Trichosporon asahii var. asahii (strain CBS 8904) (Yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Trichosporonales; Trichosporonaceae; Trichosporon; Trichosporon asahii; Trichosporon asahii var. asahii

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8515 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K1VJL2|K1VJL2_TRIAC DNA-(apurinic or apyrimidinic site) endonuclease OS=Trichosporon asahii var. asahii (strain CBS 8904) OX=1220162 GN=A1Q2_06318 PE=3 SV=1
MM1 pKa = 7.14VSYY4 pKa = 10.97YY5 pKa = 10.84DD6 pKa = 3.55EE7 pKa = 5.94LEE9 pKa = 4.99IEE11 pKa = 4.43DD12 pKa = 4.19FTWDD16 pKa = 2.93EE17 pKa = 4.03DD18 pKa = 3.83AKK20 pKa = 10.86VFHH23 pKa = 6.38YY24 pKa = 8.51PCPCGDD30 pKa = 3.24RR31 pKa = 11.84FEE33 pKa = 4.39ISKK36 pKa = 10.37GQLRR40 pKa = 11.84DD41 pKa = 3.33GEE43 pKa = 4.61EE44 pKa = 3.82IATCPSCSLIVRR56 pKa = 11.84VIYY59 pKa = 10.25DD60 pKa = 3.47YY61 pKa = 11.6LDD63 pKa = 3.4WEE65 pKa = 5.15DD66 pKa = 4.45YY67 pKa = 10.63EE68 pKa = 5.49SSDD71 pKa = 3.85EE72 pKa = 4.12EE73 pKa = 4.34EE74 pKa = 3.84EE75 pKa = 4.36AGAEE79 pKa = 4.05EE80 pKa = 5.11AEE82 pKa = 4.81TPATTAPSSPSNVNSVDD99 pKa = 4.17KK100 pKa = 11.27VDD102 pKa = 4.06TPADD106 pKa = 3.44LASALEE112 pKa = 4.18KK113 pKa = 10.95VSVSEE118 pKa = 4.11GDD120 pKa = 3.33RR121 pKa = 11.84DD122 pKa = 3.78SSS124 pKa = 3.63

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K1VYJ6|K1VYJ6_TRIAC Uncharacterized protein OS=Trichosporon asahii var. asahii (strain CBS 8904) OX=1220162 GN=A1Q2_01093 PE=4 SV=1
MM1 pKa = 7.42FPTVARR7 pKa = 11.84SPPQNLGPLLKK18 pKa = 10.24ISVPSSKK25 pKa = 10.37SRR27 pKa = 11.84SPPQNLGPPLKK38 pKa = 10.36ISVPSSKK45 pKa = 10.37SRR47 pKa = 11.84SPPQNLGPLLKK58 pKa = 10.24ISVPSSKK65 pKa = 10.37SRR67 pKa = 11.84SPPQNLGPLLKK78 pKa = 10.24ISVPSSKK85 pKa = 10.37SRR87 pKa = 11.84SPPQNLGPLLKK98 pKa = 10.24ISVPSSKK105 pKa = 10.37SRR107 pKa = 11.84SPPQNLGPLLKK118 pKa = 10.24ISVPSSKK125 pKa = 10.37SRR127 pKa = 11.84SPPQNLGPLLKK138 pKa = 10.24ISVPSSKK145 pKa = 10.37SRR147 pKa = 11.84SPPQNLGPLLKK158 pKa = 10.24ISVPSSKK165 pKa = 10.37SRR167 pKa = 11.84SPPQNLGPLLKK178 pKa = 10.24ISVPSSKK185 pKa = 10.37SRR187 pKa = 11.84SPPQNLGPLLKK198 pKa = 10.24ISVPSSKK205 pKa = 10.37SRR207 pKa = 11.84SPPQNLGPLLKK218 pKa = 10.24ISVPSSKK225 pKa = 10.37SRR227 pKa = 11.84SPPQNLGPLLKK238 pKa = 10.28ISVPPSKK245 pKa = 10.88

Molecular weight: 25.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8515

0

8515

4190985

37

4509

492.2

53.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.077 ± 0.028

1.089 ± 0.008

5.744 ± 0.018

6.271 ± 0.028

3.154 ± 0.017

7.27 ± 0.032

2.358 ± 0.012

3.927 ± 0.019

4.56 ± 0.027

8.607 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.043 ± 0.011

3.11 ± 0.014

7.292 ± 0.038

3.846 ± 0.024

6.602 ± 0.028

8.232 ± 0.037

5.771 ± 0.017

6.264 ± 0.023

1.412 ± 0.01

2.371 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski