Pseudozyma antarctica (Yeast) (Candida antarctica)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Moesziomyces

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081CKH7|A0A081CKH7_PSEA2 Probable Shwachman-Bodian-Diamond syndrome protein OS=Pseudozyma antarctica OX=84753 GN=PAN0_017c5399 PE=3 SV=1
MM1 pKa = 7.37PVSSASNLVPGASVSLLGFASLAFVVHH28 pKa = 6.47CALFEE33 pKa = 4.4GEE35 pKa = 4.51FLRR38 pKa = 11.84LIAVSAMWSVLVATPLCICAAAAQCIDD65 pKa = 3.69CARR68 pKa = 11.84AAGCTDD74 pKa = 3.45CHH76 pKa = 6.25QAFDD80 pKa = 4.09ARR82 pKa = 11.84ASNSSADD89 pKa = 3.36FDD91 pKa = 4.18RR92 pKa = 11.84PEE94 pKa = 3.68RR95 pKa = 11.84TYY97 pKa = 10.83PVAVKK102 pKa = 9.02TLNLAGFFDD111 pKa = 5.14CLDD114 pKa = 3.91APDD117 pKa = 4.52YY118 pKa = 11.09PSDD121 pKa = 3.7QIIYY125 pKa = 10.09AVDD128 pKa = 3.41RR129 pKa = 11.84QGVFIEE135 pKa = 4.12VDD137 pKa = 2.98MGDD140 pKa = 3.93LLEE143 pKa = 4.35HH144 pKa = 6.79ARR146 pKa = 11.84RR147 pKa = 11.84PTQDD151 pKa = 3.37CNHH154 pKa = 5.53VVTDD158 pKa = 3.57EE159 pKa = 4.41TTVLDD164 pKa = 4.29YY165 pKa = 10.39LTLFHH170 pKa = 6.87GPSLGGRR177 pKa = 11.84SVALAAEE184 pKa = 4.76DD185 pKa = 3.67EE186 pKa = 4.87TIVGSNIDD194 pKa = 4.46PDD196 pKa = 3.91QSDD199 pKa = 4.31PDD201 pKa = 4.53PDD203 pKa = 4.0TVLLGQPIGPEE214 pKa = 4.23FWAARR219 pKa = 11.84HH220 pKa = 5.66GADD223 pKa = 4.04FCAEE227 pKa = 3.86

Molecular weight: 24.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081CMQ8|A0A081CMQ8_PSEA2 D-ribose-5-phosphate ketol-isomerase OS=Pseudozyma antarctica OX=84753 GN=PAN0_028c6184 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 10.05RR3 pKa = 11.84KK4 pKa = 9.37KK5 pKa = 9.97MKK7 pKa = 8.83MKK9 pKa = 10.77LMILMMVTLLLLLQCNDD26 pKa = 3.33QPEE29 pKa = 4.26LADD32 pKa = 3.67RR33 pKa = 11.84VSVVPVLSICDD44 pKa = 3.98ALEE47 pKa = 4.1HH48 pKa = 6.62AATSLPHH55 pKa = 6.01KK56 pKa = 9.58HH57 pKa = 6.1AKK59 pKa = 7.69KK60 pKa = 8.9TLRR63 pKa = 11.84LDD65 pKa = 3.4RR66 pKa = 11.84KK67 pKa = 8.49TPLPCCAAALLPPALLRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84SASLILARR94 pKa = 11.84SGSSLLGVDD103 pKa = 3.49HH104 pKa = 7.21RR105 pKa = 11.84LVISPSSTAAQAACAPARR123 pKa = 11.84AALHH127 pKa = 5.85LAHH130 pKa = 7.18RR131 pKa = 11.84PLLASFPAPVVRR143 pKa = 11.84ISARR147 pKa = 11.84DD148 pKa = 3.45GLDD151 pKa = 3.29PLTASVSLEE160 pKa = 3.69SRR162 pKa = 11.84RR163 pKa = 11.84FRR165 pKa = 11.84PYY167 pKa = 9.67MRR169 pKa = 11.84EE170 pKa = 3.29SCYY173 pKa = 10.46RR174 pKa = 11.84IARR177 pKa = 11.84RR178 pKa = 11.84SSGPYY183 pKa = 9.44QISEE187 pKa = 4.16VSLAWSFASNDD198 pKa = 3.59TSLSTPACLLRR209 pKa = 11.84AFPYY213 pKa = 9.08STSSLLVISSVTKK226 pKa = 10.61RR227 pKa = 11.84SS228 pKa = 3.2

Molecular weight: 24.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4672

0

4672

2859900

33

5427

612.1

66.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.786 ± 0.042

0.989 ± 0.01

5.804 ± 0.028

5.339 ± 0.027

3.152 ± 0.017

6.777 ± 0.03

2.643 ± 0.016

3.781 ± 0.023

4.104 ± 0.028

8.834 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.931 ± 0.01

2.992 ± 0.017

6.468 ± 0.038

4.198 ± 0.03

6.708 ± 0.027

9.532 ± 0.053

5.737 ± 0.022

5.877 ± 0.027

1.192 ± 0.012

2.153 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski