Acinetobacter phage WCHABP12
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V0DZ59|A0A1V0DZ59_9CAUD Uncharacterized protein OS=Acinetobacter phage WCHABP12 OX=1965454 GN=ABP12_00007 PE=4 SV=1
MM1 pKa = 7.25 AQYY4 pKa = 9.85 DD5 pKa = 3.73 IPLAQVPNQFFTTSLNGVTWAITLEE30 pKa = 4.08 TRR32 pKa = 11.84 LNNLYY37 pKa = 10.27 ISLSNNNDD45 pKa = 2.72 GDD47 pKa = 3.93 VLLNRR52 pKa = 11.84 ICLNRR57 pKa = 11.84 TYY59 pKa = 10.23 LGHH62 pKa = 6.75 GFIFVDD68 pKa = 3.51 IDD70 pKa = 3.79 GNSDD74 pKa = 3.53 PEE76 pKa = 4.25 YY77 pKa = 9.62 TGLGTRR83 pKa = 11.84 YY84 pKa = 10.14 LLIWTDD90 pKa = 3.71 EE91 pKa = 4.13 VV92 pKa = 3.51
Molecular weight: 10.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.887
IPC2_protein 4.151
IPC_protein 4.037
Toseland 3.834
ProMoST 4.215
Dawson 4.037
Bjellqvist 4.202
Wikipedia 3.999
Rodwell 3.872
Grimsley 3.745
Solomon 4.024
Lehninger 3.973
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.91
EMBOSS 4.012
Sillero 4.164
Patrickios 0.782
IPC_peptide 4.012
IPC2_peptide 4.139
IPC2.peptide.svr19 4.04
Protein with the highest isoelectric point:
>tr|A0A1V0DZA9|A0A1V0DZA9_9CAUD Uncharacterized protein OS=Acinetobacter phage WCHABP12 OX=1965454 GN=ABP12_00055 PE=4 SV=1
MM1 pKa = 7.55 TKK3 pKa = 10.07 TSIDD7 pKa = 3.57 QEE9 pKa = 3.77 IDD11 pKa = 2.99 EE12 pKa = 5.74 AIAKK16 pKa = 9.91 GGSFYY21 pKa = 10.86 KK22 pKa = 10.0 IRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 FEE28 pKa = 3.54 KK29 pKa = 10.65 AILEE33 pKa = 4.06 RR34 pKa = 11.84 ALIKK38 pKa = 10.08 TRR40 pKa = 11.84 GNQTEE45 pKa = 4.03 AAKK48 pKa = 9.89 MLGISRR54 pKa = 11.84 SGLGGILKK62 pKa = 10.32 RR63 pKa = 11.84 VGRR66 pKa = 4.23
Molecular weight: 7.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.276
IPC2_protein 9.692
IPC_protein 10.292
Toseland 10.965
ProMoST 10.994
Dawson 11.008
Bjellqvist 10.687
Wikipedia 11.199
Rodwell 11.33
Grimsley 11.038
Solomon 11.169
Lehninger 11.14
Nozaki 10.935
DTASelect 10.687
Thurlkill 10.935
EMBOSS 11.359
Sillero 10.95
Patrickios 11.096
IPC_peptide 11.169
IPC2_peptide 9.238
IPC2.peptide.svr19 8.862
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
88
0
88
14170
37
732
161.0
18.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.867 ± 0.315
1.27 ± 0.125
6.168 ± 0.264
6.683 ± 0.358
4.404 ± 0.202
6.994 ± 0.322
1.637 ± 0.123
7.191 ± 0.323
7.692 ± 0.347
8.059 ± 0.274
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.879 ± 0.134
5.872 ± 0.267
2.929 ± 0.141
3.733 ± 0.235
3.754 ± 0.189
6.429 ± 0.205
5.434 ± 0.334
6.471 ± 0.262
1.482 ± 0.123
4.051 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here