Eggerthia catenaformis OT 569 = DSM 20559

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Coprobacillaceae; Eggerthia; Eggerthia catenaformis

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M2Q0F9|M2Q0F9_9FIRM Uncharacterized protein OS=Eggerthia catenaformis OT 569 = DSM 20559 OX=999415 GN=HMPREF9943_01341 PE=4 SV=1
MM1 pKa = 7.87PKK3 pKa = 10.08QIQTMTFDD11 pKa = 3.08NKK13 pKa = 9.29TYY15 pKa = 10.14EE16 pKa = 3.98IIGIFEE22 pKa = 4.28YY23 pKa = 10.74EE24 pKa = 4.07EE25 pKa = 4.07KK26 pKa = 10.7EE27 pKa = 4.29YY28 pKa = 10.64IALLTNDD35 pKa = 3.83KK36 pKa = 11.22KK37 pKa = 11.08EE38 pKa = 3.87DD39 pKa = 3.54VYY41 pKa = 11.48LFEE44 pKa = 5.38YY45 pKa = 9.75YY46 pKa = 9.89QEE48 pKa = 4.18NNHH51 pKa = 5.89YY52 pKa = 9.92EE53 pKa = 4.16LFDD56 pKa = 4.37IPDD59 pKa = 4.89DD60 pKa = 3.86IFDD63 pKa = 4.24DD64 pKa = 3.82VAEE67 pKa = 4.05EE68 pKa = 4.3FEE70 pKa = 5.27FIVEE74 pKa = 4.15NQLFDD79 pKa = 3.4

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M2PBC9|M2PBC9_9FIRM Uncharacterized protein OS=Eggerthia catenaformis OT 569 = DSM 20559 OX=999415 GN=HMPREF9943_00129 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.38GRR39 pKa = 11.84KK40 pKa = 8.82ALTVV44 pKa = 3.3

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1874

0

1874

565588

25

1743

301.8

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.802 ± 0.069

1.149 ± 0.021

5.907 ± 0.04

6.723 ± 0.061

4.381 ± 0.046

6.034 ± 0.05

2.302 ± 0.03

9.218 ± 0.054

8.618 ± 0.066

9.351 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.03

5.386 ± 0.043

2.809 ± 0.031

3.073 ± 0.032

3.652 ± 0.04

6.244 ± 0.042

5.087 ± 0.036

5.838 ± 0.05

0.703 ± 0.017

4.894 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski