Sweet potato leaf curl virus-Spain
Average proteome isoelectric point is 8.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6LI41|I6LI41_9GEMI Capsid protein OS=Sweet potato leaf curl virus-Spain OX=652719 GN=V1 PE=3 SV=1
MM1 pKa = 7.7 LFQSWRR7 pKa = 11.84 PSMAEE12 pKa = 3.68 LWDD15 pKa = 4.09 PLQNPLPDD23 pKa = 3.26 TLYY26 pKa = 11.06 GFRR29 pKa = 11.84 CMLSVKK35 pKa = 9.83 YY36 pKa = 10.41 LQGILKK42 pKa = 9.89 KK43 pKa = 10.45 YY44 pKa = 10.35 EE45 pKa = 4.24 PGTLGFEE52 pKa = 4.51 LCSEE56 pKa = 5.38 LIRR59 pKa = 11.84 IFRR62 pKa = 11.84 VRR64 pKa = 11.84 QYY66 pKa = 11.69 DD67 pKa = 3.54 RR68 pKa = 11.84 ANSRR72 pKa = 11.84 FAEE75 pKa = 4.36 ISSLWGEE82 pKa = 4.2 TGKK85 pKa = 8.6 TEE87 pKa = 4.27 AEE89 pKa = 3.93 LRR91 pKa = 11.84 DD92 pKa = 4.14 SYY94 pKa = 11.36 RR95 pKa = 11.84 ALHH98 pKa = 6.22 WEE100 pKa = 4.38 CCPNCCPKK108 pKa = 10.53 LCPGFKK114 pKa = 9.88 RR115 pKa = 11.84 RR116 pKa = 11.84 PDD118 pKa = 3.47 EE119 pKa = 4.28 EE120 pKa = 4.75 KK121 pKa = 10.81 EE122 pKa = 4.18 GG123 pKa = 3.68
Molecular weight: 14.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.628
IPC2_protein 6.693
IPC_protein 6.81
Toseland 6.338
ProMoST 7.293
Dawson 7.454
Bjellqvist 7.527
Wikipedia 7.366
Rodwell 7.454
Grimsley 6.313
Solomon 7.527
Lehninger 7.556
Nozaki 7.922
DTASelect 7.585
Thurlkill 7.629
EMBOSS 7.688
Sillero 7.966
Patrickios 3.999
IPC_peptide 7.527
IPC2_peptide 7.293
IPC2.peptide.svr19 7.221
Protein with the highest isoelectric point:
>tr|I6LI42|I6LI42_9GEMI Transcriptional activator protein OS=Sweet potato leaf curl virus-Spain OX=652719 GN=C2 PE=3 SV=1
MM1 pKa = 7.54 TGRR4 pKa = 11.84 IPVSRR9 pKa = 11.84 RR10 pKa = 11.84 FHH12 pKa = 6.81 PYY14 pKa = 8.37 GGRR17 pKa = 11.84 PVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 LNFEE26 pKa = 3.93 TAIVPYY32 pKa = 9.22 TGNAVPIAARR42 pKa = 11.84 SYY44 pKa = 8.64 VPVSRR49 pKa = 11.84 GVRR52 pKa = 11.84 MKK54 pKa = 10.51 RR55 pKa = 11.84 KK56 pKa = 9.94 RR57 pKa = 11.84 GDD59 pKa = 4.09 RR60 pKa = 11.84 IPKK63 pKa = 9.95 GCVGPCKK70 pKa = 10.08 VQDD73 pKa = 3.86 YY74 pKa = 8.72 EE75 pKa = 4.55 FKK77 pKa = 10.29 MDD79 pKa = 3.56 VPHH82 pKa = 7.05 TGTFVCVSDD91 pKa = 3.96 FTRR94 pKa = 11.84 GTGLTHH100 pKa = 7.28 RR101 pKa = 11.84 LGKK104 pKa = 9.73 RR105 pKa = 11.84 VCVKK109 pKa = 10.91 SMGIDD114 pKa = 3.27 GKK116 pKa = 11.25 VWMDD120 pKa = 3.91 DD121 pKa = 3.17 NVAKK125 pKa = 9.98 IDD127 pKa = 3.49 HH128 pKa = 6.5 TNIITYY134 pKa = 9.02 WLIRR138 pKa = 11.84 DD139 pKa = 3.65 RR140 pKa = 11.84 RR141 pKa = 11.84 PNKK144 pKa = 10.31 DD145 pKa = 3.09 PLNFVQVFTMYY156 pKa = 11.07 DD157 pKa = 3.63 NEE159 pKa = 4.13 PTTAKK164 pKa = 10.17 IRR166 pKa = 11.84 MDD168 pKa = 3.48 LRR170 pKa = 11.84 DD171 pKa = 3.42 RR172 pKa = 11.84 MQVLKK177 pKa = 10.39 KK178 pKa = 10.29 FSVTVSGGPYY188 pKa = 8.37 SHH190 pKa = 7.5 KK191 pKa = 10.44 EE192 pKa = 3.47 QALVRR197 pKa = 11.84 KK198 pKa = 8.79 FFKK201 pKa = 10.63 GLYY204 pKa = 8.96 NHH206 pKa = 5.46 VTYY209 pKa = 10.87 NHH211 pKa = 6.65 KK212 pKa = 10.86 EE213 pKa = 3.59 EE214 pKa = 4.51 AKK216 pKa = 10.79 YY217 pKa = 10.68 EE218 pKa = 4.05 NHH220 pKa = 7.03 LEE222 pKa = 4.07 NALMLYY228 pKa = 9.96 SASSHH233 pKa = 7.24 DD234 pKa = 4.04 INPVYY239 pKa = 9.39 QTLRR243 pKa = 11.84 CRR245 pKa = 11.84 AYY247 pKa = 10.58 FYY249 pKa = 10.72 DD250 pKa = 3.51 SHH252 pKa = 7.95 NNN254 pKa = 3.3
Molecular weight: 29.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.252
IPC2_protein 9.414
IPC_protein 9.531
Toseland 9.94
ProMoST 9.721
Dawson 10.189
Bjellqvist 9.911
Wikipedia 10.379
Rodwell 10.496
Grimsley 10.277
Solomon 10.218
Lehninger 10.175
Nozaki 9.984
DTASelect 9.897
Thurlkill 10.028
EMBOSS 10.365
Sillero 10.116
Patrickios 9.56
IPC_peptide 10.218
IPC2_peptide 8.756
IPC2.peptide.svr19 8.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
754
85
254
150.8
17.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.642 ± 0.227
3.448 ± 0.903
4.642 ± 0.485
5.172 ± 1.131
4.111 ± 0.596
6.366 ± 0.463
3.05 ± 0.608
5.57 ± 1.082
5.172 ± 0.795
7.692 ± 1.622
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.785 ± 0.543
4.509 ± 0.539
6.233 ± 1.065
3.714 ± 0.801
8.753 ± 0.787
7.162 ± 1.257
6.366 ± 0.614
5.438 ± 1.726
1.592 ± 0.457
3.581 ± 0.718
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here