Bat polyomavirus 6c
Average proteome isoelectric point is 5.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5ZYM9|A0A0D5ZYM9_9POLY Small t antigen OS=Bat polyomavirus 6c OX=1623688 PE=4 SV=1
MM1 pKa = 7.39 GALLAVIAEE10 pKa = 4.31 VAEE13 pKa = 4.37 VSAVSGLSVDD23 pKa = 3.86 ALLSGEE29 pKa = 4.27 ALASAEE35 pKa = 4.28 LIAGQVEE42 pKa = 4.86 AISALEE48 pKa = 3.89 GLSAVEE54 pKa = 4.08 AAATLGFSAEE64 pKa = 4.18 NYY66 pKa = 9.49 NAIAALASLPNALSGIVGADD86 pKa = 3.11 LVFNGTSVVAAAISTAVSPYY106 pKa = 9.01 TYY108 pKa = 9.78 DD109 pKa = 3.31 YY110 pKa = 11.24 SVPITNLNVNMALQVWQPDD129 pKa = 3.63 LDD131 pKa = 4.09 DD132 pKa = 5.53 LNFPGVMPFVRR143 pKa = 11.84 FLNYY147 pKa = 9.14 IDD149 pKa = 3.9 PMHH152 pKa = 6.68 WAGGLFRR159 pKa = 11.84 ALGRR163 pKa = 11.84 YY164 pKa = 7.6 FWQNAQRR171 pKa = 11.84 QGQQMLEE178 pKa = 4.09 GEE180 pKa = 4.49 FRR182 pKa = 11.84 EE183 pKa = 4.27 LAQRR187 pKa = 11.84 TATSISEE194 pKa = 4.01 TLALYY199 pKa = 10.07 FEE201 pKa = 4.41 NARR204 pKa = 11.84 WAVRR208 pKa = 11.84 AVPTEE213 pKa = 3.81 LYY215 pKa = 10.91 GRR217 pKa = 11.84 LQQYY221 pKa = 8.81 YY222 pKa = 10.89 AEE224 pKa = 5.08 LPPLNPPQLRR234 pKa = 11.84 EE235 pKa = 3.9 VSRR238 pKa = 11.84 RR239 pKa = 11.84 TANQPFQLYY248 pKa = 10.73 DD249 pKa = 4.08 KK250 pKa = 10.14 ILSNVEE256 pKa = 3.58 SAHH259 pKa = 5.88 YY260 pKa = 7.82 VTKK263 pKa = 10.58 VDD265 pKa = 3.97 PPGGANQRR273 pKa = 11.84 QTPDD277 pKa = 2.7 WLLPLILGLYY287 pKa = 10.37 GDD289 pKa = 5.19 ISPSWEE295 pKa = 3.98 TTLEE299 pKa = 3.98 EE300 pKa = 5.04 LEE302 pKa = 4.4 EE303 pKa = 4.52 EE304 pKa = 4.01 EE305 pKa = 5.96 DD306 pKa = 4.01 GPKK309 pKa = 10.07 KK310 pKa = 10.38 KK311 pKa = 10.12 KK312 pKa = 9.91 QRR314 pKa = 11.84 PSTKK318 pKa = 7.82 TTPHH322 pKa = 6.98 RR323 pKa = 11.84 SSKK326 pKa = 8.8 TYY328 pKa = 9.97 SKK330 pKa = 10.44 RR331 pKa = 11.84 RR332 pKa = 11.84 HH333 pKa = 5.04
Molecular weight: 36.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.943
IPC2_protein 5.016
IPC_protein 4.927
Toseland 4.825
ProMoST 4.978
Dawson 4.863
Bjellqvist 5.016
Wikipedia 4.711
Rodwell 4.787
Grimsley 4.736
Solomon 4.863
Lehninger 4.825
Nozaki 4.978
DTASelect 5.092
Thurlkill 4.813
EMBOSS 4.736
Sillero 5.054
Patrickios 3.935
IPC_peptide 4.876
IPC2_peptide 5.054
IPC2.peptide.svr19 5.043
Protein with the highest isoelectric point:
>tr|A0A0D5ZYR4|A0A0D5ZYR4_9POLY Capsid protein VP1 OS=Bat polyomavirus 6c OX=1623688 GN=VP1 PE=3 SV=1
MM1 pKa = 7.66 DD2 pKa = 4.87 HH3 pKa = 7.31 ALTRR7 pKa = 11.84 EE8 pKa = 3.82 EE9 pKa = 4.2 SQRR12 pKa = 11.84 LMEE15 pKa = 4.87 LLNLPMEE22 pKa = 4.43 QFGNFHH28 pKa = 5.99 LMKK31 pKa = 10.33 RR32 pKa = 11.84 AFLSSIKK39 pKa = 10.08 KK40 pKa = 7.26 YY41 pKa = 10.73 HH42 pKa = 6.79 PDD44 pKa = 3.29 KK45 pKa = 11.59 GGDD48 pKa = 3.01 EE49 pKa = 4.46 SMAKK53 pKa = 10.08 EE54 pKa = 4.83 LISLYY59 pKa = 10.58 KK60 pKa = 10.29 KK61 pKa = 10.68 AEE63 pKa = 4.1 CHH65 pKa = 5.45 VSSLEE70 pKa = 4.22 TEE72 pKa = 4.6 DD73 pKa = 3.89 DD74 pKa = 3.76 TSFTTDD80 pKa = 3.05 EE81 pKa = 4.13 VQKK84 pKa = 11.48 ADD86 pKa = 3.27 MFLYY90 pKa = 10.5 LRR92 pKa = 11.84 DD93 pKa = 3.49 WVEE96 pKa = 4.47 CNMGFPCKK104 pKa = 10.22 CLFCMLRR111 pKa = 11.84 KK112 pKa = 9.5 RR113 pKa = 11.84 HH114 pKa = 5.62 NEE116 pKa = 3.55 RR117 pKa = 11.84 KK118 pKa = 9.94 KK119 pKa = 10.82 NLLHH123 pKa = 6.32 NVWGTCYY130 pKa = 10.35 CFKK133 pKa = 10.85 CYY135 pKa = 10.1 ILWFGLEE142 pKa = 4.02 HH143 pKa = 6.21 SWIIFLSWKK152 pKa = 10.33 GIIANTPYY160 pKa = 10.65 RR161 pKa = 11.84 CLDD164 pKa = 3.47 LL165 pKa = 6.4
Molecular weight: 19.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.402
IPC2_protein 6.44
IPC_protein 6.561
Toseland 6.532
ProMoST 7.015
Dawson 7.073
Bjellqvist 7.015
Wikipedia 7.029
Rodwell 7.073
Grimsley 6.605
Solomon 7.102
Lehninger 7.117
Nozaki 7.38
DTASelect 7.366
Thurlkill 7.41
EMBOSS 7.41
Sillero 7.527
Patrickios 3.923
IPC_peptide 7.102
IPC2_peptide 7.088
IPC2.peptide.svr19 6.94
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1532
165
674
383.0
43.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.136 ± 1.7
2.285 ± 0.732
5.091 ± 0.42
7.768 ± 0.727
5.222 ± 0.902
5.091 ± 0.705
2.023 ± 0.432
4.308 ± 0.208
6.593 ± 1.218
10.64 ± 0.77
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.807 ± 0.521
5.222 ± 0.291
5.679 ± 0.962
3.982 ± 0.384
4.504 ± 0.209
6.854 ± 0.287
5.875 ± 0.367
5.679 ± 0.967
1.371 ± 0.314
2.872 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here