Wisteria badnavirus 1

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9IRR3|A0A1U9IRR3_9VIRU ORF2 OS=Wisteria badnavirus 1 OX=1973265 PE=4 SV=1
MM1 pKa = 7.57SRR3 pKa = 11.84EE4 pKa = 3.62EE5 pKa = 4.19DD6 pKa = 3.36YY7 pKa = 11.33KK8 pKa = 11.61AFLEE12 pKa = 4.39EE13 pKa = 4.42WNPDD17 pKa = 3.82PKK19 pKa = 11.02DD20 pKa = 3.55KK21 pKa = 10.79EE22 pKa = 4.77ANFLDD27 pKa = 4.59SILPEE32 pKa = 3.81PHH34 pKa = 6.65HH35 pKa = 6.81LNRR38 pKa = 11.84HH39 pKa = 5.38YY40 pKa = 11.53YY41 pKa = 10.08NLPCFHH47 pKa = 7.11LEE49 pKa = 4.21STAHH53 pKa = 5.2NHH55 pKa = 5.8SLGKK59 pKa = 10.23KK60 pKa = 8.99SSEE63 pKa = 4.09PTTSSQLDD71 pKa = 3.56LLLTLVHH78 pKa = 6.18NQSIHH83 pKa = 5.77YY84 pKa = 9.82DD85 pKa = 3.18RR86 pKa = 11.84TIRR89 pKa = 11.84FHH91 pKa = 6.34QAIFSQLEE99 pKa = 4.13RR100 pKa = 11.84IEE102 pKa = 4.33TRR104 pKa = 11.84LTTLEE109 pKa = 3.75ARR111 pKa = 11.84QPPTSNTNQTQTPKK125 pKa = 10.48PPRR128 pKa = 11.84KK129 pKa = 9.53EE130 pKa = 3.85YY131 pKa = 11.06LDD133 pKa = 3.56LFQRR137 pKa = 11.84LEE139 pKa = 4.07DD140 pKa = 3.5RR141 pKa = 11.84LKK143 pKa = 10.92AIEE146 pKa = 4.77DD147 pKa = 4.21NIPSS151 pKa = 3.5

Molecular weight: 17.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9IRR4|A0A1U9IRR4_9VIRU Reverse transcriptase OS=Wisteria badnavirus 1 OX=1973265 PE=4 SV=1
MM1 pKa = 7.42SSWISSSEE9 pKa = 3.78TSVYY13 pKa = 10.83KK14 pKa = 10.01DD15 pKa = 3.01TLEE18 pKa = 4.29AVSTLSGDD26 pKa = 3.63HH27 pKa = 6.57EE28 pKa = 4.86GGEE31 pKa = 4.58GFVKK35 pKa = 10.45PDD37 pKa = 3.41TLGSKK42 pKa = 10.83GITHH46 pKa = 6.84CVKK49 pKa = 10.56QNNFQIQILVQITKK63 pKa = 10.3RR64 pKa = 11.84LEE66 pKa = 4.65DD67 pKa = 3.37IQLLLEE73 pKa = 4.35KK74 pKa = 10.51QGEE77 pKa = 4.39RR78 pKa = 11.84INALEE83 pKa = 4.52KK84 pKa = 10.38EE85 pKa = 4.57VKK87 pKa = 8.67TLKK90 pKa = 9.78TADD93 pKa = 3.71FKK95 pKa = 11.65VPEE98 pKa = 4.53DD99 pKa = 4.49VIDD102 pKa = 4.62DD103 pKa = 3.92LTKK106 pKa = 10.62KK107 pKa = 10.12FAAIEE112 pKa = 3.9TSALKK117 pKa = 10.21KK118 pKa = 10.5AKK120 pKa = 10.26QKK122 pKa = 8.17VTKK125 pKa = 9.72EE126 pKa = 3.66KK127 pKa = 9.99TPFLIHH133 pKa = 6.91HH134 pKa = 7.26DD135 pKa = 3.88PYY137 pKa = 11.36EE138 pKa = 4.13LNKK141 pKa = 10.47SIKK144 pKa = 9.92RR145 pKa = 3.7

Molecular weight: 16.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2357

145

1909

589.3

66.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.152 ± 0.61

1.782 ± 0.334

5.049 ± 0.33

8.316 ± 0.329

3.649 ± 0.093

6.279 ± 1.34

2.164 ± 0.981

6.406 ± 0.494

6.831 ± 1.256

8.188 ± 1.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.459

4.54 ± 0.396

4.922 ± 0.716

5.6 ± 0.245

5.643 ± 0.737

6.406 ± 0.522

6.067 ± 1.368

5.431 ± 1.398

1.358 ± 0.474

3.012 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski