Spirosoma taeanense

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Spirosoma

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4629 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5YE05|A0A6M5YE05_9BACT NUDIX hydrolase OS=Spirosoma taeanense OX=2735870 GN=HNV11_18325 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84KK3 pKa = 9.98LILYY7 pKa = 8.85IASSLDD13 pKa = 3.32GFIARR18 pKa = 11.84SDD20 pKa = 4.01GSFDD24 pKa = 3.6WLDD27 pKa = 3.38ATPNPNQLDD36 pKa = 3.72YY37 pKa = 10.55GYY39 pKa = 10.6HH40 pKa = 5.48SFYY43 pKa = 11.42VSIDD47 pKa = 3.36TTLMGNSTYY56 pKa = 8.21QTILDD61 pKa = 3.74MGGDD65 pKa = 4.09FPYY68 pKa = 10.17PDD70 pKa = 3.12KK71 pKa = 11.08TNYY74 pKa = 9.46VFSRR78 pKa = 11.84QPGQVDD84 pKa = 3.43TAYY87 pKa = 10.71VRR89 pKa = 11.84YY90 pKa = 8.84ITDD93 pKa = 3.49DD94 pKa = 3.4PAAFVQSLKK103 pKa = 9.87NQEE106 pKa = 4.32GKK108 pKa = 10.69AIWLVGGGQLNAALLNARR126 pKa = 11.84LIDD129 pKa = 4.56EE130 pKa = 5.39IILTLVPTVLGSGIPLFADD149 pKa = 3.52SSLEE153 pKa = 4.19TQFSLTNSEE162 pKa = 4.1SFEE165 pKa = 4.29TGFVQLTYY173 pKa = 11.11APALNSFF180 pKa = 4.24

Molecular weight: 19.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5YF81|A0A6M5YF81_9BACT Tetratricopeptide repeat protein OS=Spirosoma taeanense OX=2735870 GN=HNV11_22655 PE=4 SV=1
MM1 pKa = 6.34VTIRR5 pKa = 11.84NKK7 pKa = 10.14FVLLAAGFWLGGIILLLLGAAFRR30 pKa = 11.84PQSWAGAPLTIGIIGQALGFGFLGFALMQAVFRR63 pKa = 11.84KK64 pKa = 9.87RR65 pKa = 11.84NRR67 pKa = 3.22

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4629

0

4629

1666099

33

3332

359.9

40.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.598 ± 0.036

0.731 ± 0.011

5.35 ± 0.022

5.239 ± 0.033

4.433 ± 0.023

7.347 ± 0.038

1.818 ± 0.019

5.602 ± 0.025

4.653 ± 0.031

10.101 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.015

4.636 ± 0.031

4.749 ± 0.023

4.507 ± 0.026

5.763 ± 0.029

5.971 ± 0.028

6.346 ± 0.036

6.976 ± 0.029

1.307 ± 0.014

3.75 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski