Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium; Mycolicibacterium smegmatis

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0QRL5|A0QRL5_MYCS2 ABC transporter component A OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_1159 PE=4 SV=1
MM1 pKa = 7.52AASQPVLEE9 pKa = 4.82ALGDD13 pKa = 3.6HH14 pKa = 7.52DD15 pKa = 4.3YY16 pKa = 11.66LLRR19 pKa = 11.84FTQDD23 pKa = 1.99YY24 pKa = 8.45DD25 pKa = 3.72APVVVRR31 pKa = 11.84VYY33 pKa = 11.44ADD35 pKa = 3.43PTVVAQIAADD45 pKa = 3.73EE46 pKa = 4.46TRR48 pKa = 11.84VVAATADD55 pKa = 3.7YY56 pKa = 10.93LIARR60 pKa = 11.84QSLEE64 pKa = 4.11DD65 pKa = 3.91LPEE68 pKa = 4.03QVDD71 pKa = 4.05LDD73 pKa = 4.29EE74 pKa = 4.64VAAAYY79 pKa = 10.25DD80 pKa = 4.01DD81 pKa = 4.67YY82 pKa = 12.05VDD84 pKa = 5.33DD85 pKa = 4.12LHH87 pKa = 7.37NRR89 pKa = 11.84LNPPTT94 pKa = 3.87

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O68956|GLMS_MYCS2 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=glmS PE=3 SV=3
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVANRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.67GRR42 pKa = 11.84RR43 pKa = 11.84ALTAA47 pKa = 3.88

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6602

1

6603

2082713

24

7523

315.4

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.946 ± 0.036

0.807 ± 0.01

6.293 ± 0.03

5.392 ± 0.027

3.092 ± 0.017

8.816 ± 0.034

2.274 ± 0.017

4.352 ± 0.022

2.167 ± 0.02

9.79 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.013

2.22 ± 0.015

5.753 ± 0.023

2.927 ± 0.016

7.266 ± 0.029

5.278 ± 0.019

6.162 ± 0.023

8.827 ± 0.029

1.466 ± 0.013

2.125 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski