Amino acid dipepetide frequency for Streptomyces agglomeratus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.826AlaAla: 21.826 ± 0.169
1.105AlaCys: 1.105 ± 0.025
8.183AlaAsp: 8.183 ± 0.061
8.783AlaGlu: 8.783 ± 0.102
3.468AlaPhe: 3.468 ± 0.043
13.498AlaGly: 13.498 ± 0.093
2.888AlaHis: 2.888 ± 0.043
3.408AlaIle: 3.408 ± 0.045
3.076AlaLys: 3.076 ± 0.052
14.286AlaLeu: 14.286 ± 0.106
2.665AlaMet: 2.665 ± 0.032
1.956AlaAsn: 1.956 ± 0.029
7.228AlaPro: 7.228 ± 0.083
3.882AlaGln: 3.882 ± 0.048
10.098AlaArg: 10.098 ± 0.093
6.017AlaSer: 6.017 ± 0.05
7.045AlaThr: 7.045 ± 0.066
12.334AlaVal: 12.334 ± 0.089
1.897AlaTrp: 1.897 ± 0.03
2.886AlaTyr: 2.886 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.145CysAla: 1.145 ± 0.021
0.099CysCys: 0.099 ± 0.007
0.491CysAsp: 0.491 ± 0.015
0.432CysGlu: 0.432 ± 0.013
0.242CysPhe: 0.242 ± 0.01
1.007CysGly: 1.007 ± 0.022
0.198CysHis: 0.198 ± 0.01
0.159CysIle: 0.159 ± 0.009
0.133CysLys: 0.133 ± 0.007
0.77CysLeu: 0.77 ± 0.02
0.13CysMet: 0.13 ± 0.007
0.143CysAsn: 0.143 ± 0.007
0.535CysPro: 0.535 ± 0.017
0.177CysGln: 0.177 ± 0.009
0.619CysArg: 0.619 ± 0.018
0.438CysSer: 0.438 ± 0.016
0.532CysThr: 0.532 ± 0.016
0.7CysVal: 0.7 ± 0.017
0.138CysTrp: 0.138 ± 0.007
0.157CysTyr: 0.157 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.786AspAla: 7.786 ± 0.076
0.454AspCys: 0.454 ± 0.014
3.589AspAsp: 3.589 ± 0.047
3.935AspGlu: 3.935 ± 0.049
1.678AspPhe: 1.678 ± 0.031
6.194AspGly: 6.194 ± 0.06
1.361AspHis: 1.361 ± 0.026
1.94AspIle: 1.94 ± 0.029
1.342AspLys: 1.342 ± 0.027
5.972AspLeu: 5.972 ± 0.057
0.844AspMet: 0.844 ± 0.021
1.017AspAsn: 1.017 ± 0.024
4.304AspPro: 4.304 ± 0.048
1.557AspGln: 1.557 ± 0.028
4.852AspArg: 4.852 ± 0.048
2.583AspSer: 2.583 ± 0.036
3.108AspThr: 3.108 ± 0.041
4.576AspVal: 4.576 ± 0.046
1.03AspTrp: 1.03 ± 0.022
1.098AspTyr: 1.098 ± 0.023
0.0AspXaa: 0.0 ± 0.0
Glu
7.47GluAla: 7.47 ± 0.084
0.394GluCys: 0.394 ± 0.013
2.784GluAsp: 2.784 ± 0.035
3.587GluGlu: 3.587 ± 0.05
1.431GluPhe: 1.431 ± 0.029
4.392GluGly: 4.392 ± 0.048
1.518GluHis: 1.518 ± 0.025
2.346GluIle: 2.346 ± 0.037
1.599GluLys: 1.599 ± 0.036
6.799GluLeu: 6.799 ± 0.067
0.932GluMet: 0.932 ± 0.018
1.096GluAsn: 1.096 ± 0.022
3.499GluPro: 3.499 ± 0.046
2.307GluGln: 2.307 ± 0.036
5.541GluArg: 5.541 ± 0.06
2.568GluSer: 2.568 ± 0.034
2.922GluThr: 2.922 ± 0.041
4.348GluVal: 4.348 ± 0.048
0.784GluTrp: 0.784 ± 0.019
1.117GluTyr: 1.117 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.691PheAla: 3.691 ± 0.048
0.296PheCys: 0.296 ± 0.011
1.938PheAsp: 1.938 ± 0.032
1.454PheGlu: 1.454 ± 0.025
0.91PhePhe: 0.91 ± 0.023
2.981PheGly: 2.981 ± 0.042
0.617PheHis: 0.617 ± 0.016
0.712PheIle: 0.712 ± 0.019
0.58PheLys: 0.58 ± 0.018
2.544PheLeu: 2.544 ± 0.037
0.415PheMet: 0.415 ± 0.014
0.563PheAsn: 0.563 ± 0.017
1.346PhePro: 1.346 ± 0.027
0.697PheGln: 0.697 ± 0.018
1.808PheArg: 1.808 ± 0.027
1.47PheSer: 1.47 ± 0.026
2.016PheThr: 2.016 ± 0.029
2.197PheVal: 2.197 ± 0.034
0.438PheTrp: 0.438 ± 0.016
0.576PheTyr: 0.576 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
11.211GlyAla: 11.211 ± 0.098
0.865GlyCys: 0.865 ± 0.02
5.206GlyAsp: 5.206 ± 0.055
5.189GlyGlu: 5.189 ± 0.048
2.871GlyPhe: 2.871 ± 0.043
8.963GlyGly: 8.963 ± 0.09
2.304GlyHis: 2.304 ± 0.033
3.443GlyIle: 3.443 ± 0.043
2.631GlyLys: 2.631 ± 0.041
9.26GlyLeu: 9.26 ± 0.075
2.035GlyMet: 2.035 ± 0.032
1.765GlyAsn: 1.765 ± 0.029
5.169GlyPro: 5.169 ± 0.048
2.749GlyGln: 2.749 ± 0.042
7.788GlyArg: 7.788 ± 0.068
5.415GlySer: 5.415 ± 0.056
6.196GlyThr: 6.196 ± 0.06
7.338GlyVal: 7.338 ± 0.064
1.674GlyTrp: 1.674 ± 0.031
2.222GlyTyr: 2.222 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
2.689HisAla: 2.689 ± 0.034
0.222HisCys: 0.222 ± 0.011
1.338HisAsp: 1.338 ± 0.023
1.217HisGlu: 1.217 ± 0.019
0.687HisPhe: 0.687 ± 0.019
2.35HisGly: 2.35 ± 0.031
0.704HisHis: 0.704 ± 0.018
0.689HisIle: 0.689 ± 0.019
0.389HisLys: 0.389 ± 0.013
2.466HisLeu: 2.466 ± 0.035
0.355HisMet: 0.355 ± 0.011
0.417HisAsn: 0.417 ± 0.015
1.785HisPro: 1.785 ± 0.036
0.699HisGln: 0.699 ± 0.017
2.092HisArg: 2.092 ± 0.033
1.072HisSer: 1.072 ± 0.024
1.34HisThr: 1.34 ± 0.024
1.614HisVal: 1.614 ± 0.027
0.372HisTrp: 0.372 ± 0.011
0.482HisTyr: 0.482 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.759IleAla: 4.759 ± 0.046
0.315IleCys: 0.315 ± 0.012
2.245IleAsp: 2.245 ± 0.034
1.998IleGlu: 1.998 ± 0.034
0.71IlePhe: 0.71 ± 0.02
3.52IleGly: 3.52 ± 0.04
0.614IleHis: 0.614 ± 0.017
0.863IleIle: 0.863 ± 0.026
0.784IleLys: 0.784 ± 0.021
2.346IleLeu: 2.346 ± 0.034
0.437IleMet: 0.437 ± 0.014
0.731IleAsn: 0.731 ± 0.019
1.715IlePro: 1.715 ± 0.03
0.725IleGln: 0.725 ± 0.017
2.243IleArg: 2.243 ± 0.033
1.703IleSer: 1.703 ± 0.032
2.255IleThr: 2.255 ± 0.032
2.631IleVal: 2.631 ± 0.041
0.369IleTrp: 0.369 ± 0.013
0.507IleTyr: 0.507 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
3.127LysAla: 3.127 ± 0.05
0.134LysCys: 0.134 ± 0.008
1.419LysAsp: 1.419 ± 0.029
1.271LysGlu: 1.271 ± 0.027
0.503LysPhe: 0.503 ± 0.018
1.958LysGly: 1.958 ± 0.039
0.476LysHis: 0.476 ± 0.016
0.954LysIle: 0.954 ± 0.023
0.983LysLys: 0.983 ± 0.028
2.191LysLeu: 2.191 ± 0.038
0.435LysMet: 0.435 ± 0.012
0.593LysAsn: 0.593 ± 0.018
1.452LysPro: 1.452 ± 0.032
0.747LysGln: 0.747 ± 0.019
1.611LysArg: 1.611 ± 0.029
1.252LysSer: 1.252 ± 0.026
1.423LysThr: 1.423 ± 0.033
1.983LysVal: 1.983 ± 0.036
0.311LysTrp: 0.311 ± 0.012
0.522LysTyr: 0.522 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
14.873LeuAla: 14.873 ± 0.099
0.878LeuCys: 0.878 ± 0.018
6.54LeuAsp: 6.54 ± 0.055
4.812LeuGlu: 4.812 ± 0.048
2.605LeuPhe: 2.605 ± 0.037
9.093LeuGly: 9.093 ± 0.07
2.28LeuHis: 2.28 ± 0.034
3.254LeuIle: 3.254 ± 0.042
2.141LeuLys: 2.141 ± 0.033
11.089LeuLeu: 11.089 ± 0.098
1.723LeuMet: 1.723 ± 0.032
1.722LeuAsn: 1.722 ± 0.031
6.397LeuPro: 6.397 ± 0.059
2.177LeuGln: 2.177 ± 0.034
8.61LeuArg: 8.61 ± 0.073
5.335LeuSer: 5.335 ± 0.05
6.688LeuThr: 6.688 ± 0.05
8.561LeuVal: 8.561 ± 0.067
1.332LeuTrp: 1.332 ± 0.023
1.842LeuTyr: 1.842 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.32MetAla: 2.32 ± 0.033
0.142MetCys: 0.142 ± 0.008
0.947MetAsp: 0.947 ± 0.021
0.827MetGlu: 0.827 ± 0.02
0.456MetPhe: 0.456 ± 0.014
1.326MetGly: 1.326 ± 0.026
0.371MetHis: 0.371 ± 0.013
0.681MetIle: 0.681 ± 0.019
0.467MetLys: 0.467 ± 0.014
1.744MetLeu: 1.744 ± 0.026
0.315MetMet: 0.315 ± 0.011
0.465MetAsn: 0.465 ± 0.013
1.197MetPro: 1.197 ± 0.021
0.467MetGln: 0.467 ± 0.013
1.521MetArg: 1.521 ± 0.028
1.328MetSer: 1.328 ± 0.023
1.544MetThr: 1.544 ± 0.024
1.299MetVal: 1.299 ± 0.022
0.223MetTrp: 0.223 ± 0.009
0.35MetTyr: 0.35 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.284AsnAla: 2.284 ± 0.034
0.165AsnCys: 0.165 ± 0.009
1.001AsnAsp: 1.001 ± 0.023
0.889AsnGlu: 0.889 ± 0.021
0.487AsnPhe: 0.487 ± 0.015
1.907AsnGly: 1.907 ± 0.033
0.414AsnHis: 0.414 ± 0.014
0.664AsnIle: 0.664 ± 0.019
0.461AsnLys: 0.461 ± 0.015
1.677AsnLeu: 1.677 ± 0.033
0.309AsnMet: 0.309 ± 0.011
0.453AsnAsn: 0.453 ± 0.016
1.363AsnPro: 1.363 ± 0.028
0.519AsnGln: 0.519 ± 0.016
1.359AsnArg: 1.359 ± 0.024
0.932AsnSer: 0.932 ± 0.023
1.167AsnThr: 1.167 ± 0.023
1.395AsnVal: 1.395 ± 0.026
0.289AsnTrp: 0.289 ± 0.012
0.404AsnTyr: 0.404 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.879ProAla: 8.879 ± 0.084
0.342ProCys: 0.342 ± 0.012
4.349ProAsp: 4.349 ± 0.046
4.194ProGlu: 4.194 ± 0.047
1.523ProPhe: 1.523 ± 0.024
6.652ProGly: 6.652 ± 0.071
1.418ProHis: 1.418 ± 0.024
1.169ProIle: 1.169 ± 0.026
1.268ProLys: 1.268 ± 0.03
5.271ProLeu: 5.271 ± 0.051
1.022ProMet: 1.022 ± 0.022
0.91ProAsn: 0.91 ± 0.021
3.604ProPro: 3.604 ± 0.055
1.915ProGln: 1.915 ± 0.04
3.9ProArg: 3.9 ± 0.044
3.194ProSer: 3.194 ± 0.047
3.015ProThr: 3.015 ± 0.042
5.599ProVal: 5.599 ± 0.043
0.905ProTrp: 0.905 ± 0.023
1.454ProTyr: 1.454 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.799GlnAla: 3.799 ± 0.049
0.197GlnCys: 0.197 ± 0.008
1.449GlnAsp: 1.449 ± 0.025
1.539GlnGlu: 1.539 ± 0.026
0.707GlnPhe: 0.707 ± 0.017
2.275GlnGly: 2.275 ± 0.032
0.722GlnHis: 0.722 ± 0.018
1.089GlnIle: 1.089 ± 0.023
0.642GlnLys: 0.642 ± 0.017
3.099GlnLeu: 3.099 ± 0.042
0.537GlnMet: 0.537 ± 0.017
0.508GlnAsn: 0.508 ± 0.017
1.813GlnPro: 1.813 ± 0.038
1.388GlnGln: 1.388 ± 0.049
2.454GlnArg: 2.454 ± 0.037
1.327GlnSer: 1.327 ± 0.025
1.345GlnThr: 1.345 ± 0.023
2.387GlnVal: 2.387 ± 0.03
0.469GlnTrp: 0.469 ± 0.016
0.674GlnTyr: 0.674 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
9.891ArgAla: 9.891 ± 0.075
0.63ArgCys: 0.63 ± 0.017
4.317ArgAsp: 4.317 ± 0.048
4.847ArgGlu: 4.847 ± 0.053
2.291ArgPhe: 2.291 ± 0.034
5.855ArgGly: 5.855 ± 0.056
2.088ArgHis: 2.088 ± 0.03
3.183ArgIle: 3.183 ± 0.035
1.787ArgLys: 1.787 ± 0.031
8.722ArgLeu: 8.722 ± 0.072
1.747ArgMet: 1.747 ± 0.029
1.441ArgAsn: 1.441 ± 0.026
4.958ArgPro: 4.958 ± 0.05
2.364ArgGln: 2.364 ± 0.038
7.798ArgArg: 7.798 ± 0.08
4.086ArgSer: 4.086 ± 0.046
5.629ArgThr: 5.629 ± 0.052
5.689ArgVal: 5.689 ± 0.053
1.412ArgTrp: 1.412 ± 0.026
1.778ArgTyr: 1.778 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.773SerAla: 6.773 ± 0.054
0.437SerCys: 0.437 ± 0.013
2.747SerAsp: 2.747 ± 0.034
2.484SerGlu: 2.484 ± 0.042
1.535SerPhe: 1.535 ± 0.028
5.992SerGly: 5.992 ± 0.055
1.05SerHis: 1.05 ± 0.022
1.378SerIle: 1.378 ± 0.026
1.113SerLys: 1.113 ± 0.024
4.826SerLeu: 4.826 ± 0.04
1.074SerMet: 1.074 ± 0.022
0.889SerAsn: 0.889 ± 0.021
3.184SerPro: 3.184 ± 0.04
1.312SerGln: 1.312 ± 0.023
3.807SerArg: 3.807 ± 0.039
2.823SerSer: 2.823 ± 0.043
3.039SerThr: 3.039 ± 0.038
4.311SerVal: 4.311 ± 0.046
0.908SerTrp: 0.908 ± 0.021
1.219SerTyr: 1.219 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
8.922ThrAla: 8.922 ± 0.069
0.446ThrCys: 0.446 ± 0.016
3.657ThrAsp: 3.657 ± 0.044
3.227ThrGlu: 3.227 ± 0.036
1.605ThrPhe: 1.605 ± 0.029
6.707ThrGly: 6.707 ± 0.054
1.245ThrHis: 1.245 ± 0.022
1.63ThrIle: 1.63 ± 0.026
1.231ThrLys: 1.231 ± 0.026
5.667ThrLeu: 5.667 ± 0.052
0.964ThrMet: 0.964 ± 0.025
1.003ThrAsn: 1.003 ± 0.022
3.949ThrPro: 3.949 ± 0.051
1.385ThrGln: 1.385 ± 0.024
3.972ThrArg: 3.972 ± 0.035
3.157ThrSer: 3.157 ± 0.041
3.845ThrThr: 3.845 ± 0.055
5.952ThrVal: 5.952 ± 0.043
0.926ThrTrp: 0.926 ± 0.022
1.43ThrTyr: 1.43 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.48ValAla: 10.48 ± 0.078
0.768ValCys: 0.768 ± 0.019
4.725ValAsp: 4.725 ± 0.046
4.679ValGlu: 4.679 ± 0.052
2.366ValPhe: 2.366 ± 0.036
6.312ValGly: 6.312 ± 0.052
1.961ValHis: 1.961 ± 0.03
2.827ValIle: 2.827 ± 0.039
1.847ValLys: 1.847 ± 0.032
9.37ValLeu: 9.37 ± 0.076
1.491ValMet: 1.491 ± 0.027
1.645ValAsn: 1.645 ± 0.029
5.267ValPro: 5.267 ± 0.046
2.113ValGln: 2.113 ± 0.034
7.216ValArg: 7.216 ± 0.06
4.288ValSer: 4.288 ± 0.042
5.527ValThr: 5.527 ± 0.059
7.774ValVal: 7.774 ± 0.071
1.165ValTrp: 1.165 ± 0.024
1.552ValTyr: 1.552 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.697TrpAla: 1.697 ± 0.029
0.172TrpCys: 0.172 ± 0.008
0.813TrpAsp: 0.813 ± 0.022
0.761TrpGlu: 0.761 ± 0.018
0.53TrpPhe: 0.53 ± 0.015
1.031TrpGly: 1.031 ± 0.024
0.386TrpHis: 0.386 ± 0.015
0.556TrpIle: 0.556 ± 0.017
0.418TrpLys: 0.418 ± 0.013
1.765TrpLeu: 1.765 ± 0.028
0.306TrpMet: 0.306 ± 0.012
0.417TrpAsn: 0.417 ± 0.012
0.778TrpPro: 0.778 ± 0.019
0.661TrpGln: 0.661 ± 0.018
1.356TrpArg: 1.356 ± 0.024
0.923TrpSer: 0.923 ± 0.022
1.064TrpThr: 1.064 ± 0.021
0.992TrpVal: 0.992 ± 0.021
0.359TrpTrp: 0.359 ± 0.012
0.37TrpTyr: 0.37 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.909TyrAla: 2.909 ± 0.035
0.185TyrCys: 0.185 ± 0.008
1.484TyrAsp: 1.484 ± 0.026
1.306TyrGlu: 1.306 ± 0.026
0.644TyrPhe: 0.644 ± 0.019
2.281TyrGly: 2.281 ± 0.034
0.367TyrHis: 0.367 ± 0.013
0.524TyrIle: 0.524 ± 0.016
0.454TyrLys: 0.454 ± 0.015
2.089TyrLeu: 2.089 ± 0.03
0.254TyrMet: 0.254 ± 0.01
0.409TyrAsn: 0.409 ± 0.013
1.04TyrPro: 1.04 ± 0.022
0.579TyrGln: 0.579 ± 0.015
1.868TyrArg: 1.868 ± 0.031
0.987TyrSer: 0.987 ± 0.021
1.219TyrThr: 1.219 ± 0.028
1.691TyrVal: 1.691 ± 0.027
0.35TyrTrp: 0.35 ± 0.012
0.453TyrTyr: 0.453 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7342 proteins (2324026 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski