 ± 0.036

1.607 ± 0.013

5.357 ± 0.033

7.119 ± 0.034

4.075 ± 0.022

8.243 ± 0.031

1.668 ± 0.014

6.332 ± 0.032

5.134 ± 0.028

9.356 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.207 ± 0.018

3.708 ± 0.022

3.902 ± 0.02

3.305 ± 0.019

5.632 ± 0.029

5.57 ± 0.023

5.148 ± 0.027

7.077 ± 0.024

1.044 ± 0.011

3.828 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski