Amino acid dipepetide frequency for Cytophagaceae bacterium BCCC1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.629AlaAla: 4.629 ± 0.092
0.634AlaCys: 0.634 ± 0.025
3.469AlaAsp: 3.469 ± 0.057
4.218AlaGlu: 4.218 ± 0.073
3.56AlaPhe: 3.56 ± 0.064
4.516AlaGly: 4.516 ± 0.075
1.004AlaHis: 1.004 ± 0.035
5.181AlaIle: 5.181 ± 0.085
4.822AlaLys: 4.822 ± 0.077
5.834AlaLeu: 5.834 ± 0.087
1.579AlaMet: 1.579 ± 0.044
3.679AlaAsn: 3.679 ± 0.064
2.042AlaPro: 2.042 ± 0.064
2.453AlaGln: 2.453 ± 0.051
1.962AlaArg: 1.962 ± 0.043
4.238AlaSer: 4.238 ± 0.087
3.757AlaThr: 3.757 ± 0.138
3.854AlaVal: 3.854 ± 0.062
0.667AlaTrp: 0.667 ± 0.023
2.373AlaTyr: 2.373 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.441CysAla: 0.441 ± 0.023
0.117CysCys: 0.117 ± 0.011
0.416CysAsp: 0.416 ± 0.023
0.507CysGlu: 0.507 ± 0.026
0.483CysPhe: 0.483 ± 0.018
0.799CysGly: 0.799 ± 0.056
0.183CysHis: 0.183 ± 0.014
0.608CysIle: 0.608 ± 0.023
0.541CysLys: 0.541 ± 0.024
0.687CysLeu: 0.687 ± 0.025
0.169CysMet: 0.169 ± 0.011
0.449CysAsn: 0.449 ± 0.026
0.386CysPro: 0.386 ± 0.025
0.338CysGln: 0.338 ± 0.024
0.258CysArg: 0.258 ± 0.015
0.623CysSer: 0.623 ± 0.027
0.471CysThr: 0.471 ± 0.03
0.53CysVal: 0.53 ± 0.026
0.094CysTrp: 0.094 ± 0.01
0.296CysTyr: 0.296 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.429AspAla: 3.429 ± 0.058
0.407AspCys: 0.407 ± 0.021
2.228AspAsp: 2.228 ± 0.045
3.245AspGlu: 3.245 ± 0.051
3.73AspPhe: 3.73 ± 0.077
3.606AspGly: 3.606 ± 0.068
0.832AspHis: 0.832 ± 0.031
4.112AspIle: 4.112 ± 0.063
4.013AspLys: 4.013 ± 0.073
5.273AspLeu: 5.273 ± 0.078
1.098AspMet: 1.098 ± 0.032
2.643AspAsn: 2.643 ± 0.05
1.813AspPro: 1.813 ± 0.038
1.539AspGln: 1.539 ± 0.039
1.707AspArg: 1.707 ± 0.041
3.066AspSer: 3.066 ± 0.056
2.325AspThr: 2.325 ± 0.05
2.877AspVal: 2.877 ± 0.053
0.82AspTrp: 0.82 ± 0.031
2.402AspTyr: 2.402 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
3.982GluAla: 3.982 ± 0.072
0.371GluCys: 0.371 ± 0.017
2.954GluAsp: 2.954 ± 0.052
4.171GluGlu: 4.171 ± 0.104
2.929GluPhe: 2.929 ± 0.048
3.64GluGly: 3.64 ± 0.068
0.996GluHis: 0.996 ± 0.032
5.989GluIle: 5.989 ± 0.087
6.319GluLys: 6.319 ± 0.089
5.611GluLeu: 5.611 ± 0.081
1.663GluMet: 1.663 ± 0.045
4.81GluAsn: 4.81 ± 0.081
1.631GluPro: 1.631 ± 0.037
1.844GluGln: 1.844 ± 0.042
2.202GluArg: 2.202 ± 0.05
3.405GluSer: 3.405 ± 0.06
3.343GluThr: 3.343 ± 0.049
4.037GluVal: 4.037 ± 0.061
0.67GluTrp: 0.67 ± 0.027
2.232GluTyr: 2.232 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.277PheAla: 3.277 ± 0.049
0.506PheCys: 0.506 ± 0.021
3.394PheAsp: 3.394 ± 0.056
3.975PheGlu: 3.975 ± 0.065
3.093PhePhe: 3.093 ± 0.069
4.299PheGly: 4.299 ± 0.068
0.848PheHis: 0.848 ± 0.032
3.695PheIle: 3.695 ± 0.063
3.764PheLys: 3.764 ± 0.062
5.499PheLeu: 5.499 ± 0.098
1.217PheMet: 1.217 ± 0.036
3.039PheAsn: 3.039 ± 0.055
1.843PhePro: 1.843 ± 0.037
1.723PheGln: 1.723 ± 0.038
1.811PheArg: 1.811 ± 0.043
4.445PheSer: 4.445 ± 0.07
2.98PheThr: 2.98 ± 0.053
3.482PheVal: 3.482 ± 0.067
0.694PheTrp: 0.694 ± 0.025
2.193PheTyr: 2.193 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
4.034GlyAla: 4.034 ± 0.073
0.716GlyCys: 0.716 ± 0.046
3.141GlyAsp: 3.141 ± 0.067
3.56GlyGlu: 3.56 ± 0.057
4.112GlyPhe: 4.112 ± 0.066
5.187GlyGly: 5.187 ± 0.09
1.131GlyHis: 1.131 ± 0.037
5.712GlyIle: 5.712 ± 0.073
5.587GlyLys: 5.587 ± 0.082
6.388GlyLeu: 6.388 ± 0.087
1.675GlyMet: 1.675 ± 0.04
3.897GlyAsn: 3.897 ± 0.076
1.598GlyPro: 1.598 ± 0.047
2.298GlyGln: 2.298 ± 0.043
2.316GlyArg: 2.316 ± 0.057
4.379GlySer: 4.379 ± 0.077
4.271GlyThr: 4.271 ± 0.173
4.453GlyVal: 4.453 ± 0.076
0.878GlyTrp: 0.878 ± 0.025
2.748GlyTyr: 2.748 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.012HisAla: 1.012 ± 0.029
0.172HisCys: 0.172 ± 0.011
0.724HisAsp: 0.724 ± 0.023
0.949HisGlu: 0.949 ± 0.029
1.116HisPhe: 1.116 ± 0.031
1.076HisGly: 1.076 ± 0.036
0.451HisHis: 0.451 ± 0.024
1.147HisIle: 1.147 ± 0.028
1.048HisLys: 1.048 ± 0.032
1.701HisLeu: 1.701 ± 0.039
0.274HisMet: 0.274 ± 0.016
0.85HisAsn: 0.85 ± 0.028
0.856HisPro: 0.856 ± 0.029
0.705HisGln: 0.705 ± 0.026
0.611HisArg: 0.611 ± 0.022
1.013HisSer: 1.013 ± 0.027
0.847HisThr: 0.847 ± 0.032
0.863HisVal: 0.863 ± 0.029
0.222HisTrp: 0.222 ± 0.015
0.689HisTyr: 0.689 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.074IleAla: 5.074 ± 0.083
0.83IleCys: 0.83 ± 0.035
4.483IleAsp: 4.483 ± 0.067
5.287IleGlu: 5.287 ± 0.073
4.201IlePhe: 4.201 ± 0.069
5.363IleGly: 5.363 ± 0.077
1.248IleHis: 1.248 ± 0.033
5.598IleIle: 5.598 ± 0.09
6.039IleLys: 6.039 ± 0.081
7.528IleLeu: 7.528 ± 0.099
1.447IleMet: 1.447 ± 0.041
4.65IleAsn: 4.65 ± 0.069
3.167IlePro: 3.167 ± 0.049
2.448IleGln: 2.448 ± 0.055
2.533IleArg: 2.533 ± 0.047
6.177IleSer: 6.177 ± 0.081
4.386IleThr: 4.386 ± 0.099
4.528IleVal: 4.528 ± 0.063
0.782IleTrp: 0.782 ± 0.027
2.707IleTyr: 2.707 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
5.127LysAla: 5.127 ± 0.07
0.396LysCys: 0.396 ± 0.019
4.214LysAsp: 4.214 ± 0.062
5.334LysGlu: 5.334 ± 0.074
3.394LysPhe: 3.394 ± 0.056
4.628LysGly: 4.628 ± 0.074
1.187LysHis: 1.187 ± 0.03
7.017LysIle: 7.017 ± 0.087
6.917LysLys: 6.917 ± 0.089
6.737LysLeu: 6.737 ± 0.094
2.299LysMet: 2.299 ± 0.045
5.789LysAsn: 5.789 ± 0.084
2.93LysPro: 2.93 ± 0.05
2.255LysGln: 2.255 ± 0.05
2.513LysArg: 2.513 ± 0.055
5.195LysSer: 5.195 ± 0.069
4.901LysThr: 4.901 ± 0.066
5.007LysVal: 5.007 ± 0.077
0.877LysTrp: 0.877 ± 0.027
3.404LysTyr: 3.404 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
6.324LeuAla: 6.324 ± 0.091
0.67LeuCys: 0.67 ± 0.025
4.934LeuAsp: 4.934 ± 0.067
6.043LeuGlu: 6.043 ± 0.082
4.943LeuPhe: 4.943 ± 0.094
6.371LeuGly: 6.371 ± 0.083
1.387LeuHis: 1.387 ± 0.036
7.037LeuIle: 7.037 ± 0.09
8.147LeuLys: 8.147 ± 0.099
8.506LeuLeu: 8.506 ± 0.117
2.144LeuMet: 2.144 ± 0.049
5.708LeuAsn: 5.708 ± 0.07
3.76LeuPro: 3.76 ± 0.059
2.856LeuGln: 2.856 ± 0.05
3.128LeuArg: 3.128 ± 0.052
6.964LeuSer: 6.964 ± 0.073
4.924LeuThr: 4.924 ± 0.074
5.641LeuVal: 5.641 ± 0.082
0.856LeuTrp: 0.856 ± 0.029
2.769LeuTyr: 2.769 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
1.786MetAla: 1.786 ± 0.04
0.155MetCys: 0.155 ± 0.012
1.2MetAsp: 1.2 ± 0.033
1.397MetGlu: 1.397 ± 0.037
1.01MetPhe: 1.01 ± 0.032
1.677MetGly: 1.677 ± 0.042
0.429MetHis: 0.429 ± 0.019
1.502MetIle: 1.502 ± 0.042
2.235MetLys: 2.235 ± 0.045
2.023MetLeu: 2.023 ± 0.046
0.636MetMet: 0.636 ± 0.023
1.259MetAsn: 1.259 ± 0.032
1.041MetPro: 1.041 ± 0.03
0.782MetGln: 0.782 ± 0.025
0.844MetArg: 0.844 ± 0.029
1.465MetSer: 1.465 ± 0.034
1.165MetThr: 1.165 ± 0.032
1.5MetVal: 1.5 ± 0.04
0.216MetTrp: 0.216 ± 0.013
0.643MetTyr: 0.643 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.833AsnAla: 3.833 ± 0.065
0.516AsnCys: 0.516 ± 0.027
2.786AsnAsp: 2.786 ± 0.059
3.408AsnGlu: 3.408 ± 0.057
3.375AsnPhe: 3.375 ± 0.053
4.267AsnGly: 4.267 ± 0.088
1.05AsnHis: 1.05 ± 0.028
5.032AsnIle: 5.032 ± 0.063
4.229AsnLys: 4.229 ± 0.064
5.886AsnLeu: 5.886 ± 0.078
1.266AsnMet: 1.266 ± 0.033
3.673AsnAsn: 3.673 ± 0.073
3.002AsnPro: 3.002 ± 0.056
2.505AsnGln: 2.505 ± 0.057
2.081AsnArg: 2.081 ± 0.046
4.311AsnSer: 4.311 ± 0.079
3.464AsnThr: 3.464 ± 0.074
3.295AsnVal: 3.295 ± 0.061
0.791AsnTrp: 0.791 ± 0.026
2.734AsnTyr: 2.734 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
2.319ProAla: 2.319 ± 0.052
0.217ProCys: 0.217 ± 0.016
2.05ProAsp: 2.05 ± 0.05
2.767ProGlu: 2.767 ± 0.052
2.025ProPhe: 2.025 ± 0.045
2.105ProGly: 2.105 ± 0.048
0.628ProHis: 0.628 ± 0.021
2.872ProIle: 2.872 ± 0.052
2.835ProLys: 2.835 ± 0.05
3.042ProLeu: 3.042 ± 0.053
0.83ProMet: 0.83 ± 0.024
2.567ProAsn: 2.567 ± 0.055
0.964ProPro: 0.964 ± 0.034
1.173ProGln: 1.173 ± 0.032
0.957ProArg: 0.957 ± 0.028
2.5ProSer: 2.5 ± 0.049
2.185ProThr: 2.185 ± 0.063
2.382ProVal: 2.382 ± 0.054
0.4ProTrp: 0.4 ± 0.021
1.399ProTyr: 1.399 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
2.027GlnAla: 2.027 ± 0.045
0.175GlnCys: 0.175 ± 0.011
1.366GlnAsp: 1.366 ± 0.031
1.887GlnGlu: 1.887 ± 0.04
1.641GlnPhe: 1.641 ± 0.038
1.736GlnGly: 1.736 ± 0.042
0.496GlnHis: 0.496 ± 0.018
2.957GlnIle: 2.957 ± 0.049
3.314GlnLys: 3.314 ± 0.061
2.835GlnLeu: 2.835 ± 0.057
0.861GlnMet: 0.861 ± 0.028
2.53GlnAsn: 2.53 ± 0.05
1.098GlnPro: 1.098 ± 0.028
1.167GlnGln: 1.167 ± 0.033
1.128GlnArg: 1.128 ± 0.033
2.069GlnSer: 2.069 ± 0.045
2.046GlnThr: 2.046 ± 0.041
1.883GlnVal: 1.883 ± 0.038
0.365GlnTrp: 0.365 ± 0.02
1.239GlnTyr: 1.239 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
1.952ArgAla: 1.952 ± 0.047
0.231ArgCys: 0.231 ± 0.021
1.629ArgAsp: 1.629 ± 0.047
1.954ArgGlu: 1.954 ± 0.047
1.913ArgPhe: 1.913 ± 0.049
1.974ArgGly: 1.974 ± 0.044
0.527ArgHis: 0.527 ± 0.021
2.896ArgIle: 2.896 ± 0.047
2.808ArgLys: 2.808 ± 0.047
3.105ArgLeu: 3.105 ± 0.056
0.99ArgMet: 0.99 ± 0.029
2.085ArgAsn: 2.085 ± 0.049
1.168ArgPro: 1.168 ± 0.032
1.07ArgGln: 1.07 ± 0.033
1.307ArgArg: 1.307 ± 0.037
1.797ArgSer: 1.797 ± 0.041
1.8ArgThr: 1.8 ± 0.044
1.988ArgVal: 1.988 ± 0.044
0.408ArgTrp: 0.408 ± 0.017
1.296ArgTyr: 1.296 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
4.184SerAla: 4.184 ± 0.073
0.712SerCys: 0.712 ± 0.034
3.404SerAsp: 3.404 ± 0.054
4.117SerGlu: 4.117 ± 0.059
4.057SerPhe: 4.057 ± 0.065
5.076SerGly: 5.076 ± 0.09
1.136SerHis: 1.136 ± 0.032
5.214SerIle: 5.214 ± 0.071
5.37SerLys: 5.37 ± 0.075
6.399SerLeu: 6.399 ± 0.076
1.377SerMet: 1.377 ± 0.034
4.115SerAsn: 4.115 ± 0.109
2.546SerPro: 2.546 ± 0.056
2.299SerGln: 2.299 ± 0.043
2.15SerArg: 2.15 ± 0.044
4.639SerSer: 4.639 ± 0.086
3.81SerThr: 3.81 ± 0.094
4.201SerVal: 4.201 ± 0.064
0.768SerTrp: 0.768 ± 0.028
2.536SerTyr: 2.536 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
3.877ThrAla: 3.877 ± 0.146
0.48ThrCys: 0.48 ± 0.039
2.876ThrAsp: 2.876 ± 0.055
3.288ThrGlu: 3.288 ± 0.054
3.084ThrPhe: 3.084 ± 0.065
4.061ThrGly: 4.061 ± 0.093
0.954ThrHis: 0.954 ± 0.029
4.327ThrIle: 4.327 ± 0.103
4.011ThrLys: 4.011 ± 0.073
5.242ThrLeu: 5.242 ± 0.08
1.009ThrMet: 1.009 ± 0.032
3.306ThrAsn: 3.306 ± 0.084
2.376ThrPro: 2.376 ± 0.071
1.789ThrGln: 1.789 ± 0.041
1.518ThrArg: 1.518 ± 0.034
3.81ThrSer: 3.81 ± 0.097
3.222ThrThr: 3.222 ± 0.098
3.516ThrVal: 3.516 ± 0.098
0.749ThrTrp: 0.749 ± 0.036
2.261ThrTyr: 2.261 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
4.13ValAla: 4.13 ± 0.078
0.651ValCys: 0.651 ± 0.03
3.16ValAsp: 3.16 ± 0.047
3.847ValGlu: 3.847 ± 0.061
3.854ValPhe: 3.854 ± 0.065
4.319ValGly: 4.319 ± 0.086
0.892ValHis: 0.892 ± 0.026
4.416ValIle: 4.416 ± 0.068
4.495ValLys: 4.495 ± 0.068
5.827ValLeu: 5.827 ± 0.078
1.352ValMet: 1.352 ± 0.035
3.586ValAsn: 3.586 ± 0.058
2.237ValPro: 2.237 ± 0.042
1.571ValGln: 1.571 ± 0.037
1.957ValArg: 1.957 ± 0.041
4.514ValSer: 4.514 ± 0.067
3.07ValThr: 3.07 ± 0.093
4.243ValVal: 4.243 ± 0.071
0.697ValTrp: 0.697 ± 0.025
2.243ValTyr: 2.243 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.698TrpAla: 0.698 ± 0.025
0.086TrpCys: 0.086 ± 0.009
0.626TrpAsp: 0.626 ± 0.023
0.724TrpGlu: 0.724 ± 0.025
0.581TrpPhe: 0.581 ± 0.023
0.825TrpGly: 0.825 ± 0.028
0.24TrpHis: 0.24 ± 0.014
0.805TrpIle: 0.805 ± 0.029
0.903TrpLys: 0.903 ± 0.029
1.051TrpLeu: 1.051 ± 0.03
0.349TrpMet: 0.349 ± 0.018
0.676TrpAsn: 0.676 ± 0.026
0.299TrpPro: 0.299 ± 0.017
0.46TrpGln: 0.46 ± 0.022
0.458TrpArg: 0.458 ± 0.023
0.834TrpSer: 0.834 ± 0.042
0.633TrpThr: 0.633 ± 0.026
0.754TrpVal: 0.754 ± 0.024
0.183TrpTrp: 0.183 ± 0.016
0.434TrpTyr: 0.434 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.336TyrAla: 2.336 ± 0.043
0.354TyrCys: 0.354 ± 0.015
2.113TyrAsp: 2.113 ± 0.045
2.12TyrGlu: 2.12 ± 0.047
2.678TyrPhe: 2.678 ± 0.048
2.553TyrGly: 2.553 ± 0.049
0.708TyrHis: 0.708 ± 0.028
2.359TyrIle: 2.359 ± 0.046
2.724TyrLys: 2.724 ± 0.048
3.935TyrLeu: 3.935 ± 0.064
0.73TyrMet: 0.73 ± 0.024
2.197TyrAsn: 2.197 ± 0.05
1.459TyrPro: 1.459 ± 0.037
1.543TyrGln: 1.543 ± 0.04
1.458TyrArg: 1.458 ± 0.035
2.665TyrSer: 2.665 ± 0.05
2.157TyrThr: 2.157 ± 0.065
2.027TyrVal: 2.027 ± 0.043
0.482TyrTrp: 0.482 ± 0.021
1.721TyrTyr: 1.721 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3514 proteins (1214332 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski