Amino acid dipepetide frequency for Streptomyces sp. AS58

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.917AlaAla: 20.917 ± 0.153
1.075AlaCys: 1.075 ± 0.02
8.449AlaAsp: 8.449 ± 0.076
8.891AlaGlu: 8.891 ± 0.078
3.458AlaPhe: 3.458 ± 0.038
12.829AlaGly: 12.829 ± 0.091
2.948AlaHis: 2.948 ± 0.034
3.289AlaIle: 3.289 ± 0.042
2.538AlaLys: 2.538 ± 0.046
14.32AlaLeu: 14.32 ± 0.11
2.393AlaMet: 2.393 ± 0.034
1.9AlaAsn: 1.9 ± 0.03
6.951AlaPro: 6.951 ± 0.063
3.863AlaGln: 3.863 ± 0.043
10.662AlaArg: 10.662 ± 0.085
5.838AlaSer: 5.838 ± 0.048
6.843AlaThr: 6.843 ± 0.053
12.445AlaVal: 12.445 ± 0.085
1.892AlaTrp: 1.892 ± 0.027
2.665AlaTyr: 2.665 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
1.09CysAla: 1.09 ± 0.023
0.097CysCys: 0.097 ± 0.007
0.465CysAsp: 0.465 ± 0.013
0.408CysGlu: 0.408 ± 0.013
0.217CysPhe: 0.217 ± 0.01
0.947CysGly: 0.947 ± 0.023
0.197CysHis: 0.197 ± 0.008
0.173CysIle: 0.173 ± 0.009
0.104CysLys: 0.104 ± 0.006
0.768CysLeu: 0.768 ± 0.019
0.12CysMet: 0.12 ± 0.006
0.134CysAsn: 0.134 ± 0.008
0.493CysPro: 0.493 ± 0.015
0.168CysGln: 0.168 ± 0.008
0.642CysArg: 0.642 ± 0.018
0.434CysSer: 0.434 ± 0.014
0.51CysThr: 0.51 ± 0.015
0.666CysVal: 0.666 ± 0.016
0.133CysTrp: 0.133 ± 0.007
0.145CysTyr: 0.145 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.635AspAla: 7.635 ± 0.056
0.459AspCys: 0.459 ± 0.015
3.834AspAsp: 3.834 ± 0.043
4.012AspGlu: 4.012 ± 0.037
1.692AspPhe: 1.692 ± 0.027
6.553AspGly: 6.553 ± 0.057
1.468AspHis: 1.468 ± 0.024
1.931AspIle: 1.931 ± 0.032
1.148AspLys: 1.148 ± 0.024
6.453AspLeu: 6.453 ± 0.055
0.84AspMet: 0.84 ± 0.02
0.994AspAsn: 0.994 ± 0.023
4.52AspPro: 4.52 ± 0.046
1.535AspGln: 1.535 ± 0.026
5.221AspArg: 5.221 ± 0.05
2.627AspSer: 2.627 ± 0.031
3.346AspThr: 3.346 ± 0.037
5.016AspVal: 5.016 ± 0.049
1.056AspTrp: 1.056 ± 0.018
1.105AspTyr: 1.105 ± 0.02
0.0AspXaa: 0.0 ± 0.0
Glu
7.423GluAla: 7.423 ± 0.072
0.387GluCys: 0.387 ± 0.013
2.962GluAsp: 2.962 ± 0.037
3.571GluGlu: 3.571 ± 0.044
1.553GluPhe: 1.553 ± 0.026
4.279GluGly: 4.279 ± 0.042
1.577GluHis: 1.577 ± 0.027
2.315GluIle: 2.315 ± 0.034
1.401GluLys: 1.401 ± 0.026
6.897GluLeu: 6.897 ± 0.064
0.883GluMet: 0.883 ± 0.021
1.043GluAsn: 1.043 ± 0.021
3.496GluPro: 3.496 ± 0.043
2.325GluGln: 2.325 ± 0.031
5.584GluArg: 5.584 ± 0.061
2.636GluSer: 2.636 ± 0.038
2.943GluThr: 2.943 ± 0.036
4.593GluVal: 4.593 ± 0.041
0.82GluTrp: 0.82 ± 0.022
1.155GluTyr: 1.155 ± 0.02
0.0GluXaa: 0.0 ± 0.0
Phe
3.571PheAla: 3.571 ± 0.047
0.264PheCys: 0.264 ± 0.01
1.966PheAsp: 1.966 ± 0.033
1.474PheGlu: 1.474 ± 0.025
0.887PhePhe: 0.887 ± 0.018
2.99PheGly: 2.99 ± 0.038
0.621PheHis: 0.621 ± 0.014
0.713PheIle: 0.713 ± 0.019
0.487PheLys: 0.487 ± 0.015
2.591PheLeu: 2.591 ± 0.035
0.4PheMet: 0.4 ± 0.012
0.569PheAsn: 0.569 ± 0.015
1.38PhePro: 1.38 ± 0.023
0.696PheGln: 0.696 ± 0.016
1.877PheArg: 1.877 ± 0.03
1.429PheSer: 1.429 ± 0.022
2.038PheThr: 2.038 ± 0.03
2.301PheVal: 2.301 ± 0.03
0.422PheTrp: 0.422 ± 0.014
0.559PheTyr: 0.559 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
10.826GlyAla: 10.826 ± 0.079
0.819GlyCys: 0.819 ± 0.019
5.288GlyAsp: 5.288 ± 0.051
5.174GlyGlu: 5.174 ± 0.05
2.758GlyPhe: 2.758 ± 0.032
8.456GlyGly: 8.456 ± 0.075
2.398GlyHis: 2.398 ± 0.031
3.375GlyIle: 3.375 ± 0.04
2.265GlyLys: 2.265 ± 0.039
9.298GlyLeu: 9.298 ± 0.07
1.934GlyMet: 1.934 ± 0.025
1.722GlyAsn: 1.722 ± 0.032
5.136GlyPro: 5.136 ± 0.056
2.632GlyGln: 2.632 ± 0.034
7.978GlyArg: 7.978 ± 0.059
5.379GlySer: 5.379 ± 0.053
6.34GlyThr: 6.34 ± 0.059
7.485GlyVal: 7.485 ± 0.061
1.701GlyTrp: 1.701 ± 0.03
2.19GlyTyr: 2.19 ± 0.028
0.0GlyXaa: 0.0 ± 0.0
His
2.737HisAla: 2.737 ± 0.035
0.213HisCys: 0.213 ± 0.009
1.455HisAsp: 1.455 ± 0.025
1.284HisGlu: 1.284 ± 0.023
0.644HisPhe: 0.644 ± 0.015
2.487HisGly: 2.487 ± 0.032
0.719HisHis: 0.719 ± 0.018
0.725HisIle: 0.725 ± 0.017
0.344HisLys: 0.344 ± 0.012
2.496HisLeu: 2.496 ± 0.037
0.342HisMet: 0.342 ± 0.013
0.374HisAsn: 0.374 ± 0.011
1.811HisPro: 1.811 ± 0.027
0.668HisGln: 0.668 ± 0.017
2.244HisArg: 2.244 ± 0.033
1.072HisSer: 1.072 ± 0.02
1.386HisThr: 1.386 ± 0.023
1.757HisVal: 1.757 ± 0.026
0.385HisTrp: 0.385 ± 0.012
0.476HisTyr: 0.476 ± 0.013
0.0HisXaa: 0.0 ± 0.0
Ile
4.67IleAla: 4.67 ± 0.049
0.28IleCys: 0.28 ± 0.01
2.288IleAsp: 2.288 ± 0.032
2.005IleGlu: 2.005 ± 0.029
0.684IlePhe: 0.684 ± 0.019
3.496IleGly: 3.496 ± 0.043
0.615IleHis: 0.615 ± 0.016
0.843IleIle: 0.843 ± 0.02
0.707IleLys: 0.707 ± 0.019
2.382IleLeu: 2.382 ± 0.034
0.448IleMet: 0.448 ± 0.016
0.677IleAsn: 0.677 ± 0.018
1.744IlePro: 1.744 ± 0.023
0.707IleGln: 0.707 ± 0.017
2.323IleArg: 2.323 ± 0.029
1.625IleSer: 1.625 ± 0.028
2.237IleThr: 2.237 ± 0.033
2.731IleVal: 2.731 ± 0.036
0.388IleTrp: 0.388 ± 0.012
0.505IleTyr: 0.505 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.709LysAla: 2.709 ± 0.042
0.114LysCys: 0.114 ± 0.008
1.227LysAsp: 1.227 ± 0.027
1.146LysGlu: 1.146 ± 0.026
0.436LysPhe: 0.436 ± 0.016
1.742LysGly: 1.742 ± 0.032
0.411LysHis: 0.411 ± 0.014
0.794LysIle: 0.794 ± 0.017
0.769LysLys: 0.769 ± 0.028
1.891LysLeu: 1.891 ± 0.032
0.353LysMet: 0.353 ± 0.014
0.499LysAsn: 0.499 ± 0.015
1.241LysPro: 1.241 ± 0.027
0.673LysGln: 0.673 ± 0.018
1.377LysArg: 1.377 ± 0.028
1.089LysSer: 1.089 ± 0.026
1.182LysThr: 1.182 ± 0.028
1.832LysVal: 1.832 ± 0.029
0.266LysTrp: 0.266 ± 0.013
0.459LysTyr: 0.459 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
14.952LeuAla: 14.952 ± 0.116
0.827LeuCys: 0.827 ± 0.021
6.924LeuAsp: 6.924 ± 0.055
4.748LeuGlu: 4.748 ± 0.05
2.562LeuPhe: 2.562 ± 0.036
9.03LeuGly: 9.03 ± 0.067
2.345LeuHis: 2.345 ± 0.032
3.327LeuIle: 3.327 ± 0.039
1.975LeuLys: 1.975 ± 0.032
11.283LeuLeu: 11.283 ± 0.087
1.652LeuMet: 1.652 ± 0.029
1.738LeuAsn: 1.738 ± 0.028
6.592LeuPro: 6.592 ± 0.057
2.283LeuGln: 2.283 ± 0.03
8.871LeuArg: 8.871 ± 0.076
5.369LeuSer: 5.369 ± 0.042
6.89LeuThr: 6.89 ± 0.053
8.767LeuVal: 8.767 ± 0.072
1.266LeuTrp: 1.266 ± 0.022
1.874LeuTyr: 1.874 ± 0.027
0.0LeuXaa: 0.0 ± 0.0
Met
2.231MetAla: 2.231 ± 0.034
0.133MetCys: 0.133 ± 0.006
0.883MetAsp: 0.883 ± 0.018
0.751MetGlu: 0.751 ± 0.018
0.454MetPhe: 0.454 ± 0.013
1.254MetGly: 1.254 ± 0.022
0.372MetHis: 0.372 ± 0.012
0.639MetIle: 0.639 ± 0.017
0.395MetLys: 0.395 ± 0.013
1.698MetLeu: 1.698 ± 0.024
0.295MetMet: 0.295 ± 0.012
0.438MetAsn: 0.438 ± 0.013
1.098MetPro: 1.098 ± 0.021
0.449MetGln: 0.449 ± 0.013
1.454MetArg: 1.454 ± 0.023
1.237MetSer: 1.237 ± 0.021
1.474MetThr: 1.474 ± 0.026
1.301MetVal: 1.301 ± 0.022
0.211MetTrp: 0.211 ± 0.009
0.331MetTyr: 0.331 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.168AsnAla: 2.168 ± 0.034
0.169AsnCys: 0.169 ± 0.008
0.967AsnAsp: 0.967 ± 0.019
0.815AsnGlu: 0.815 ± 0.022
0.511AsnPhe: 0.511 ± 0.014
1.882AsnGly: 1.882 ± 0.037
0.403AsnHis: 0.403 ± 0.015
0.629AsnIle: 0.629 ± 0.017
0.4AsnLys: 0.4 ± 0.014
1.628AsnLeu: 1.628 ± 0.028
0.296AsnMet: 0.296 ± 0.011
0.449AsnAsn: 0.449 ± 0.015
1.355AsnPro: 1.355 ± 0.022
0.499AsnGln: 0.499 ± 0.015
1.33AsnArg: 1.33 ± 0.023
0.952AsnSer: 0.952 ± 0.019
1.125AsnThr: 1.125 ± 0.026
1.378AsnVal: 1.378 ± 0.026
0.289AsnTrp: 0.289 ± 0.01
0.41AsnTyr: 0.41 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.503ProAla: 8.503 ± 0.074
0.336ProCys: 0.336 ± 0.012
4.607ProAsp: 4.607 ± 0.048
4.368ProGlu: 4.368 ± 0.046
1.563ProPhe: 1.563 ± 0.024
6.652ProGly: 6.652 ± 0.061
1.424ProHis: 1.424 ± 0.024
1.281ProIle: 1.281 ± 0.027
1.076ProLys: 1.076 ± 0.026
5.338ProLeu: 5.338 ± 0.051
0.975ProMet: 0.975 ± 0.022
0.871ProAsn: 0.871 ± 0.015
3.478ProPro: 3.478 ± 0.055
1.731ProGln: 1.731 ± 0.028
4.058ProArg: 4.058 ± 0.044
3.168ProSer: 3.168 ± 0.045
3.188ProThr: 3.188 ± 0.036
5.569ProVal: 5.569 ± 0.047
0.888ProTrp: 0.888 ± 0.019
1.406ProTyr: 1.406 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.686GlnAla: 3.686 ± 0.043
0.171GlnCys: 0.171 ± 0.008
1.487GlnAsp: 1.487 ± 0.027
1.506GlnGlu: 1.506 ± 0.024
0.703GlnPhe: 0.703 ± 0.018
2.256GlnGly: 2.256 ± 0.035
0.689GlnHis: 0.689 ± 0.018
1.11GlnIle: 1.11 ± 0.021
0.579GlnLys: 0.579 ± 0.014
3.017GlnLeu: 3.017 ± 0.031
0.489GlnMet: 0.489 ± 0.014
0.508GlnAsn: 0.508 ± 0.015
1.677GlnPro: 1.677 ± 0.028
1.259GlnGln: 1.259 ± 0.03
2.508GlnArg: 2.508 ± 0.037
1.334GlnSer: 1.334 ± 0.024
1.351GlnThr: 1.351 ± 0.026
2.425GlnVal: 2.425 ± 0.028
0.467GlnTrp: 0.467 ± 0.013
0.628GlnTyr: 0.628 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
10.228ArgAla: 10.228 ± 0.083
0.643ArgCys: 0.643 ± 0.017
4.477ArgAsp: 4.477 ± 0.039
4.784ArgGlu: 4.784 ± 0.048
2.41ArgPhe: 2.41 ± 0.035
5.908ArgGly: 5.908 ± 0.053
2.283ArgHis: 2.283 ± 0.034
3.311ArgIle: 3.311 ± 0.041
1.587ArgLys: 1.587 ± 0.029
9.224ArgLeu: 9.224 ± 0.074
1.741ArgMet: 1.741 ± 0.028
1.399ArgAsn: 1.399 ± 0.024
5.177ArgPro: 5.177 ± 0.065
2.394ArgGln: 2.394 ± 0.029
7.95ArgArg: 7.95 ± 0.079
4.255ArgSer: 4.255 ± 0.042
5.622ArgThr: 5.622 ± 0.054
6.064ArgVal: 6.064 ± 0.054
1.42ArgTrp: 1.42 ± 0.022
1.811ArgTyr: 1.811 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.844SerAla: 6.844 ± 0.053
0.402SerCys: 0.402 ± 0.013
2.829SerAsp: 2.829 ± 0.035
2.547SerGlu: 2.547 ± 0.031
1.518SerPhe: 1.518 ± 0.02
5.906SerGly: 5.906 ± 0.054
1.033SerHis: 1.033 ± 0.018
1.396SerIle: 1.396 ± 0.026
0.946SerLys: 0.946 ± 0.023
4.804SerLeu: 4.804 ± 0.046
1.058SerMet: 1.058 ± 0.022
0.861SerAsn: 0.861 ± 0.02
3.108SerPro: 3.108 ± 0.037
1.27SerGln: 1.27 ± 0.021
3.77SerArg: 3.77 ± 0.037
2.779SerSer: 2.779 ± 0.042
3.091SerThr: 3.091 ± 0.037
4.445SerVal: 4.445 ± 0.048
0.898SerTrp: 0.898 ± 0.021
1.218SerTyr: 1.218 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.027ThrAla: 9.027 ± 0.06
0.438ThrCys: 0.438 ± 0.014
3.756ThrAsp: 3.756 ± 0.043
3.391ThrGlu: 3.391 ± 0.036
1.613ThrPhe: 1.613 ± 0.021
6.699ThrGly: 6.699 ± 0.052
1.263ThrHis: 1.263 ± 0.021
1.644ThrIle: 1.644 ± 0.03
1.094ThrLys: 1.094 ± 0.029
5.762ThrLeu: 5.762 ± 0.05
0.949ThrMet: 0.949 ± 0.018
0.987ThrAsn: 0.987 ± 0.024
4.034ThrPro: 4.034 ± 0.046
1.34ThrGln: 1.34 ± 0.024
4.024ThrArg: 4.024 ± 0.044
3.164ThrSer: 3.164 ± 0.042
3.778ThrThr: 3.778 ± 0.048
6.29ThrVal: 6.29 ± 0.058
0.923ThrTrp: 0.923 ± 0.02
1.327ThrTyr: 1.327 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
10.606ValAla: 10.606 ± 0.079
0.741ValCys: 0.741 ± 0.018
5.199ValAsp: 5.199 ± 0.047
4.936ValGlu: 4.936 ± 0.049
2.431ValPhe: 2.431 ± 0.032
6.543ValGly: 6.543 ± 0.061
2.073ValHis: 2.073 ± 0.03
2.776ValIle: 2.776 ± 0.035
1.689ValLys: 1.689 ± 0.033
9.5ValLeu: 9.5 ± 0.077
1.404ValMet: 1.404 ± 0.023
1.689ValAsn: 1.689 ± 0.028
5.37ValPro: 5.37 ± 0.044
2.12ValGln: 2.12 ± 0.032
7.586ValArg: 7.586 ± 0.054
4.408ValSer: 4.408 ± 0.04
5.871ValThr: 5.871 ± 0.063
8.182ValVal: 8.182 ± 0.078
1.186ValTrp: 1.186 ± 0.022
1.542ValTyr: 1.542 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.667TrpAla: 1.667 ± 0.024
0.171TrpCys: 0.171 ± 0.008
0.833TrpAsp: 0.833 ± 0.02
0.749TrpGlu: 0.749 ± 0.017
0.506TrpPhe: 0.506 ± 0.016
1.044TrpGly: 1.044 ± 0.021
0.386TrpHis: 0.386 ± 0.011
0.586TrpIle: 0.586 ± 0.016
0.336TrpLys: 0.336 ± 0.011
1.806TrpLeu: 1.806 ± 0.034
0.274TrpMet: 0.274 ± 0.01
0.414TrpAsn: 0.414 ± 0.013
0.784TrpPro: 0.784 ± 0.019
0.646TrpGln: 0.646 ± 0.015
1.364TrpArg: 1.364 ± 0.026
0.971TrpSer: 0.971 ± 0.022
1.079TrpThr: 1.079 ± 0.023
0.969TrpVal: 0.969 ± 0.018
0.349TrpTrp: 0.349 ± 0.012
0.381TrpTyr: 0.381 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.678TyrAla: 2.678 ± 0.031
0.169TyrCys: 0.169 ± 0.008
1.55TyrAsp: 1.55 ± 0.032
1.283TyrGlu: 1.283 ± 0.023
0.634TyrPhe: 0.634 ± 0.015
2.204TyrGly: 2.204 ± 0.034
0.392TyrHis: 0.392 ± 0.013
0.497TyrIle: 0.497 ± 0.014
0.37TyrLys: 0.37 ± 0.012
2.117TyrLeu: 2.117 ± 0.029
0.251TyrMet: 0.251 ± 0.009
0.391TyrAsn: 0.391 ± 0.013
1.038TyrPro: 1.038 ± 0.019
0.593TyrGln: 0.593 ± 0.016
1.865TyrArg: 1.865 ± 0.03
0.957TyrSer: 0.957 ± 0.019
1.207TyrThr: 1.207 ± 0.027
1.636TyrVal: 1.636 ± 0.027
0.356TyrTrp: 0.356 ± 0.012
0.433TyrTyr: 0.433 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8128 proteins (2609829 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski