Chitinophaga sp. YR573
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6483 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I0RVF3|A0A1I0RVF3_9BACT Uncharacterized protein OS=Chitinophaga sp. YR573 OX=1881040 GN=SAMN05428988_6014 PE=4 SV=1
MM1 pKa = 7.34 YY2 pKa = 10.9 NLTTSTALMKK12 pKa = 10.08 NQKK15 pKa = 9.33 AAQVLSDD22 pKa = 3.35 QSLFSVLQNSQGQSLFFSIGDD43 pKa = 3.69 DD44 pKa = 4.04 AILYY48 pKa = 10.1 LSAEE52 pKa = 4.2 QDD54 pKa = 3.22 NATTGWTPIDD64 pKa = 3.96 LTTEE68 pKa = 4.2 LAADD72 pKa = 3.83 YY73 pKa = 9.62 TGKK76 pKa = 8.3 TVTAKK81 pKa = 10.07 TFVVSQDD88 pKa = 2.88 ATTGDD93 pKa = 3.7 ILILQAIHH101 pKa = 5.84 VAEE104 pKa = 4.99 DD105 pKa = 3.39 NADD108 pKa = 3.72 YY109 pKa = 11.1 LYY111 pKa = 10.42 MLSGLSNAPDD121 pKa = 4.09 AAWLTASSNRR131 pKa = 11.84 SWTARR136 pKa = 11.84 LYY138 pKa = 11.11 DD139 pKa = 3.75 DD140 pKa = 4.04 TADD143 pKa = 3.75 PVSNTDD149 pKa = 2.7 IAYY152 pKa = 9.82 INIALNQDD160 pKa = 4.84 PIQAPYY166 pKa = 10.4 VIVGLQDD173 pKa = 3.09 QATTFIQNYY182 pKa = 7.77 VVSIDD187 pKa = 3.46 PTTTTGIWTKK197 pKa = 11.13 YY198 pKa = 6.48 EE199 pKa = 3.71 TAEE202 pKa = 5.11 NYY204 pKa = 8.99 DD205 pKa = 3.48 QLYY208 pKa = 10.76 GMAIGKK214 pKa = 8.81 AQAAMFAGLYY224 pKa = 9.47 EE225 pKa = 5.39 LYY227 pKa = 9.95 TLNDD231 pKa = 3.45 NTSLTFTPLKK241 pKa = 10.72 SVFGPPDD248 pKa = 4.0 IIKK251 pKa = 8.46 LTPPDD256 pKa = 3.8 GATAIATMPPDD267 pKa = 3.35 SSGNTNLYY275 pKa = 9.66 VAGTGAIYY283 pKa = 10.45 LFSPDD288 pKa = 3.37 QQLNDD293 pKa = 3.48 ATGTAVITNDD303 pKa = 3.94 LIVGVEE309 pKa = 4.04 YY310 pKa = 10.5 FKK312 pKa = 11.2 VHH314 pKa = 5.51 QSGNQVTLWGKK325 pKa = 10.27 DD326 pKa = 3.2 NEE328 pKa = 4.49 GTVFYY333 pKa = 10.91 SRR335 pKa = 11.84 CDD337 pKa = 3.13 VDD339 pKa = 4.16 NQTDD343 pKa = 3.82 PGSWSCPLPLLVQVEE358 pKa = 4.35 QLASLLDD365 pKa = 3.5 IQTQSSVLYY374 pKa = 10.58 AHH376 pKa = 6.25 TTGQSLVKK384 pKa = 10.36 LIQDD388 pKa = 3.44 AVTTQWSSQSILLPALSTSDD408 pKa = 2.71 IYY410 pKa = 11.05 EE411 pKa = 4.11 YY412 pKa = 11.45 YY413 pKa = 11.19 AFTTTLKK420 pKa = 9.66 VTDD423 pKa = 4.97 DD424 pKa = 3.75 NNLPIGGQAVQITATSPCSLYY445 pKa = 10.02 MDD447 pKa = 3.84 NQYY450 pKa = 8.53 VTLLPDD456 pKa = 3.22 VPLTVTSDD464 pKa = 3.36 STGMITFVQEE474 pKa = 4.12 TQTLGAVCYY483 pKa = 10.36 NFQQDD488 pKa = 3.68 DD489 pKa = 4.24 NSWLNVNPMNNIVQSMSGITQGSDD513 pKa = 2.71 LTAVNVTDD521 pKa = 4.35 EE522 pKa = 4.53 YY523 pKa = 11.26 GTTTPLVPSTIPADD537 pKa = 3.65 QADD540 pKa = 3.96 AVAKK544 pKa = 10.62 GIAQFVSTAQTLPQDD559 pKa = 3.88 GSLQSTGSFAASEE572 pKa = 4.19 GLVWGLSFANNSIQYY587 pKa = 10.14 FEE589 pKa = 5.29 GSSQYY594 pKa = 10.91 AAAGIIPASDD604 pKa = 2.8 IGNAIEE610 pKa = 5.38 AFAGDD615 pKa = 3.26 IFKK618 pKa = 10.22 WLEE621 pKa = 3.74 NAVDD625 pKa = 3.97 EE626 pKa = 4.58 VAQFVVKK633 pKa = 10.89 VIDD636 pKa = 4.08 GVTHH640 pKa = 6.08 FFIEE644 pKa = 4.03 IGDD647 pKa = 3.97 AVYY650 pKa = 10.55 HH651 pKa = 7.08 FIMQCINDD659 pKa = 4.0 VVHH662 pKa = 7.28 GIQFILSKK670 pKa = 10.57 IEE672 pKa = 3.88 AGFEE676 pKa = 4.39 KK677 pKa = 10.46 LVQWVGFIFSWSDD690 pKa = 3.15 IIRR693 pKa = 11.84 THH695 pKa = 6.22 NVLKK699 pKa = 10.95 NIFAQYY705 pKa = 10.42 SQYY708 pKa = 11.06 CVDD711 pKa = 4.6 NISSYY716 pKa = 10.96 KK717 pKa = 8.69 QTLLDD722 pKa = 3.57 TLSTINEE729 pKa = 4.93 EE730 pKa = 3.5 IDD732 pKa = 2.93 QWANIPAVSDD742 pKa = 4.08 TIAGSNASSPSMPGKK757 pKa = 10.22 DD758 pKa = 3.13 SPQSHH763 pKa = 6.26 WALYY767 pKa = 7.29 HH768 pKa = 5.43 TKK770 pKa = 10.61 GNVAGASTTFDD781 pKa = 3.0 ASFDD785 pKa = 3.73 GDD787 pKa = 3.96 ALSLIIADD795 pKa = 4.3 LCTALEE801 pKa = 4.06 NEE803 pKa = 4.36 EE804 pKa = 4.34 QNFEE808 pKa = 4.29 DD809 pKa = 4.36 VLAALKK815 pKa = 9.98 TDD817 pKa = 3.92 VIDD820 pKa = 4.69 QIGTLSASEE829 pKa = 5.27 LITNLLGIIANLLIDD844 pKa = 3.76 TVGNIIGTVMDD855 pKa = 4.99 IIAALVAGVVEE866 pKa = 4.88 ALTTPLDD873 pKa = 3.61 IPVISYY879 pKa = 9.95 IYY881 pKa = 11.06 NKK883 pKa = 9.13 FTGNDD888 pKa = 3.4 LTLLDD893 pKa = 4.42 LTCLIVSIPVTIIYY907 pKa = 10.34 KK908 pKa = 10.04 LIKK911 pKa = 9.86 EE912 pKa = 4.34 ATPFPDD918 pKa = 5.1 DD919 pKa = 4.67 SFTQSLIAATDD930 pKa = 3.38 YY931 pKa = 7.9 TTIQNLYY938 pKa = 10.21 AGASSLAVADD948 pKa = 4.64 DD949 pKa = 4.69 DD950 pKa = 5.11 GVTPVQIITLTLAISGYY967 pKa = 9.46 FGSIAMIIMTAVKK980 pKa = 10.04 AAAPQSAAVCTVYY993 pKa = 11.24 ALAYY997 pKa = 9.97 LPYY1000 pKa = 9.39 IAPNIAITKK1009 pKa = 9.62 PSTQKK1014 pKa = 10.07 WYY1016 pKa = 11.1 VDD1018 pKa = 3.25 MDD1020 pKa = 4.23 EE1021 pKa = 5.13 IITVISVIKK1030 pKa = 9.26 TFSDD1034 pKa = 2.82 ITLYY1038 pKa = 10.59 KK1039 pKa = 9.84 YY1040 pKa = 10.91 DD1041 pKa = 4.28 KK1042 pKa = 10.34 NDD1044 pKa = 3.67 PEE1046 pKa = 5.13 ASQTLAKK1053 pKa = 8.61 WAKK1056 pKa = 8.62 ASPYY1060 pKa = 10.32 VEE1062 pKa = 4.91 LLINVIWEE1070 pKa = 3.85 IPTIGFIADD1079 pKa = 3.5 NQDD1082 pKa = 2.74 AGTVLGFLGNTSFNVSGILSPWSDD1106 pKa = 2.98 TDD1108 pKa = 3.7 YY1109 pKa = 11.88 VMLGIVIFIGLYY1121 pKa = 9.81 GEE1123 pKa = 4.2 MMLVQGMVNFPAPSSAKK1140 pKa = 10.15 YY1141 pKa = 10.12 LATKK1145 pKa = 9.51 EE1146 pKa = 4.02 AA1147 pKa = 3.95
Molecular weight: 123.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.783
IPC_protein 3.846
Toseland 3.605
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.503
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.228
Thurlkill 3.656
EMBOSS 3.795
Sillero 3.961
Patrickios 0.439
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.845
Protein with the highest isoelectric point:
>tr|A0A1I0Q2Q1|A0A1I0Q2Q1_9BACT Uncharacterized protein OS=Chitinophaga sp. YR573 OX=1881040 GN=SAMN05428988_3166 PE=4 SV=1
MM1 pKa = 6.41 NTKK4 pKa = 9.41 IRR6 pKa = 11.84 AFIIMLLLYY15 pKa = 9.7 GLHH18 pKa = 6.08 VYY20 pKa = 10.76 LMNTLLSIHH29 pKa = 6.78 SNMSDD34 pKa = 2.46 ILAFLMMIACPFYY47 pKa = 10.97 KK48 pKa = 9.84 RR49 pKa = 11.84 IHH51 pKa = 5.53 QRR53 pKa = 11.84 LVATLKK59 pKa = 10.66 NRR61 pKa = 3.55
Molecular weight: 7.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.955
IPC_protein 10.526
Toseland 10.379
ProMoST 10.467
Dawson 10.57
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.891
Grimsley 10.657
Solomon 10.628
Lehninger 10.599
Nozaki 10.379
DTASelect 10.277
Thurlkill 10.423
EMBOSS 10.774
Sillero 10.482
Patrickios 10.716
IPC_peptide 10.628
IPC2_peptide 9.326
IPC2.peptide.svr19 8.431
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6483
0
6483
2436871
29
7223
375.9
42.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.613 ± 0.033
0.826 ± 0.011
5.334 ± 0.021
5.302 ± 0.036
4.577 ± 0.021
6.936 ± 0.029
1.92 ± 0.015
7.016 ± 0.027
6.352 ± 0.029
9.389 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.337 ± 0.016
5.499 ± 0.029
4.023 ± 0.021
3.89 ± 0.021
4.007 ± 0.022
6.466 ± 0.031
6.428 ± 0.058
6.436 ± 0.028
1.297 ± 0.013
4.352 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here