Aeromonas virus 44RR2
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 252 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6U9H7|Q6U9H7_9CAUD Uncharacterized protein OS=Aeromonas virus 44RR2 OX=115987 GN=44RRORF125c PE=4 SV=1
MM1 pKa = 7.94 TDD3 pKa = 3.06 QQEE6 pKa = 4.24 LVMQFRR12 pKa = 11.84 PSSTIKK18 pKa = 9.71 IWCEE22 pKa = 3.41 YY23 pKa = 10.3 DD24 pKa = 2.88 ISGQFGGNNNEE35 pKa = 4.05 EE36 pKa = 4.43 VVSITFPEE44 pKa = 4.32 HH45 pKa = 4.6 VQEE48 pKa = 4.18 NEE50 pKa = 3.7 RR51 pKa = 11.84 NGYY54 pKa = 9.31 IEE56 pKa = 5.63 DD57 pKa = 3.7 VVLTYY62 pKa = 8.99 VQKK65 pKa = 8.29 MTYY68 pKa = 10.08 EE69 pKa = 4.46 DD70 pKa = 4.11 ADD72 pKa = 4.07 SLEE75 pKa = 4.2 GLYY78 pKa = 10.49 GWEE81 pKa = 5.04 HH82 pKa = 7.41 IDD84 pKa = 4.45 IVNLL88 pKa = 3.6
Molecular weight: 10.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 4.062
IPC_protein 3.948
Toseland 3.783
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.795
Rodwell 3.783
Grimsley 3.694
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.139
Thurlkill 3.808
EMBOSS 3.808
Sillero 4.062
Patrickios 0.846
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.951
Protein with the highest isoelectric point:
>tr|Q6U9E5|Q6U9E5_9CAUD Prohead core protein OS=Aeromonas virus 44RR2 OX=115987 GN=67 PE=4 SV=1
MM1 pKa = 7.96 LIPSDD6 pKa = 4.48 YY7 pKa = 10.26 IAEE10 pKa = 4.2 AVKK13 pKa = 10.4 IEE15 pKa = 4.25 AVMADD20 pKa = 2.73 ATFRR24 pKa = 11.84 VNHH27 pKa = 5.66 LVEE30 pKa = 4.89 SGFATDD36 pKa = 4.72 EE37 pKa = 4.02 IVGVISRR44 pKa = 11.84 VAASEE49 pKa = 3.84 PDD51 pKa = 3.2 MAIAMISVVEE61 pKa = 4.22 STSIDD66 pKa = 3.55 EE67 pKa = 5.51 AIVKK71 pKa = 9.52 HH72 pKa = 5.4 VNSRR76 pKa = 11.84 GEE78 pKa = 3.65 ITRR81 pKa = 11.84 TKK83 pKa = 10.93 DD84 pKa = 2.54 RR85 pKa = 11.84 ATRR88 pKa = 11.84 KK89 pKa = 9.63 RR90 pKa = 11.84 NAFATTGLTASTRR103 pKa = 11.84 RR104 pKa = 11.84 QIARR108 pKa = 11.84 RR109 pKa = 11.84 AAKK112 pKa = 9.18 TKK114 pKa = 10.08 RR115 pKa = 11.84 ANPSISVKK123 pKa = 10.17 AQRR126 pKa = 11.84 KK127 pKa = 4.72 TKK129 pKa = 10.23 RR130 pKa = 11.84 ALKK133 pKa = 9.79 RR134 pKa = 11.84 RR135 pKa = 11.84 KK136 pKa = 9.77 ALGLL140 pKa = 3.7
Molecular weight: 15.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.721
IPC_protein 10.628
Toseland 11.14
ProMoST 11.082
Dawson 11.169
Bjellqvist 10.935
Wikipedia 11.433
Rodwell 11.345
Grimsley 11.184
Solomon 11.418
Lehninger 11.374
Nozaki 11.111
DTASelect 10.935
Thurlkill 11.111
EMBOSS 11.564
Sillero 11.111
Patrickios 11.082
IPC_peptide 11.433
IPC2_peptide 9.882
IPC2.peptide.svr19 8.916
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
252
0
252
53462
32
1420
212.2
23.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.241 ± 0.205
1.143 ± 0.065
6.41 ± 0.126
6.818 ± 0.217
4.523 ± 0.119
6.625 ± 0.204
2.076 ± 0.083
7.057 ± 0.142
6.586 ± 0.198
7.162 ± 0.124
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.114 ± 0.102
5.318 ± 0.123
3.646 ± 0.086
3.421 ± 0.093
4.807 ± 0.101
6.272 ± 0.148
5.709 ± 0.198
6.779 ± 0.126
1.429 ± 0.07
3.864 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here