Reinekea forsetii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Saccharospirillaceae; Reinekea

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3259 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8KN44|A0A2K8KN44_9GAMM Proteorhodopsin OS=Reinekea forsetii OX=1336806 GN=REIFOR_00373 PE=3 SV=1
MM1 pKa = 7.54ASIGVLLGCAQPAGSDD17 pKa = 3.37TSGTTDD23 pKa = 2.95GTADD27 pKa = 3.19ATIYY31 pKa = 10.3RR32 pKa = 11.84QIAGTSALGRR42 pKa = 11.84AGSWSSNCLGSDD54 pKa = 4.02LASEE58 pKa = 4.75CARR61 pKa = 11.84ADD63 pKa = 3.55SEE65 pKa = 4.78GNYY68 pKa = 10.43LLSTPAEE75 pKa = 4.19QSQLMWSDD83 pKa = 3.12IPEE86 pKa = 4.31SDD88 pKa = 3.13GTTVRR93 pKa = 11.84LYY95 pKa = 10.94SRR97 pKa = 11.84YY98 pKa = 9.02RR99 pKa = 11.84HH100 pKa = 5.62QDD102 pKa = 2.78GLTSALININPSTHH116 pKa = 6.26AVLDD120 pKa = 3.33IWSNSQQGQSLEE132 pKa = 4.2LCAEE136 pKa = 4.03EE137 pKa = 4.44ATCIDD142 pKa = 3.67ALLAGFSAAVEE153 pKa = 4.11QTIVAQLDD161 pKa = 3.85EE162 pKa = 4.9LLGEE166 pKa = 4.66AWPDD170 pKa = 3.25GRR172 pKa = 11.84DD173 pKa = 3.65PFDD176 pKa = 4.43DD177 pKa = 4.75VYY179 pKa = 11.5LADD182 pKa = 5.39PDD184 pKa = 5.08LDD186 pKa = 4.23EE187 pKa = 6.45LDD189 pKa = 4.96LMHH192 pKa = 7.23DD193 pKa = 3.44TLGFVVSSSDD203 pKa = 4.33LSVIDD208 pKa = 3.61NAGEE212 pKa = 3.96ILTQVSLGRR221 pKa = 11.84LLQAVEE227 pKa = 4.3LADD230 pKa = 3.81LTLTSAQISAAQALPIPDD248 pKa = 4.21PEE250 pKa = 5.95IIVISLRR257 pKa = 11.84MSVSPTLATEE267 pKa = 4.15APVDD271 pKa = 3.65VLVSDD276 pKa = 4.74RR277 pKa = 11.84GSSSVAGGEE286 pKa = 4.19LTFSHH291 pKa = 7.7DD292 pKa = 3.23LTLANGASLSFSGADD307 pKa = 3.08VVTTITAGGDD317 pKa = 3.68HH318 pKa = 6.48NWVVTATDD326 pKa = 3.6INDD329 pKa = 3.46FSITQGYY336 pKa = 8.99VISVLNGSDD345 pKa = 3.44GVATFGGAGSCLTPLTGLNANALNQCHH372 pKa = 5.54EE373 pKa = 4.49VQNGTALGSCDD384 pKa = 3.61QLDD387 pKa = 3.88SGSVSLSDD395 pKa = 4.54SPAPCARR402 pKa = 11.84EE403 pKa = 3.98VQNEE407 pKa = 3.94GALFGVCTWVDD418 pKa = 3.06SEE420 pKa = 4.73LRR422 pKa = 11.84VFHH425 pKa = 6.18YY426 pKa = 8.87EE427 pKa = 3.63NPLRR431 pKa = 11.84LNLVEE436 pKa = 5.23LFSEE440 pKa = 4.33RR441 pKa = 11.84QSRR444 pKa = 11.84IATEE448 pKa = 4.61CISDD452 pKa = 3.88VGNLWSISPP461 pKa = 3.95

Molecular weight: 48.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8KVS9|A0A2K8KVS9_9GAMM Kinase OS=Reinekea forsetii OX=1336806 GN=REIFOR_02728 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.01RR12 pKa = 11.84ARR14 pKa = 11.84SHH16 pKa = 6.1GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.43NGRR28 pKa = 11.84KK29 pKa = 9.65LIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84ALLSAA44 pKa = 4.86

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3259

0

3259

1072189

37

3058

329.0

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.1 ± 0.064

0.944 ± 0.014

5.734 ± 0.041

5.395 ± 0.042

3.974 ± 0.032

7.211 ± 0.049

2.219 ± 0.021

5.929 ± 0.034

3.825 ± 0.034

11.23 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.02

3.658 ± 0.026

4.262 ± 0.028

4.799 ± 0.036

5.414 ± 0.037

6.354 ± 0.035

5.342 ± 0.037

6.99 ± 0.032

1.371 ± 0.019

2.784 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski