Streptomyces agglomeratus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7342 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5NZ60|A0A1E5NZ60_9ACTN Transposase OS=Streptomyces agglomeratus OX=285458 GN=AS594_38555 PE=3 SV=1
MM1 pKa = 6.93NAEE4 pKa = 4.33VAWGGRR10 pKa = 11.84WEE12 pKa = 4.25HH13 pKa = 6.62PEE15 pKa = 4.09CGASGEE21 pKa = 4.5AVWDD25 pKa = 4.47DD26 pKa = 4.82DD27 pKa = 3.89DD28 pKa = 4.35TASSGHH34 pKa = 6.32DD35 pKa = 3.31CDD37 pKa = 3.67RR38 pKa = 11.84TGEE41 pKa = 4.35VTWNAEE47 pKa = 4.39WKK49 pKa = 10.36CHH51 pKa = 4.38GCGTGSDD58 pKa = 4.08DD59 pKa = 5.49QFDD62 pKa = 5.16DD63 pKa = 4.3DD64 pKa = 4.44TTTHH68 pKa = 7.16ADD70 pKa = 3.15HH71 pKa = 7.28EE72 pKa = 4.63YY73 pKa = 10.72ADD75 pKa = 4.03EE76 pKa = 5.05DD77 pKa = 3.9EE78 pKa = 5.34GVAAA82 pKa = 5.65

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5PGH0|A0A1E5PGH0_9ACTN Antitermination regulator OS=Streptomyces agglomeratus OX=285458 GN=AS594_33270 PE=4 SV=1
MM1 pKa = 8.12PITFRR6 pKa = 11.84KK7 pKa = 9.4SFRR10 pKa = 11.84ILPGVRR16 pKa = 11.84LNINRR21 pKa = 11.84RR22 pKa = 11.84SWSITTGGRR31 pKa = 11.84NGPRR35 pKa = 11.84RR36 pKa = 11.84TTSSTGRR43 pKa = 11.84RR44 pKa = 11.84TTSVDD49 pKa = 3.27LPGPFGWRR57 pKa = 11.84KK58 pKa = 4.1TTRR61 pKa = 11.84TRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 3.23

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7342

0

7342

2324026

29

3566

316.5

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.653 ± 0.045

0.825 ± 0.009

5.922 ± 0.024

5.743 ± 0.028

2.725 ± 0.016

9.429 ± 0.028

2.298 ± 0.013

3.147 ± 0.018

2.334 ± 0.028

10.195 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.799 ± 0.012

1.814 ± 0.016

6.058 ± 0.03

2.842 ± 0.018

8.051 ± 0.032

5.09 ± 0.021

6.092 ± 0.024

8.337 ± 0.029

1.535 ± 0.013

2.109 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski