Cytophagaceae bacterium BCCC1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; unclassified Cytophagaceae

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257ISG9|A0A257ISG9_9BACT Uncharacterized protein OS=Cytophagaceae bacterium BCCC1 OX=2015573 GN=CFE22_12280 PE=4 SV=1
MM1 pKa = 7.33TSIAFNGFAEE11 pKa = 4.84GTKK14 pKa = 10.01QVSPTDD20 pKa = 3.36QKK22 pKa = 11.26VASLYY27 pKa = 8.88FAPTNAFGSGFNALQRR43 pKa = 11.84NRR45 pKa = 11.84IYY47 pKa = 10.79FHH49 pKa = 6.84IANNATEE56 pKa = 4.0NLYY59 pKa = 10.88FGANLRR65 pKa = 11.84TGGSTSTLTDD75 pKa = 2.87VYY77 pKa = 11.49YY78 pKa = 10.5RR79 pKa = 11.84IKK81 pKa = 10.68DD82 pKa = 3.26AAGNVVSGPTLFTSNITSHH101 pKa = 6.08AQAVAGPSSYY111 pKa = 11.56SPITFNPTSDD121 pKa = 2.9GDD123 pKa = 3.96FYY125 pKa = 11.33IEE127 pKa = 4.2IYY129 pKa = 10.11RR130 pKa = 11.84GNSGGSAQGSTTAFVAPWFDD150 pKa = 3.73FSVGTSTTAITDD162 pKa = 3.13GRR164 pKa = 11.84VFCEE168 pKa = 3.51NWSFRR173 pKa = 11.84ATKK176 pKa = 10.21PSDD179 pKa = 3.29NFTGTFTDD187 pKa = 4.14PVSPTLYY194 pKa = 10.48SYY196 pKa = 11.4TNDD199 pKa = 3.17EE200 pKa = 4.36TVVKK204 pKa = 11.04VEE206 pKa = 4.62FNDD209 pKa = 4.02FQPLSFIPAFNSYY222 pKa = 10.42GVDD225 pKa = 3.44PLQTDD230 pKa = 3.33WSIGRR235 pKa = 11.84KK236 pKa = 9.28SINSNVSAPVLSGGYY251 pKa = 8.18RR252 pKa = 11.84TFLNEE257 pKa = 3.86PDD259 pKa = 3.35ASVYY263 pKa = 10.47PSASLAAAPTLNGNVTGCAGAYY285 pKa = 7.53FIPYY289 pKa = 7.2FTNVDD294 pKa = 2.84GDD296 pKa = 3.82VRR298 pKa = 11.84FLLDD302 pKa = 3.21INGTAGFQLGTTDD315 pKa = 5.19RR316 pKa = 11.84ILEE319 pKa = 4.26AFGVSAGSHH328 pKa = 5.66LMPWDD333 pKa = 3.93GLDD336 pKa = 3.3GLGNVLSPNTTVTSQISMLRR356 pKa = 11.84GRR358 pKa = 11.84INLPMYY364 pKa = 9.85DD365 pKa = 3.68AEE367 pKa = 4.32MNRR370 pKa = 11.84NGFSLTAVSPVLVSNLRR387 pKa = 11.84MYY389 pKa = 10.41WDD391 pKa = 4.4DD392 pKa = 4.56ASLTNHH398 pKa = 6.72ANNTTGAGVDD408 pKa = 3.72NSALGQVSPGHH419 pKa = 5.98GWNGTYY425 pKa = 10.68GSTLLVPPVLPGGEE439 pKa = 4.44GSDD442 pKa = 3.84TPSDD446 pKa = 3.85ANDD449 pKa = 3.5DD450 pKa = 4.03FGNLRR455 pKa = 11.84TINTWFWGLEE465 pKa = 4.15EE466 pKa = 4.85QSGTLNFRR474 pKa = 11.84LPYY477 pKa = 10.0CISIQGNVYY486 pKa = 10.82SDD488 pKa = 4.14ANGLNNSLINGTGSNFGGLYY508 pKa = 10.2AYY510 pKa = 10.34LVNTLGNVVAIDD522 pKa = 3.88TVNSDD527 pKa = 2.66GTYY530 pKa = 9.98IFNNIDD536 pKa = 3.31SGSFTVRR543 pKa = 11.84ISTNTGVVGNPAPVIAFPTGYY564 pKa = 8.46STIGEE569 pKa = 4.21GTAASGDD576 pKa = 3.83GTPSGDD582 pKa = 3.45TPITVVASNITGVNFGLNQLPNSNTASGTFLNPTGTNTVTVPTLGGSDD630 pKa = 4.32PEE632 pKa = 5.37DD633 pKa = 3.97GSLGVTNSIIIKK645 pKa = 8.95VLPLNGTLYY654 pKa = 10.26YY655 pKa = 10.85NGIAVVDD662 pKa = 4.05EE663 pKa = 4.08QVIASYY669 pKa = 11.34NSALLTLDD677 pKa = 3.54PSFPSVGTVVFDD689 pKa = 3.7YY690 pKa = 11.53AFFDD694 pKa = 3.5NTGLVDD700 pKa = 3.76PTPATVAINFTNNPPVANNDD720 pKa = 3.11TNAPIASTAGQTAINALTATDD741 pKa = 3.71TDD743 pKa = 4.04GTIANYY749 pKa = 10.2RR750 pKa = 11.84IEE752 pKa = 4.19TLPTLGTLYY761 pKa = 10.76VGGVAATVGQVLTPAQVATLTYY783 pKa = 10.41DD784 pKa = 3.39PSGTANGTDD793 pKa = 3.11SFTFTAIDD801 pKa = 3.41NNGAEE806 pKa = 4.73DD807 pKa = 3.71ATDD810 pKa = 3.42ATVSIPVTNNPPVANNDD827 pKa = 3.11TNAPIASTAGQTAINALTATDD848 pKa = 3.71TDD850 pKa = 4.04GTIANYY856 pKa = 10.15RR857 pKa = 11.84IEE859 pKa = 4.25SLPTLGTLYY868 pKa = 10.76VGGVAATVGQVLTPAQVATLTYY890 pKa = 10.41DD891 pKa = 3.39PSGTANGTDD900 pKa = 3.11SFTFTAIDD908 pKa = 3.41NNGAEE913 pKa = 4.73DD914 pKa = 3.73ATDD917 pKa = 3.46ATISIPVTNNPPVANNNTNAPIASTAGQTAINALTATDD955 pKa = 3.71TDD957 pKa = 4.04GTIANYY963 pKa = 10.15RR964 pKa = 11.84IEE966 pKa = 4.46SLPTMGTLYY975 pKa = 10.76VGGVAATVGQVLTPAQVATLTYY997 pKa = 10.41DD998 pKa = 3.39PSGTANGTDD1007 pKa = 3.11SFTFTAIDD1015 pKa = 3.41NNGAEE1020 pKa = 4.73DD1021 pKa = 3.71ATDD1024 pKa = 3.42ATVSIPVNNNPPVANNNTNAPIASTAGQTAINALTATDD1062 pKa = 3.71TDD1064 pKa = 4.04GTIANYY1070 pKa = 10.15RR1071 pKa = 11.84IEE1073 pKa = 4.46SLPTMGTLYY1082 pKa = 10.76VGGVAATVGQVLTPAQVATLTYY1104 pKa = 10.73DD1105 pKa = 3.47PAGTANGTDD1114 pKa = 3.23SFTFTAIDD1122 pKa = 3.41NNGAEE1127 pKa = 4.73DD1128 pKa = 3.73ATDD1131 pKa = 3.46ATISIPVNNIPPIALDD1147 pKa = 5.64DD1148 pKa = 4.12SDD1150 pKa = 5.41LYY1152 pKa = 11.6NAFGTNGIVNILANDD1167 pKa = 3.95TLSTGILATTLNANIDD1183 pKa = 4.25LNPATPALDD1192 pKa = 3.22NTLIVGGEE1200 pKa = 3.88GTYY1203 pKa = 9.92TYY1205 pKa = 10.9NLSNGQVTFVPEE1217 pKa = 3.69IGLTGNPAPINYY1229 pKa = 9.33VLIDD1233 pKa = 3.79NNTGQKK1239 pKa = 8.76DD1240 pKa = 3.39TASIVITYY1248 pKa = 10.33SGFPNAVNDD1257 pKa = 3.7EE1258 pKa = 4.45DD1259 pKa = 5.48LNNLPGIVGVVNALSNDD1276 pKa = 3.76TLSTGAIANTTNASIDD1292 pKa = 4.26LNPLTPTVDD1301 pKa = 3.83HH1302 pKa = 7.09ILTISGQGTYY1312 pKa = 9.93QYY1314 pKa = 11.33NLTTGIVTFIPDD1326 pKa = 3.39NGLIGNPTPINYY1338 pKa = 9.6NLIEE1342 pKa = 4.25LTSGFTDD1349 pKa = 3.24MATISMTYY1357 pKa = 9.82LLPVVAVNDD1366 pKa = 4.3TNTSSPVGVNVTFDD1380 pKa = 3.14IVSNDD1385 pKa = 3.33SLSNGAIATPAEE1397 pKa = 4.12VLVDD1401 pKa = 3.84INLTTPGYY1409 pKa = 9.47QDD1411 pKa = 3.37SLIVSGEE1418 pKa = 4.34GIWSYY1423 pKa = 11.84NSTTGEE1429 pKa = 3.78ITFDD1433 pKa = 3.7PEE1435 pKa = 3.96TGFVNDD1441 pKa = 4.65PTPISYY1447 pKa = 10.24IITEE1451 pKa = 4.28AMTGLLDD1458 pKa = 3.58TATISIMYY1466 pKa = 7.78GVPPIANSDD1475 pKa = 3.37ADD1477 pKa = 5.7LINQPQTSTVINLLANDD1494 pKa = 4.63LLSNLSTATFSMVDD1508 pKa = 3.18VDD1510 pKa = 5.92LEE1512 pKa = 4.2PTALGIQTSVLVPGEE1527 pKa = 3.88GNYY1530 pKa = 10.1VYY1532 pKa = 10.93NSSNGNLTFTPSIGNYY1548 pKa = 10.09SNPTPLIYY1556 pKa = 10.66SLIEE1560 pKa = 4.03KK1561 pKa = 9.09ATGLSDD1567 pKa = 3.43TARR1570 pKa = 11.84VTIQYY1575 pKa = 9.37QFSPSLEE1582 pKa = 4.29LIKK1585 pKa = 10.29TNTLMGSGLVGDD1597 pKa = 4.41SVQYY1601 pKa = 11.14DD1602 pKa = 3.18FTVINTGNVPVTGITITDD1620 pKa = 3.69TKK1622 pKa = 10.51ISSTPIPVTPSALAPGGTAVASRR1645 pKa = 11.84MYY1647 pKa = 10.56YY1648 pKa = 8.99LTAGDD1653 pKa = 3.65ILAGEE1658 pKa = 4.61VLNSALVNGTSSNAIAVKK1676 pKa = 9.36DD1677 pKa = 3.69TSDD1680 pKa = 3.42NGDD1683 pKa = 3.29PGMPGRR1689 pKa = 11.84SNPTRR1694 pKa = 11.84LLLPLSSDD1702 pKa = 3.07VDD1704 pKa = 3.8VKK1706 pKa = 10.86LTGTIIGNCEE1716 pKa = 3.86RR1717 pKa = 11.84VVGDD1721 pKa = 3.37IVTYY1725 pKa = 6.92QVKK1728 pKa = 9.27VFRR1731 pKa = 11.84EE1732 pKa = 3.95DD1733 pKa = 3.33SNAGLVDD1740 pKa = 3.7VVVKK1744 pKa = 10.87DD1745 pKa = 3.54SLGVNFEE1752 pKa = 4.54LVSQIATEE1760 pKa = 4.38GSFNIGTSRR1769 pKa = 11.84WSGISLASGDD1779 pKa = 3.87TATLTFQLRR1788 pKa = 11.84ILTNIGGLTCVEE1800 pKa = 4.28SWVDD1804 pKa = 3.36SLSRR1808 pKa = 11.84ADD1810 pKa = 4.57LDD1812 pKa = 4.28SNPGNRR1818 pKa = 11.84ITTEE1822 pKa = 3.68DD1823 pKa = 4.48DD1824 pKa = 2.69IARR1827 pKa = 11.84ACVSVPISICTTKK1840 pKa = 11.07AEE1842 pKa = 4.17TAEE1845 pKa = 4.38LSTSLGYY1852 pKa = 10.28SSYY1855 pKa = 9.28QWYY1858 pKa = 10.48RR1859 pKa = 11.84NGTLISGAISPTFTATQGGSYY1880 pKa = 8.86TVIVNGSLCPNGSCCPIVVQEE1901 pKa = 4.18NCPCPVEE1908 pKa = 3.96ICLPIAIRR1916 pKa = 11.84KK1917 pKa = 6.22TRR1919 pKa = 3.2

Molecular weight: 199.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257J1M5|A0A257J1M5_9BACT PnuC protein OS=Cytophagaceae bacterium BCCC1 OX=2015573 GN=CFE22_00745 PE=4 SV=1
MM1 pKa = 7.41AQLCSRR7 pKa = 11.84GRR9 pKa = 11.84YY10 pKa = 8.42FDD12 pKa = 3.61KK13 pKa = 11.02KK14 pKa = 10.74SFFLGFVRR22 pKa = 11.84HH23 pKa = 5.87PRR25 pKa = 11.84FNGGVYY31 pKa = 9.53TYY33 pKa = 10.77TSFFEE38 pKa = 4.25KK39 pKa = 10.25TSVNTLRR46 pKa = 11.84RR47 pKa = 11.84HH48 pKa = 4.76WNGVNTGVNRR58 pKa = 11.84GVNILYY64 pKa = 9.43TGAA67 pKa = 3.96

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3514

0

3514

1214332

30

6045

345.6

38.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.447 ± 0.044

0.839 ± 0.019

5.116 ± 0.028

6.28 ± 0.057

5.578 ± 0.044

6.808 ± 0.045

1.633 ± 0.019

7.848 ± 0.044

8.012 ± 0.065

9.311 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.024

6.151 ± 0.043

3.622 ± 0.026

3.282 ± 0.024

3.29 ± 0.026

6.811 ± 0.048

5.576 ± 0.084

6.106 ± 0.037

1.162 ± 0.015

3.832 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski