Mycoplasma mustelae
Average proteome isoelectric point is 7.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 686 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R7UC52|A0A4R7UC52_9MOLU DNA (cytosine-5-)-methyltransferase OS=Mycoplasma mustelae OX=171289 GN=BCF59_0510 PE=3 SV=1
MM1 pKa = 7.46 WPNEE5 pKa = 4.12 VIYY8 pKa = 10.74 DD9 pKa = 3.86 WLQQLKK15 pKa = 10.39 QNLAIADD22 pKa = 3.96 DD23 pKa = 4.06 SRR25 pKa = 11.84 DD26 pKa = 4.0 DD27 pKa = 4.29 LLLDD31 pKa = 3.86 YY32 pKa = 11.18 IEE34 pKa = 4.57 IARR37 pKa = 11.84 EE38 pKa = 4.14 NIWKK42 pKa = 9.9 QYY44 pKa = 10.88 YY45 pKa = 9.51 EE46 pKa = 4.94 LEE48 pKa = 4.11 TDD50 pKa = 3.41 EE51 pKa = 5.74 NGIPDD56 pKa = 3.91 EE57 pKa = 5.36 HH58 pKa = 6.69 PWSWDD63 pKa = 3.14 LKK65 pKa = 8.66 TKK67 pKa = 10.59 LATLHH72 pKa = 6.59 LAALYY77 pKa = 10.41 VSNPDD82 pKa = 3.31 TNNIANSVIDD92 pKa = 3.2 SRR94 pKa = 11.84 MIFKK98 pKa = 10.57 ILGDD102 pKa = 4.39 RR103 pKa = 11.84 IPYY106 pKa = 9.01 GKK108 pKa = 10.01 NN109 pKa = 2.74
Molecular weight: 12.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.415
IPC2_protein 4.393
IPC_protein 4.317
Toseland 4.126
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.215
Rodwell 4.151
Grimsley 4.037
Solomon 4.291
Lehninger 4.24
Nozaki 4.406
DTASelect 4.635
Thurlkill 4.164
EMBOSS 4.228
Sillero 4.431
Patrickios 3.719
IPC_peptide 4.291
IPC2_peptide 4.418
IPC2.peptide.svr19 4.369
Protein with the highest isoelectric point:
>tr|A0A4R7UDW7|A0A4R7UDW7_9MOLU Cell division protein FtsZ OS=Mycoplasma mustelae OX=171289 GN=ftsZ PE=3 SV=1
MM1 pKa = 7.39 ARR3 pKa = 11.84 KK4 pKa = 9.49 ALIIKK9 pKa = 9.08 SKK11 pKa = 9.75 RR12 pKa = 11.84 HH13 pKa = 5.46 SKK15 pKa = 10.45 FSTRR19 pKa = 11.84 AYY21 pKa = 8.59 TRR23 pKa = 11.84 CEE25 pKa = 3.8 LCGRR29 pKa = 11.84 PHH31 pKa = 7.07 AVLRR35 pKa = 11.84 KK36 pKa = 9.46 YY37 pKa = 10.22 KK38 pKa = 9.85 ICRR41 pKa = 11.84 ICFRR45 pKa = 11.84 NLAHH49 pKa = 6.91 EE50 pKa = 4.49 GKK52 pKa = 10.2 IPGIKK57 pKa = 9.11 KK58 pKa = 10.56 ASWW61 pKa = 2.94
Molecular weight: 7.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.911
IPC_protein 10.643
Toseland 11.067
ProMoST 10.745
Dawson 11.125
Bjellqvist 10.818
Wikipedia 11.316
Rodwell 11.462
Grimsley 11.14
Solomon 11.272
Lehninger 11.242
Nozaki 11.052
DTASelect 10.818
Thurlkill 11.038
EMBOSS 11.462
Sillero 11.052
Patrickios 11.199
IPC_peptide 11.286
IPC2_peptide 10.014
IPC2.peptide.svr19 8.448
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
686
0
686
251183
37
3344
366.2
41.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.335 ± 0.093
0.461 ± 0.023
5.433 ± 0.087
5.952 ± 0.087
5.624 ± 0.09
4.625 ± 0.094
1.487 ± 0.034
9.489 ± 0.114
9.882 ± 0.084
9.885 ± 0.098
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.655 ± 0.038
7.808 ± 0.128
2.636 ± 0.054
4.063 ± 0.072
2.965 ± 0.058
6.537 ± 0.062
5.434 ± 0.078
5.489 ± 0.074
0.983 ± 0.032
4.255 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here