Niabella drilacis (strain DSM 25811 / CCM 8410 / LMG 26954 / E90)
Average proteome isoelectric point is 7.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5113 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6TDA1|A0A1G6TDA1_NIADE Uncharacterized protein OS=Niabella drilacis (strain DSM 25811 / CCM 8410 / LMG 26954 / E90) OX=1285928 GN=SAMN04487894_107172 PE=4 SV=1
MM1 pKa = 7.64 GSLKK5 pKa = 10.46 EE6 pKa = 4.02 NPQMVLCRR14 pKa = 11.84 LKK16 pKa = 10.9 EE17 pKa = 4.07 FWADD21 pKa = 3.54 DD22 pKa = 3.37 NTFYY26 pKa = 11.2 LGLVTQYY33 pKa = 11.32 NEE35 pKa = 4.18 AGNEE39 pKa = 3.72 YY40 pKa = 10.67 DD41 pKa = 3.69
Molecular weight: 4.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.948
IPC2_protein 4.317
IPC_protein 4.062
Toseland 3.897
ProMoST 4.037
Dawson 4.037
Bjellqvist 4.317
Wikipedia 3.961
Rodwell 3.91
Grimsley 3.821
Solomon 3.999
Lehninger 3.961
Nozaki 4.177
DTASelect 4.291
Thurlkill 3.961
EMBOSS 3.961
Sillero 4.177
Patrickios 1.952
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.184
Protein with the highest isoelectric point:
>tr|A0A1G6X3S1|A0A1G6X3S1_NIADE TonB-linked outer membrane protein SusC/RagA family OS=Niabella drilacis (strain DSM 25811 / CCM 8410 / LMG 26954 / E90) OX=1285928 GN=SAMN04487894_112125 PE=3 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.32 KK3 pKa = 10.38 FWWFCSLILVMLVTGVFWGTWFTLTRR29 pKa = 11.84 SLEE32 pKa = 4.35 TFPPDD37 pKa = 2.96 HH38 pKa = 7.53 FIRR41 pKa = 11.84 IGQTIIDD48 pKa = 3.92 NVATPMRR55 pKa = 11.84 ILMPATLLLQLLLCISFWKK74 pKa = 9.56 QRR76 pKa = 11.84 PVFYY80 pKa = 10.17 FFAVSFIFMIISLLITLIVEE100 pKa = 4.16 VPIDD104 pKa = 3.53 NQIRR108 pKa = 11.84 TWTAATIPDD117 pKa = 3.55 SWTALRR123 pKa = 11.84 ATWKK127 pKa = 10.05 QFHH130 pKa = 6.86 CFRR133 pKa = 11.84 TLTSILSFVSLAVPVLFRR151 pKa = 11.84 HH152 pKa = 6.59 RR153 pKa = 11.84 SRR155 pKa = 11.84 SAYY158 pKa = 9.77 RR159 pKa = 3.32
Molecular weight: 18.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.619
IPC_protein 10.526
Toseland 10.716
ProMoST 10.891
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.935
Nozaki 10.73
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.867
IPC2.peptide.svr19 8.755
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5113
0
5113
1773835
39
2896
346.9
38.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.217 ± 0.031
0.852 ± 0.011
5.119 ± 0.023
5.379 ± 0.034
4.794 ± 0.024
7.381 ± 0.034
1.884 ± 0.016
6.461 ± 0.025
6.39 ± 0.031
9.499 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.291 ± 0.014
5.125 ± 0.034
4.247 ± 0.023
3.903 ± 0.018
4.839 ± 0.021
5.946 ± 0.025
5.764 ± 0.024
6.321 ± 0.027
1.386 ± 0.015
4.202 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here