Amino acid dipepetide frequency for Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.64AlaAla: 19.64 ± 0.134	0.992AlaCys: 0.992 ± 0.023	8.423AlaAsp: 8.423 ± 0.071	8.055AlaGlu: 8.055 ± 0.067	3.532AlaPhe: 3.532 ± 0.044	11.669AlaGly: 11.669 ± 0.085	2.661AlaHis: 2.661 ± 0.037	5.271AlaIle: 5.271 ± 0.053	2.981AlaLys: 2.981 ± 0.047	12.956AlaLeu: 12.956 ± 0.087	2.909AlaMet: 2.909 ± 0.036	2.498AlaAsn: 2.498 ± 0.034	6.355AlaPro: 6.355 ± 0.082	3.995AlaGln: 3.995 ± 0.044	8.518AlaArg: 8.518 ± 0.067	5.881AlaSer: 5.881 ± 0.054	7.233AlaThr: 7.233 ± 0.071	11.954AlaVal: 11.954 ± 0.089	1.574AlaTrp: 1.574 ± 0.029	2.367AlaTyr: 2.367 ± 0.029	0.0AlaXaa: 0.0 ± 0.0
Cys	1.078CysAla: 1.078 ± 0.024	0.096CysCys: 0.096 ± 0.007	0.539CysAsp: 0.539 ± 0.017	0.418CysGlu: 0.418 ± 0.014	0.24CysPhe: 0.24 ± 0.011	0.961CysGly: 0.961 ± 0.024	0.199CysHis: 0.199 ± 0.01	0.261CysIle: 0.261 ± 0.012	0.11CysLys: 0.11 ± 0.007	0.673CysLeu: 0.673 ± 0.017	0.12CysMet: 0.12 ± 0.008	0.151CysAsn: 0.151 ± 0.009	0.477CysPro: 0.477 ± 0.018	0.193CysGln: 0.193 ± 0.009	0.594CysArg: 0.594 ± 0.018	0.48CysSer: 0.48 ± 0.016	0.499CysThr: 0.499 ± 0.015	0.671CysVal: 0.671 ± 0.018	0.128CysTrp: 0.128 ± 0.006	0.183CysTyr: 0.183 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.877AspAla: 7.877 ± 0.072	0.402AspCys: 0.402 ± 0.014	4.397AspAsp: 4.397 ± 0.055	4.159AspGlu: 4.159 ± 0.05	1.792AspPhe: 1.792 ± 0.026	5.854AspGly: 5.854 ± 0.056	1.556AspHis: 1.556 ± 0.029	2.73AspIle: 2.73 ± 0.038	1.273AspLys: 1.273 ± 0.027	6.16AspLeu: 6.16 ± 0.059	1.029AspMet: 1.029 ± 0.025	1.26AspAsn: 1.26 ± 0.025	4.357AspPro: 4.357 ± 0.05	1.752AspGln: 1.752 ± 0.033	4.586AspArg: 4.586 ± 0.054	2.643AspSer: 2.643 ± 0.042	3.391AspThr: 3.391 ± 0.043	5.464AspVal: 5.464 ± 0.057	0.943AspTrp: 0.943 ± 0.024	1.301AspTyr: 1.301 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	6.291GluAla: 6.291 ± 0.065	0.364GluCys: 0.364 ± 0.012	2.596GluAsp: 2.596 ± 0.04	2.568GluGlu: 2.568 ± 0.038	1.925GluPhe: 1.925 ± 0.031	3.378GluGly: 3.378 ± 0.047	1.644GluHis: 1.644 ± 0.03	2.743GluIle: 2.743 ± 0.04	1.452GluLys: 1.452 ± 0.028	6.498GluLeu: 6.498 ± 0.064	1.088GluMet: 1.088 ± 0.024	1.288GluAsn: 1.288 ± 0.027	3.172GluPro: 3.172 ± 0.048	2.28GluGln: 2.28 ± 0.035	4.529GluArg: 4.529 ± 0.05	2.766GluSer: 2.766 ± 0.039	2.909GluThr: 2.909 ± 0.036	4.445GluVal: 4.445 ± 0.045	0.778GluTrp: 0.778 ± 0.019	1.208GluTyr: 1.208 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.078PheAla: 4.078 ± 0.051	0.325PheCys: 0.325 ± 0.013	2.49PheAsp: 2.49 ± 0.034	1.631PheGlu: 1.631 ± 0.028	1.05PhePhe: 1.05 ± 0.025	3.571PheGly: 3.571 ± 0.046	0.648PheHis: 0.648 ± 0.018	1.151PheIle: 1.151 ± 0.027	0.517PheLys: 0.517 ± 0.018	2.638PheLeu: 2.638 ± 0.042	0.518PheMet: 0.518 ± 0.015	0.744PheAsn: 0.744 ± 0.017	1.445PhePro: 1.445 ± 0.026	0.671PheGln: 0.671 ± 0.018	1.681PheArg: 1.681 ± 0.029	1.743PheSer: 1.743 ± 0.032	2.147PheThr: 2.147 ± 0.033	2.698PheVal: 2.698 ± 0.039	0.437PheTrp: 0.437 ± 0.015	0.737PheTyr: 0.737 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	10.17GlyAla: 10.17 ± 0.072	0.784GlyCys: 0.784 ± 0.021	4.95GlyAsp: 4.95 ± 0.053	4.511GlyGlu: 4.511 ± 0.049	3.205GlyPhe: 3.205 ± 0.039	7.629GlyGly: 7.629 ± 0.099	2.098GlyHis: 2.098 ± 0.039	4.29GlyIle: 4.29 ± 0.053	2.279GlyLys: 2.279 ± 0.034	8.671GlyLeu: 8.671 ± 0.081	2.089GlyMet: 2.089 ± 0.03	1.955GlyAsn: 1.955 ± 0.039	4.448GlyPro: 4.448 ± 0.054	2.641GlyGln: 2.641 ± 0.043	6.274GlyArg: 6.274 ± 0.057	5.027GlySer: 5.027 ± 0.047	5.345GlyThr: 5.345 ± 0.055	7.836GlyVal: 7.836 ± 0.061	1.608GlyTrp: 1.608 ± 0.027	2.348GlyTyr: 2.348 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.569HisAla: 2.569 ± 0.039	0.229HisCys: 0.229 ± 0.01	1.441HisAsp: 1.441 ± 0.027	1.124HisGlu: 1.124 ± 0.027	0.71HisPhe: 0.71 ± 0.021	2.155HisGly: 2.155 ± 0.034	0.722HisHis: 0.722 ± 0.02	0.871HisIle: 0.871 ± 0.021	0.375HisLys: 0.375 ± 0.014	2.212HisLeu: 2.212 ± 0.037	0.393HisMet: 0.393 ± 0.014	0.49HisAsn: 0.49 ± 0.015	1.676HisPro: 1.676 ± 0.03	0.657HisGln: 0.657 ± 0.02	1.958HisArg: 1.958 ± 0.03	1.09HisSer: 1.09 ± 0.022	1.339HisThr: 1.339 ± 0.024	1.83HisVal: 1.83 ± 0.033	0.382HisTrp: 0.382 ± 0.016	0.514HisTyr: 0.514 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	6.506IleAla: 6.506 ± 0.067	0.423IleCys: 0.423 ± 0.015	3.381IleAsp: 3.381 ± 0.042	2.672IleGlu: 2.672 ± 0.035	1.124IlePhe: 1.124 ± 0.024	4.701IleGly: 4.701 ± 0.048	0.806IleHis: 0.806 ± 0.021	1.525IleIle: 1.525 ± 0.033	0.877IleLys: 0.877 ± 0.023	3.23IleLeu: 3.23 ± 0.044	0.643IleMet: 0.643 ± 0.019	1.145IleAsn: 1.145 ± 0.026	2.424IlePro: 2.424 ± 0.038	0.894IleGln: 0.894 ± 0.02	2.713IleArg: 2.713 ± 0.031	2.345IleSer: 2.345 ± 0.035	2.894IleThr: 2.894 ± 0.042	3.85IleVal: 3.85 ± 0.048	0.504IleTrp: 0.504 ± 0.018	0.858IleTyr: 0.858 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	2.732LysAla: 2.732 ± 0.041	0.12LysCys: 0.12 ± 0.008	1.088LysAsp: 1.088 ± 0.025	0.973LysGlu: 0.973 ± 0.022	0.676LysPhe: 0.676 ± 0.019	1.531LysGly: 1.531 ± 0.031	0.488LysHis: 0.488 ± 0.016	1.073LysIle: 1.073 ± 0.022	0.667LysLys: 0.667 ± 0.022	2.204LysLeu: 2.204 ± 0.038	0.474LysMet: 0.474 ± 0.016	0.56LysAsn: 0.56 ± 0.017	1.471LysPro: 1.471 ± 0.028	0.684LysGln: 0.684 ± 0.02	1.594LysArg: 1.594 ± 0.032	1.179LysSer: 1.179 ± 0.025	1.412LysThr: 1.412 ± 0.029	1.957LysVal: 1.957 ± 0.033	0.335LysTrp: 0.335 ± 0.012	0.45LysTyr: 0.45 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	13.691LeuAla: 13.691 ± 0.086	0.837LeuCys: 0.837 ± 0.02	6.623LeuAsp: 6.623 ± 0.062	4.454LeuGlu: 4.454 ± 0.053	2.72LeuPhe: 2.72 ± 0.044	8.805LeuGly: 8.805 ± 0.064	2.079LeuHis: 2.079 ± 0.033	4.22LeuIle: 4.22 ± 0.049	1.884LeuLys: 1.884 ± 0.03	9.558LeuLeu: 9.558 ± 0.086	1.82LeuMet: 1.82 ± 0.032	2.084LeuAsn: 2.084 ± 0.034	5.611LeuPro: 5.611 ± 0.059	2.591LeuGln: 2.591 ± 0.04	7.529LeuArg: 7.529 ± 0.07	5.557LeuSer: 5.557 ± 0.055	6.419LeuThr: 6.419 ± 0.06	8.541LeuVal: 8.541 ± 0.062	1.171LeuTrp: 1.171 ± 0.024	1.703LeuTyr: 1.703 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.626MetAla: 2.626 ± 0.04	0.198MetCys: 0.198 ± 0.01	0.875MetAsp: 0.875 ± 0.02	0.721MetGlu: 0.721 ± 0.021	0.671MetPhe: 0.671 ± 0.02	1.484MetGly: 1.484 ± 0.024	0.419MetHis: 0.419 ± 0.013	0.95MetIle: 0.95 ± 0.023	0.47MetLys: 0.47 ± 0.016	2.046MetLeu: 2.046 ± 0.03	0.448MetMet: 0.448 ± 0.016	0.521MetAsn: 0.521 ± 0.017	1.173MetPro: 1.173 ± 0.023	0.532MetGln: 0.532 ± 0.016	1.592MetArg: 1.592 ± 0.029	1.594MetSer: 1.594 ± 0.03	1.892MetThr: 1.892 ± 0.027	1.66MetVal: 1.66 ± 0.027	0.274MetTrp: 0.274 ± 0.011	0.349MetTyr: 0.349 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.631AsnAla: 2.631 ± 0.038	0.189AsnCys: 0.189 ± 0.01	1.196AsnAsp: 1.196 ± 0.025	1.019AsnGlu: 1.019 ± 0.025	0.692AsnPhe: 0.692 ± 0.019	2.115AsnGly: 2.115 ± 0.042	0.492AsnHis: 0.492 ± 0.018	0.985AsnIle: 0.985 ± 0.023	0.472AsnLys: 0.472 ± 0.017	2.137AsnLeu: 2.137 ± 0.034	0.446AsnMet: 0.446 ± 0.013	0.588AsnAsn: 0.588 ± 0.017	1.768AsnPro: 1.768 ± 0.034	0.642AsnGln: 0.642 ± 0.02	1.596AsnArg: 1.596 ± 0.027	1.149AsnSer: 1.149 ± 0.025	1.354AsnThr: 1.354 ± 0.027	1.798AsnVal: 1.798 ± 0.028	0.369AsnTrp: 0.369 ± 0.014	0.56AsnTyr: 0.56 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	7.274ProAla: 7.274 ± 0.085	0.3ProCys: 0.3 ± 0.012	4.401ProAsp: 4.401 ± 0.056	3.961ProGlu: 3.961 ± 0.047	1.624ProPhe: 1.624 ± 0.029	5.526ProGly: 5.526 ± 0.054	1.301ProHis: 1.301 ± 0.025	2.115ProIle: 2.115 ± 0.035	1.268ProLys: 1.268 ± 0.027	4.863ProLeu: 4.863 ± 0.045	1.181ProMet: 1.181 ± 0.021	1.237ProAsn: 1.237 ± 0.025	3.472ProPro: 3.472 ± 0.076	1.892ProGln: 1.892 ± 0.035	3.486ProArg: 3.486 ± 0.044	2.961ProSer: 2.961 ± 0.044	3.418ProThr: 3.418 ± 0.044	5.201ProVal: 5.201 ± 0.05	0.845ProTrp: 0.845 ± 0.02	1.204ProTyr: 1.204 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.606GlnAla: 3.606 ± 0.042	0.231GlnCys: 0.231 ± 0.012	1.183GlnAsp: 1.183 ± 0.025	1.157GlnGlu: 1.157 ± 0.026	0.983GlnPhe: 0.983 ± 0.018	1.921GlnGly: 1.921 ± 0.031	0.733GlnHis: 0.733 ± 0.021	1.601GlnIle: 1.601 ± 0.029	0.665GlnLys: 0.665 ± 0.017	3.296GlnLeu: 3.296 ± 0.041	0.704GlnMet: 0.704 ± 0.018	0.64GlnAsn: 0.64 ± 0.017	1.772GlnPro: 1.772 ± 0.03	1.258GlnGln: 1.258 ± 0.027	2.829GlnArg: 2.829 ± 0.036	1.399GlnSer: 1.399 ± 0.028	1.645GlnThr: 1.645 ± 0.025	2.45GlnVal: 2.45 ± 0.035	0.557GlnTrp: 0.557 ± 0.018	0.639GlnTyr: 0.639 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	8.229ArgAla: 8.229 ± 0.073	0.578ArgCys: 0.578 ± 0.017	4.367ArgAsp: 4.367 ± 0.052	3.997ArgGlu: 3.997 ± 0.046	2.416ArgPhe: 2.416 ± 0.036	5.203ArgGly: 5.203 ± 0.054	1.89ArgHis: 1.89 ± 0.027	3.53ArgIle: 3.53 ± 0.038	1.684ArgLys: 1.684 ± 0.035	7.21ArgLeu: 7.21 ± 0.064	1.827ArgMet: 1.827 ± 0.03	1.744ArgAsn: 1.744 ± 0.026	3.956ArgPro: 3.956 ± 0.049	2.264ArgGln: 2.264 ± 0.033	6.72ArgArg: 6.72 ± 0.071	3.888ArgSer: 3.888 ± 0.047	4.433ArgThr: 4.433 ± 0.048	5.586ArgVal: 5.586 ± 0.048	1.298ArgTrp: 1.298 ± 0.028	1.838ArgTyr: 1.838 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	6.902SerAla: 6.902 ± 0.059	0.392SerCys: 0.392 ± 0.014	3.212SerAsp: 3.212 ± 0.042	2.651SerGlu: 2.651 ± 0.04	1.654SerPhe: 1.654 ± 0.027	5.577SerGly: 5.577 ± 0.056	1.076SerHis: 1.076 ± 0.027	2.178SerIle: 2.178 ± 0.034	1.077SerLys: 1.077 ± 0.023	4.648SerLeu: 4.648 ± 0.051	1.293SerMet: 1.293 ± 0.024	1.094SerAsn: 1.094 ± 0.029	2.993SerPro: 2.993 ± 0.041	1.361SerGln: 1.361 ± 0.024	3.621SerArg: 3.621 ± 0.041	3.044SerSer: 3.044 ± 0.048	3.354SerThr: 3.354 ± 0.037	4.575SerVal: 4.575 ± 0.046	0.885SerTrp: 0.885 ± 0.022	1.187SerTyr: 1.187 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	7.974ThrAla: 7.974 ± 0.066	0.45ThrCys: 0.45 ± 0.015	3.879ThrAsp: 3.879 ± 0.044	3.368ThrGlu: 3.368 ± 0.044	1.889ThrPhe: 1.889 ± 0.031	5.921ThrGly: 5.921 ± 0.056	1.259ThrHis: 1.259 ± 0.025	2.463ThrIle: 2.463 ± 0.035	1.252ThrLys: 1.252 ± 0.027	5.707ThrLeu: 5.707 ± 0.053	1.204ThrMet: 1.204 ± 0.025	1.232ThrAsn: 1.232 ± 0.028	4.055ThrPro: 4.055 ± 0.049	1.508ThrGln: 1.508 ± 0.026	3.783ThrArg: 3.783 ± 0.044	3.272ThrSer: 3.272 ± 0.038	3.984ThrThr: 3.984 ± 0.06	6.208ThrVal: 6.208 ± 0.065	0.853ThrTrp: 0.853 ± 0.02	1.361ThrTyr: 1.361 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	11.652ValAla: 11.652 ± 0.093	0.786ValCys: 0.786 ± 0.018	5.726ValAsp: 5.726 ± 0.056	4.753ValGlu: 4.753 ± 0.056	2.724ValPhe: 2.724 ± 0.039	7.084ValGly: 7.084 ± 0.06	1.84ValHis: 1.84 ± 0.033	4.198ValIle: 4.198 ± 0.05	1.672ValLys: 1.672 ± 0.03	9.179ValLeu: 9.179 ± 0.08	1.667ValMet: 1.667 ± 0.029	2.065ValAsn: 2.065 ± 0.035	4.842ValPro: 4.842 ± 0.048	2.164ValGln: 2.164 ± 0.033	5.998ValArg: 5.998 ± 0.058	4.716ValSer: 4.716 ± 0.049	5.822ValThr: 5.822 ± 0.053	8.714ValVal: 8.714 ± 0.078	1.059ValTrp: 1.059 ± 0.023	1.605ValTyr: 1.605 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.503TrpAla: 1.503 ± 0.029	0.15TrpCys: 0.15 ± 0.009	0.767TrpAsp: 0.767 ± 0.02	0.651TrpGlu: 0.651 ± 0.018	0.519TrpPhe: 0.519 ± 0.016	1.026TrpGly: 1.026 ± 0.024	0.367TrpHis: 0.367 ± 0.013	0.649TrpIle: 0.649 ± 0.016	0.326TrpLys: 0.326 ± 0.013	1.691TrpLeu: 1.691 ± 0.028	0.33TrpMet: 0.33 ± 0.014	0.43TrpAsn: 0.43 ± 0.014	0.816TrpPro: 0.816 ± 0.02	0.619TrpGln: 0.619 ± 0.019	1.217TrpArg: 1.217 ± 0.024	0.922TrpSer: 0.922 ± 0.021	0.89TrpThr: 0.89 ± 0.02	1.142TrpVal: 1.142 ± 0.025	0.31TrpTrp: 0.31 ± 0.012	0.331TrpTyr: 0.331 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.436TyrAla: 2.436 ± 0.031	0.226TyrCys: 0.226 ± 0.01	1.392TyrAsp: 1.392 ± 0.024	1.077TyrGlu: 1.077 ± 0.022	0.773TyrPhe: 0.773 ± 0.022	2.045TyrGly: 2.045 ± 0.033	0.46TyrHis: 0.46 ± 0.015	0.713TyrIle: 0.713 ± 0.018	0.368TyrLys: 0.368 ± 0.013	2.317TyrLeu: 2.317 ± 0.034	0.311TyrMet: 0.311 ± 0.011	0.471TyrAsn: 0.471 ± 0.015	1.245TyrPro: 1.245 ± 0.028	0.669TyrGln: 0.669 ± 0.018	1.84TyrArg: 1.84 ± 0.032	1.12TyrSer: 1.12 ± 0.025	1.24TyrThr: 1.24 ± 0.022	1.689TyrVal: 1.689 ± 0.025	0.348TyrTrp: 0.348 ± 0.013	0.511TyrTyr: 0.511 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6603 proteins (2082713 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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