Sinorhizobium phage phiM6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346HWD8|A0A346HWD8_9CAUD Uncharacterized protein OS=Sinorhizobium phage phiM6 OX=2301527 GN=SmphiM6_114 PE=4 SV=1
MM1 pKa = 7.42YY2 pKa = 10.35ILYY5 pKa = 9.94QPNLVVPACFEE16 pKa = 4.26VGDD19 pKa = 3.85DD20 pKa = 4.68FYY22 pKa = 11.34WVMKK26 pKa = 10.34PMGPSACQWIVPGTVDD42 pKa = 3.92PRR44 pKa = 11.84EE45 pKa = 4.15DD46 pKa = 3.09

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346HW57|A0A346HW57_9CAUD Uncharacterized protein OS=Sinorhizobium phage phiM6 OX=2301527 GN=SmphiM6_33 PE=4 SV=1
MM1 pKa = 7.67IEE3 pKa = 4.65LIKK6 pKa = 10.77HH7 pKa = 5.7SSTSYY12 pKa = 10.89SGILNSTTDD21 pKa = 3.16RR22 pKa = 11.84ATSHH26 pKa = 5.88LTKK29 pKa = 10.8AHH31 pKa = 6.54IALTFHH37 pKa = 6.68PSFRR41 pKa = 11.84EE42 pKa = 4.49GYY44 pKa = 8.82YY45 pKa = 10.12SVLVGRR51 pKa = 11.84STSDD55 pKa = 3.36CVSSKK60 pKa = 9.81VRR62 pKa = 11.84ITLIVAFILFDD73 pKa = 3.47IPLEE77 pKa = 3.93VLFRR81 pKa = 11.84ITKK84 pKa = 9.09INILFVHH91 pKa = 5.82SRR93 pKa = 11.84GRR95 pKa = 11.84HH96 pKa = 4.73PIVFIGHH103 pKa = 6.85LMISRR108 pKa = 11.84PRR110 pKa = 11.84DD111 pKa = 3.11ASNRR115 pKa = 11.84ITNILRR121 pKa = 11.84SYY123 pKa = 9.13SCNRR127 pKa = 11.84RR128 pKa = 11.84FNFLSYY134 pKa = 10.66RR135 pKa = 11.84SLISLIPRR143 pKa = 11.84VKK145 pKa = 10.6LLVFTFSGSNITFIPRR161 pKa = 11.84VEE163 pKa = 4.12FFHH166 pKa = 6.85YY167 pKa = 7.82FTSSS171 pKa = 2.57

Molecular weight: 19.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

121

0

121

21263

38

1077

175.7

19.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.05 ± 0.357

1.011 ± 0.123

6.768 ± 0.205

7.28 ± 0.35

4.035 ± 0.169

7.2 ± 0.332

2.173 ± 0.161

5.954 ± 0.211

7.271 ± 0.337

7.407 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.105

4.731 ± 0.206

4.209 ± 0.201

3.56 ± 0.247

5.347 ± 0.217

5.973 ± 0.354

5.742 ± 0.39

6.448 ± 0.203

1.519 ± 0.13

3.711 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski