Streptomyces sp. AS58

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8128 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8VSS5|A0A0M8VSS5_9ACTN Hydrolase OS=Streptomyces sp. AS58 OX=1519489 GN=ADL00_11670 PE=4 SV=1
SS1 pKa = 6.94HH2 pKa = 7.36EE3 pKa = 4.53GYY5 pKa = 10.85NYY7 pKa = 10.34WFDD10 pKa = 4.09NDD12 pKa = 4.49DD13 pKa = 4.39LSSSLYY19 pKa = 10.31SGPRR23 pKa = 11.84EE24 pKa = 4.16PGSGVLDD31 pKa = 3.41QVPATLTVISRR42 pKa = 11.84SQDD45 pKa = 3.25VASSEE50 pKa = 3.98LAEE53 pKa = 4.6RR54 pKa = 11.84IYY56 pKa = 11.33AGLTASDD63 pKa = 4.66DD64 pKa = 3.79YY65 pKa = 11.59LVVVFAHH72 pKa = 6.3NGMPLAANFDD82 pKa = 3.99IGDD85 pKa = 3.64DD86 pKa = 3.52WW87 pKa = 6.17

Molecular weight: 9.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8V7U7|A0A0M8V7U7_9ACTN Uncharacterized protein OS=Streptomyces sp. AS58 OX=1519489 GN=ADL00_25475 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84HH3 pKa = 6.13PATLRR8 pKa = 11.84RR9 pKa = 11.84ARR11 pKa = 11.84LRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ASHH18 pKa = 6.89AAPNSAPTGPRR29 pKa = 11.84AEE31 pKa = 4.19RR32 pKa = 11.84GRR34 pKa = 11.84PLAARR39 pKa = 11.84SRR41 pKa = 11.84RR42 pKa = 11.84GPAGVSAAPPATPAPRR58 pKa = 11.84GSPARR63 pKa = 11.84RR64 pKa = 11.84TPSPAA69 pKa = 3.0

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8128

0

8128

2609829

21

9828

321.1

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.417 ± 0.041

0.791 ± 0.008

6.145 ± 0.023

5.784 ± 0.026

2.724 ± 0.014

9.316 ± 0.027

2.362 ± 0.015

3.135 ± 0.021

2.021 ± 0.021

10.327 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.71 ± 0.011

1.766 ± 0.015

6.048 ± 0.028

2.785 ± 0.016

8.269 ± 0.034

5.072 ± 0.02

6.15 ± 0.024

8.585 ± 0.027

1.532 ± 0.01

2.062 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski