Influenza B virus (strain B/Singapore/222/1979)
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P12600|NS1_INBSI Non-structural protein 1 OS=Influenza B virus (strain B/Singapore/222/1979) OX=107417 GN=NS PE=3 SV=1
MM1 pKa = 7.72 DD2 pKa = 4.03 TFITRR7 pKa = 11.84 NFQTTIIQKK16 pKa = 10.29 AKK18 pKa = 8.58 NTMAEE23 pKa = 4.07 FMEE26 pKa = 5.03 DD27 pKa = 3.9 PEE29 pKa = 4.82 LQPAMLFNICVHH41 pKa = 6.68 LEE43 pKa = 3.78 VCYY46 pKa = 10.78 VISDD50 pKa = 3.68 MNFLDD55 pKa = 4.43 EE56 pKa = 4.43 EE57 pKa = 4.77 GKK59 pKa = 10.67 AYY61 pKa = 8.15 TALEE65 pKa = 4.33 GQGKK69 pKa = 7.68 EE70 pKa = 3.94 QNLRR74 pKa = 11.84 PQYY77 pKa = 10.54 EE78 pKa = 4.57 VIEE81 pKa = 4.19 GMPRR85 pKa = 11.84 NIAWMVQRR93 pKa = 11.84 SLAQEE98 pKa = 4.19 HH99 pKa = 6.59 GIEE102 pKa = 4.28 TPKK105 pKa = 10.97 YY106 pKa = 9.38 LADD109 pKa = 3.81 LFDD112 pKa = 4.22 YY113 pKa = 8.3 KK114 pKa = 9.99 TKK116 pKa = 10.82 RR117 pKa = 11.84 FIEE120 pKa = 4.0 VGITKK125 pKa = 10.32 GLADD129 pKa = 4.42 DD130 pKa = 4.63 YY131 pKa = 11.19 FWKK134 pKa = 10.59 KK135 pKa = 10.28 KK136 pKa = 9.87 EE137 pKa = 3.82 KK138 pKa = 10.49 LGNSMEE144 pKa = 4.41 LMIFSYY150 pKa = 10.74 NQDD153 pKa = 2.93 YY154 pKa = 11.2 SLSNEE159 pKa = 3.9 SSLDD163 pKa = 3.56 EE164 pKa = 4.4 EE165 pKa = 4.86 GKK167 pKa = 10.97 GRR169 pKa = 11.84 VLSRR173 pKa = 11.84 LTEE176 pKa = 4.09 LQAEE180 pKa = 4.6 LSLKK184 pKa = 10.43 NLWQVLIGEE193 pKa = 4.28 EE194 pKa = 4.54 DD195 pKa = 3.28 IEE197 pKa = 4.69 KK198 pKa = 11.15 GIDD201 pKa = 3.45 FKK203 pKa = 11.36 LGQTISRR210 pKa = 11.84 LRR212 pKa = 11.84 DD213 pKa = 3.1 ISVPAGFSNFEE224 pKa = 4.0 GMRR227 pKa = 11.84 SYY229 pKa = 11.04 IDD231 pKa = 4.25 NIDD234 pKa = 3.38 PKK236 pKa = 11.14 GAIEE240 pKa = 4.13 RR241 pKa = 11.84 NLARR245 pKa = 11.84 MSPLVSVTPKK255 pKa = 9.85 KK256 pKa = 10.83 LKK258 pKa = 10.23 WEE260 pKa = 4.3 DD261 pKa = 3.41 LRR263 pKa = 11.84 PIGPHH268 pKa = 6.43 IYY270 pKa = 9.79 NHH272 pKa = 6.28 EE273 pKa = 4.17 LPEE276 pKa = 4.04 VPYY279 pKa = 10.96 NAFLLMSDD287 pKa = 3.99 EE288 pKa = 4.95 LGLANMTEE296 pKa = 4.57 GKK298 pKa = 9.69 SKK300 pKa = 10.66 KK301 pKa = 9.72 PKK303 pKa = 9.48 TLAKK307 pKa = 10.0 EE308 pKa = 4.07 CLEE311 pKa = 4.39 KK312 pKa = 11.23 YY313 pKa = 8.63 STLRR317 pKa = 11.84 DD318 pKa = 3.36 QTDD321 pKa = 4.33 PILIMKK327 pKa = 9.41 SEE329 pKa = 4.03 KK330 pKa = 10.78 ANEE333 pKa = 3.8 NFLWKK338 pKa = 10.42 LWRR341 pKa = 11.84 DD342 pKa = 3.76 CVNTISNEE350 pKa = 3.98 EE351 pKa = 4.1 TSNEE355 pKa = 3.91 LQKK358 pKa = 10.16 TNYY361 pKa = 9.82 AKK363 pKa = 10.3 WATGDD368 pKa = 3.24 GLTYY372 pKa = 10.2 QKK374 pKa = 10.63 IMKK377 pKa = 9.33 EE378 pKa = 3.96 VAIDD382 pKa = 3.9 DD383 pKa = 3.68 EE384 pKa = 5.15 TMCQEE389 pKa = 4.25 EE390 pKa = 4.1 PKK392 pKa = 10.31 IPNKK396 pKa = 10.07 CRR398 pKa = 11.84 VAAWVQTEE406 pKa = 4.14 MNLLSTLTSKK416 pKa = 10.85 RR417 pKa = 11.84 ALDD420 pKa = 3.91 LPEE423 pKa = 5.27 IGPDD427 pKa = 3.43 VAPVEE432 pKa = 4.56 HH433 pKa = 6.53 VGSEE437 pKa = 3.58 RR438 pKa = 11.84 RR439 pKa = 11.84 KK440 pKa = 9.81 YY441 pKa = 9.86 FVNEE445 pKa = 3.34 INYY448 pKa = 9.25 CKK450 pKa = 10.56 ASTVMMKK457 pKa = 10.46 YY458 pKa = 10.74 VLFHH462 pKa = 6.42 TSLLNEE468 pKa = 4.68 SNASMGKK475 pKa = 9.99 YY476 pKa = 9.62 KK477 pKa = 10.59 VIPITNRR484 pKa = 11.84 VNEE487 pKa = 4.2 KK488 pKa = 10.96 GEE490 pKa = 4.3 SFDD493 pKa = 3.5 MLYY496 pKa = 11.24 GLAVKK501 pKa = 9.91 GQSHH505 pKa = 6.71 LRR507 pKa = 11.84 GDD509 pKa = 3.87 TDD511 pKa = 3.58 VVTVVTFEE519 pKa = 4.42 FSSTDD524 pKa = 3.3 PRR526 pKa = 11.84 VDD528 pKa = 3.05 SGKK531 pKa = 8.47 WPKK534 pKa = 9.4 YY535 pKa = 6.84 TVFRR539 pKa = 11.84 IGSLFVSGRR548 pKa = 11.84 EE549 pKa = 3.75 KK550 pKa = 10.61 SVYY553 pKa = 10.09 LYY555 pKa = 11.01 CRR557 pKa = 11.84 VNGTNKK563 pKa = 9.61 IQMKK567 pKa = 8.84 WGMEE571 pKa = 3.68 ARR573 pKa = 11.84 RR574 pKa = 11.84 CLLQSMQQMEE584 pKa = 5.02 AIVEE588 pKa = 4.22 QEE590 pKa = 4.1 SSIQGYY596 pKa = 11.18 DD597 pKa = 3.05 MTKK600 pKa = 10.49 ACFKK604 pKa = 9.93 EE605 pKa = 4.19 DD606 pKa = 3.17 RR607 pKa = 11.84 VNSPKK612 pKa = 10.09 TFSIGTQEE620 pKa = 3.93 GKK622 pKa = 10.13 LVKK625 pKa = 10.6 GSFGKK630 pKa = 10.38 ALRR633 pKa = 11.84 VIFTKK638 pKa = 10.82 CLMHH642 pKa = 6.24 YY643 pKa = 10.55 VFGNAQLEE651 pKa = 4.61 GFSAEE656 pKa = 4.01 SRR658 pKa = 11.84 RR659 pKa = 11.84 LLLLIQALKK668 pKa = 10.37 DD669 pKa = 3.68 RR670 pKa = 11.84 KK671 pKa = 10.3 GPWVFDD677 pKa = 3.85 LEE679 pKa = 4.38 GMYY682 pKa = 10.41 SGIEE686 pKa = 3.85 EE687 pKa = 4.9 CISNNPWVIQSAYY700 pKa = 8.73 WFNEE704 pKa = 3.19 WLGFEE709 pKa = 4.34 KK710 pKa = 10.6 EE711 pKa = 4.12 GSKK714 pKa = 10.62 VLEE717 pKa = 4.4 SVDD720 pKa = 4.31 EE721 pKa = 4.55 IMDD724 pKa = 3.72 EE725 pKa = 4.12
Molecular weight: 83.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.302
IPC2_protein 5.41
IPC_protein 5.372
Toseland 5.347
ProMoST 5.461
Dawson 5.372
Bjellqvist 5.499
Wikipedia 5.232
Rodwell 5.308
Grimsley 5.283
Solomon 5.372
Lehninger 5.334
Nozaki 5.499
DTASelect 5.614
Thurlkill 5.372
EMBOSS 5.296
Sillero 5.588
Patrickios 4.228
IPC_peptide 5.385
IPC2_peptide 5.575
IPC2.peptide.svr19 5.627
Protein with the highest isoelectric point:
>tr|Q9QLL3|Q9QLL3_INBSI Polymerase basic protein 2 OS=Influenza B virus (strain B/Singapore/222/1979) OX=107417 GN=PB2 PE=3 SV=1
MM1 pKa = 7.2 TLAKK5 pKa = 10.05 IEE7 pKa = 4.27 LLKK10 pKa = 10.82 QLLRR14 pKa = 11.84 DD15 pKa = 3.91 NEE17 pKa = 4.23 AKK19 pKa = 9.7 TVLKK23 pKa = 9.15 QTTVDD28 pKa = 3.07 QYY30 pKa = 11.86 NIIRR34 pKa = 11.84 KK35 pKa = 8.85 FNTSRR40 pKa = 11.84 IEE42 pKa = 4.0 KK43 pKa = 10.24 NPSLRR48 pKa = 11.84 MKK50 pKa = 9.92 WAMCSNFPLALTKK63 pKa = 10.87 GDD65 pKa = 3.51 MANRR69 pKa = 11.84 IPLEE73 pKa = 3.92 YY74 pKa = 10.33 KK75 pKa = 10.63 GIQLKK80 pKa = 9.38 TNAEE84 pKa = 4.42 DD85 pKa = 3.46 IGTKK89 pKa = 10.43 GQMCSIAAVTWWNTYY104 pKa = 10.1 GPIGDD109 pKa = 3.74 TEE111 pKa = 4.35 GFEE114 pKa = 4.22 KK115 pKa = 10.73 VYY117 pKa = 11.02 EE118 pKa = 4.3 SFFLRR123 pKa = 11.84 KK124 pKa = 8.78 MRR126 pKa = 11.84 LDD128 pKa = 3.16 NATWGRR134 pKa = 11.84 ITFGPVEE141 pKa = 4.17 RR142 pKa = 11.84 VRR144 pKa = 11.84 KK145 pKa = 9.27 RR146 pKa = 11.84 VLLNPLTKK154 pKa = 10.41 EE155 pKa = 3.99 MPPDD159 pKa = 3.45 EE160 pKa = 4.69 ASNVIMEE167 pKa = 4.24 ILFPKK172 pKa = 9.72 EE173 pKa = 3.27 AGIPRR178 pKa = 11.84 EE179 pKa = 4.2 STWIHH184 pKa = 6.43 RR185 pKa = 11.84 EE186 pKa = 3.8 LIKK189 pKa = 10.54 EE190 pKa = 3.96 KK191 pKa = 10.54 RR192 pKa = 11.84 EE193 pKa = 3.94 KK194 pKa = 11.07 LKK196 pKa = 10.21 GTMITPIVLAYY207 pKa = 8.9 MLEE210 pKa = 4.26 RR211 pKa = 11.84 EE212 pKa = 4.28 LVARR216 pKa = 11.84 RR217 pKa = 11.84 RR218 pKa = 11.84 FLPVAGATSAEE229 pKa = 4.51 FIEE232 pKa = 4.74 MLHH235 pKa = 6.25 CLQGEE240 pKa = 4.23 NWRR243 pKa = 11.84 QIYY246 pKa = 10.2 HH247 pKa = 6.78 PGGNKK252 pKa = 8.44 LTEE255 pKa = 4.17 SRR257 pKa = 11.84 SQSMIVACRR266 pKa = 11.84 KK267 pKa = 9.17 IIRR270 pKa = 11.84 RR271 pKa = 11.84 SIVASNPLEE280 pKa = 4.1 LAVEE284 pKa = 4.58 IANKK288 pKa = 8.86 TVIDD292 pKa = 3.95 TEE294 pKa = 4.23 PLKK297 pKa = 11.19 SCLAAIDD304 pKa = 4.49 GGDD307 pKa = 3.66 VACDD311 pKa = 3.12 IMRR314 pKa = 11.84 AALGLKK320 pKa = 9.13 IRR322 pKa = 11.84 QRR324 pKa = 11.84 QRR326 pKa = 11.84 FGRR329 pKa = 11.84 LEE331 pKa = 4.07 LKK333 pKa = 10.28 RR334 pKa = 11.84 ISGRR338 pKa = 11.84 GFKK341 pKa = 10.29 NDD343 pKa = 3.3 EE344 pKa = 4.59 EE345 pKa = 4.2 ILIGNGTIQKK355 pKa = 10.0 IGIWDD360 pKa = 3.73 GEE362 pKa = 4.47 EE363 pKa = 3.99 EE364 pKa = 4.11 FHH366 pKa = 6.94 VRR368 pKa = 11.84 CGEE371 pKa = 3.88 CRR373 pKa = 11.84 GILKK377 pKa = 9.91 KK378 pKa = 10.83 SKK380 pKa = 9.48 MRR382 pKa = 11.84 MEE384 pKa = 4.32 KK385 pKa = 10.82 LLINSAKK392 pKa = 10.33 KK393 pKa = 10.58 EE394 pKa = 3.99 DD395 pKa = 3.88 MKK397 pKa = 11.48 DD398 pKa = 3.71 LIILCMVFSQDD409 pKa = 2.31 TRR411 pKa = 11.84 MFQGVRR417 pKa = 11.84 GEE419 pKa = 4.12 INFLNRR425 pKa = 11.84 AGQLLSPMYY434 pKa = 10.01 QLQRR438 pKa = 11.84 YY439 pKa = 7.37 FLNRR443 pKa = 11.84 SNDD446 pKa = 3.9 LFDD449 pKa = 2.96 QWGYY453 pKa = 10.98 EE454 pKa = 4.08 EE455 pKa = 4.74 SPKK458 pKa = 10.73 ASEE461 pKa = 3.57 LHH463 pKa = 6.88 GINEE467 pKa = 4.33 LMNASDD473 pKa = 3.92 YY474 pKa = 8.88 TLKK477 pKa = 11.05 GVVVTKK483 pKa = 10.76 NVIDD487 pKa = 4.64 DD488 pKa = 4.65 FSSTEE493 pKa = 4.04 TEE495 pKa = 4.03 KK496 pKa = 11.31 VSITKK501 pKa = 10.01 NLSLIKK507 pKa = 9.88 RR508 pKa = 11.84 TGEE511 pKa = 4.12 VIMGANDD518 pKa = 3.09 ISEE521 pKa = 5.01 LEE523 pKa = 4.23 SQAQLMITYY532 pKa = 7.68 DD533 pKa = 3.87 TPKK536 pKa = 8.17 MWEE539 pKa = 3.77 MGTTKK544 pKa = 10.43 EE545 pKa = 4.29 LVQNTYY551 pKa = 8.21 QWVLKK556 pKa = 10.91 NLVTLKK562 pKa = 10.93 AQFLLGKK569 pKa = 9.96 EE570 pKa = 5.88 DD571 pKa = 3.54 MFQWDD576 pKa = 3.33 AFEE579 pKa = 4.66 AFEE582 pKa = 5.18 SIIPQKK588 pKa = 10.32 MAGQYY593 pKa = 10.54 SGFARR598 pKa = 11.84 AVLKK602 pKa = 9.91 QMRR605 pKa = 11.84 DD606 pKa = 3.47 QEE608 pKa = 4.51 VMKK611 pKa = 10.1 TDD613 pKa = 3.47 QFIKK617 pKa = 10.67 LLPFCFSPPKK627 pKa = 10.06 LRR629 pKa = 11.84 SNGEE633 pKa = 3.67 PYY635 pKa = 10.49 QFLRR639 pKa = 11.84 LILKK643 pKa = 10.43 GGGEE647 pKa = 4.11 NFIEE651 pKa = 4.26 VRR653 pKa = 11.84 KK654 pKa = 10.13 GSPLFSYY661 pKa = 10.68 NPQTEE666 pKa = 4.12 VLTICGRR673 pKa = 11.84 MMSLKK678 pKa = 10.66 GKK680 pKa = 10.2 IEE682 pKa = 4.01 DD683 pKa = 3.72 EE684 pKa = 3.95 EE685 pKa = 5.12 RR686 pKa = 11.84 NRR688 pKa = 11.84 SMGNAVLAGFLVSGKK703 pKa = 10.1 YY704 pKa = 10.53 DD705 pKa = 3.9 PDD707 pKa = 5.06 LGDD710 pKa = 3.6 FKK712 pKa = 10.87 TIEE715 pKa = 4.29 EE716 pKa = 4.4 LEE718 pKa = 4.1 KK719 pKa = 11.0 LKK721 pKa = 10.75 PGEE724 pKa = 3.97 KK725 pKa = 10.57 ANILLYY731 pKa = 10.07 QGKK734 pKa = 7.97 PVKK737 pKa = 9.66 VVKK740 pKa = 10.37 RR741 pKa = 11.84 KK742 pKa = 9.92 RR743 pKa = 11.84 YY744 pKa = 8.87 SALSNDD750 pKa = 2.67 ISQGIKK756 pKa = 8.29 RR757 pKa = 11.84 QRR759 pKa = 11.84 MTVEE763 pKa = 3.77 SMGWALSS770 pKa = 3.61
Molecular weight: 87.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.691
IPC2_protein 8.507
IPC_protein 8.375
Toseland 9.238
ProMoST 8.99
Dawson 9.502
Bjellqvist 9.268
Wikipedia 9.619
Rodwell 9.853
Grimsley 9.531
Solomon 9.531
Lehninger 9.502
Nozaki 9.472
DTASelect 9.18
Thurlkill 9.385
EMBOSS 9.692
Sillero 9.502
Patrickios 5.245
IPC_peptide 9.531
IPC2_peptide 7.805
IPC2.peptide.svr19 7.774
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
4034
100
770
448.2
50.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.049 ± 0.429
2.256 ± 0.339
4.611 ± 0.268
7.313 ± 0.742
3.718 ± 0.296
7.362 ± 0.439
1.71 ± 0.309
6.619 ± 0.419
7.536 ± 0.368
9.147 ± 0.421
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.743 ± 0.407
5.429 ± 0.593
4.09 ± 0.359
3.074 ± 0.396
5.181 ± 0.408
6.544 ± 0.392
6.197 ± 0.53
5.231 ± 0.329
1.264 ± 0.219
2.925 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here