Maricaulis salignorans
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2949 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9LDN2|A0A1G9LDN2_9PROT Uncharacterized protein OS=Maricaulis salignorans OX=144026 GN=SAMN04488568_1014 PE=4 SV=1
MM1 pKa = 7.89 ASTLISGLAVAAPAMAQDD19 pKa = 3.61 QDD21 pKa = 4.06 VVTVTGSRR29 pKa = 11.84 IQRR32 pKa = 11.84 TDD34 pKa = 3.36 TIAPSPVTNVSAEE47 pKa = 3.82 QLAVVNTVNTEE58 pKa = 3.94 DD59 pKa = 5.14 FINTLPQVVPAFDD72 pKa = 3.39 ATSNNPGDD80 pKa = 3.87 GTATVALRR88 pKa = 11.84 GLGATRR94 pKa = 11.84 TLVLVDD100 pKa = 3.33 GQRR103 pKa = 11.84 FVGSGVSQVVDD114 pKa = 4.28 LNNIPAAMVEE124 pKa = 4.45 SIDD127 pKa = 3.68 IVTGGASAVYY137 pKa = 10.33 GSDD140 pKa = 2.82 AVAGVVNFMMKK151 pKa = 10.48 DD152 pKa = 3.18 DD153 pKa = 4.13 FEE155 pKa = 4.8 GVEE158 pKa = 3.97 ITASQEE164 pKa = 3.83 TSLGDD169 pKa = 3.72 LDD171 pKa = 5.79 ADD173 pKa = 3.59 ITNVSITMGGNFDD186 pKa = 3.78 NGRR189 pKa = 11.84 GNAVLSMGYY198 pKa = 7.5 TNRR201 pKa = 11.84 QALFQGDD208 pKa = 3.8 RR209 pKa = 11.84 AFSALTNWDD218 pKa = 3.84 PGPGNEE224 pKa = 4.87 AAGFYY229 pKa = 10.54 AGGSSNIPGTRR240 pKa = 11.84 IRR242 pKa = 11.84 GGGSTNFGLMNYY254 pKa = 8.7 DD255 pKa = 3.41 ANGNEE260 pKa = 4.08 IDD262 pKa = 4.39 SSNTSYY268 pKa = 10.8 LASDD272 pKa = 4.74 RR273 pKa = 11.84 IYY275 pKa = 10.71 DD276 pKa = 3.94 IFPEE280 pKa = 4.71 CGAGNALACGGVFNDD295 pKa = 3.77 GGIRR299 pKa = 11.84 GMRR302 pKa = 11.84 FGGATPDD309 pKa = 3.42 VYY311 pKa = 11.41 NYY313 pKa = 11.18 APANYY318 pKa = 9.33 LQLPQEE324 pKa = 4.68 RR325 pKa = 11.84 YY326 pKa = 9.96 NISAFATYY334 pKa = 9.5 EE335 pKa = 4.19 VNEE338 pKa = 3.96 HH339 pKa = 6.34 LEE341 pKa = 4.21 FYY343 pKa = 11.16 GRR345 pKa = 11.84 GIYY348 pKa = 10.3 ANNVVDD354 pKa = 4.2 SQLAPTPAGVTLNVNLDD371 pKa = 3.9 NPNLPADD378 pKa = 4.09 LVDD381 pKa = 4.86 LLVGDD386 pKa = 4.14 VDD388 pKa = 4.23 AVVATGLADD397 pKa = 4.57 DD398 pKa = 5.52 PGTAADD404 pKa = 3.54 EE405 pKa = 4.33 SVYY408 pKa = 11.1 NHH410 pKa = 6.46 SGSNNGDD417 pKa = 2.89 GTATVRR423 pKa = 11.84 ISRR426 pKa = 11.84 RR427 pKa = 11.84 FEE429 pKa = 4.25 EE430 pKa = 3.87 IGTRR434 pKa = 11.84 NSLRR438 pKa = 11.84 DD439 pKa = 3.55 TNSFQVVFGARR450 pKa = 11.84 GALNEE455 pKa = 4.02 NWSYY459 pKa = 11.86 DD460 pKa = 2.89 AFANYY465 pKa = 9.45 SRR467 pKa = 11.84 SAVSQIQTGNLSVSALQDD485 pKa = 3.31 GVLCDD490 pKa = 4.28 GGPTALASGCSAPYY504 pKa = 10.26 VDD506 pKa = 5.52 LFNGPGAISAAGASHH521 pKa = 7.62 ISRR524 pKa = 11.84 TGAQIDD530 pKa = 4.32 TIEE533 pKa = 4.14 MFQTQATVNGVLPMFQSPAANEE555 pKa = 3.78 PLALVLGVEE564 pKa = 4.23 YY565 pKa = 10.8 RR566 pKa = 11.84 EE567 pKa = 4.36 ASSNSLPDD575 pKa = 3.51 SVLGPDD581 pKa = 3.33 VRR583 pKa = 11.84 GFNQALPVGGTVDD596 pKa = 3.12 VYY598 pKa = 11.48 EE599 pKa = 4.66 FFTEE603 pKa = 4.26 VEE605 pKa = 4.15 MPLIEE610 pKa = 5.12 DD611 pKa = 3.55 RR612 pKa = 11.84 PGIQSLSLNAAFRR625 pKa = 11.84 SSEE628 pKa = 4.01 YY629 pKa = 9.21 NTVGGTEE636 pKa = 4.04 TFAVGLGWEE645 pKa = 4.38 VNDD648 pKa = 4.59 QIRR651 pKa = 11.84 LRR653 pKa = 11.84 GNFNRR658 pKa = 11.84 AVRR661 pKa = 11.84 APNVGDD667 pKa = 4.35 LYY669 pKa = 11.18 QPLVNGFPSAKK680 pKa = 9.58 DD681 pKa = 3.38 PCSGGSNGSYY691 pKa = 10.39 GAATIDD697 pKa = 3.65 QTCVDD702 pKa = 4.65 AGVPAPGAAIQGNAQMEE719 pKa = 4.44 ALFGGNPNLDD729 pKa = 3.59 AEE731 pKa = 4.6 TADD734 pKa = 3.65 TYY736 pKa = 11.12 TIGVVLTPDD745 pKa = 4.16 AIEE748 pKa = 4.47 GLTMTLDD755 pKa = 3.69 YY756 pKa = 11.24 YY757 pKa = 11.28 NISIEE762 pKa = 4.22 DD763 pKa = 4.56 AISTVPLQTLLNEE776 pKa = 4.09 CHH778 pKa = 6.93 IDD780 pKa = 4.31 NIAASCTALAGGRR793 pKa = 11.84 GPSGEE798 pKa = 3.93 MGANGFLPSLVSINVASLEE817 pKa = 4.05 AEE819 pKa = 4.78 GIDD822 pKa = 4.31 LRR824 pKa = 11.84 VDD826 pKa = 3.29 YY827 pKa = 11.31 GFGADD832 pKa = 3.7 VLGLPGDD839 pKa = 4.02 FAVTYY844 pKa = 9.98 YY845 pKa = 10.94 GGYY848 pKa = 8.82 TIANNFTPSASSPVVEE864 pKa = 4.36 CAGFFGLDD872 pKa = 3.54 CGEE875 pKa = 4.04 PTPEE879 pKa = 4.54 YY880 pKa = 9.19 KK881 pKa = 10.4 HH882 pKa = 6.85 SMQASWYY889 pKa = 7.53 TGPFTTSLRR898 pKa = 11.84 WRR900 pKa = 11.84 YY901 pKa = 9.61 IGAVEE906 pKa = 4.57 ADD908 pKa = 3.83 SATAPQVSSLSDD920 pKa = 3.69 SIDD923 pKa = 3.01 ATNYY927 pKa = 10.05 FDD929 pKa = 4.64 VTFQYY934 pKa = 10.8 DD935 pKa = 3.28 INDD938 pKa = 3.96 TFTLTTGISNITDD951 pKa = 3.6 TEE953 pKa = 4.55 VPAIGSTSTEE963 pKa = 4.13 QANTWPATYY972 pKa = 7.55 EE973 pKa = 3.96 TLGRR977 pKa = 11.84 RR978 pKa = 11.84 LFVGGTMRR986 pKa = 11.84 FF987 pKa = 3.5
Molecular weight: 103.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.897
IPC_protein 3.923
Toseland 3.706
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.834
Rodwell 3.745
Grimsley 3.605
Solomon 3.91
Lehninger 3.859
Nozaki 4.012
DTASelect 4.253
Thurlkill 3.745
EMBOSS 3.834
Sillero 4.037
Patrickios 1.176
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.933
Protein with the highest isoelectric point:
>tr|A0A1G9SQW3|A0A1G9SQW3_9PROT Lipopolysaccharide export system ATP-binding protein LptB OS=Maricaulis salignorans OX=144026 GN=SAMN04488568_11042 PE=3 SV=1
MM1 pKa = 6.89 TQTGRR6 pKa = 11.84 IRR8 pKa = 11.84 LGDD11 pKa = 3.39 KK12 pKa = 10.29 DD13 pKa = 3.09 IPYY16 pKa = 10.86 ALRR19 pKa = 11.84 RR20 pKa = 11.84 STRR23 pKa = 11.84 RR24 pKa = 11.84 TIGFVINRR32 pKa = 11.84 DD33 pKa = 3.75 GLSVTAPQRR42 pKa = 11.84 VSVRR46 pKa = 11.84 DD47 pKa = 3.37 IEE49 pKa = 4.7 AALQEE54 pKa = 4.19 KK55 pKa = 8.72 SHH57 pKa = 6.7 WILGKK62 pKa = 9.89 LAAWAEE68 pKa = 4.21 RR69 pKa = 11.84 PHH71 pKa = 6.51 QRR73 pKa = 11.84 EE74 pKa = 3.72 LAYY77 pKa = 9.82 VTGEE81 pKa = 4.0 TLPWLGGEE89 pKa = 4.68 LKK91 pKa = 10.83 LQVEE95 pKa = 4.52 PRR97 pKa = 11.84 GIRR100 pKa = 11.84 TTLRR104 pKa = 11.84 RR105 pKa = 11.84 DD106 pKa = 3.09 GDD108 pKa = 3.9 RR109 pKa = 11.84 VLVTVDD115 pKa = 3.88 PEE117 pKa = 4.01 ASGEE121 pKa = 3.94 LRR123 pKa = 11.84 RR124 pKa = 11.84 RR125 pKa = 11.84 AIASALMRR133 pKa = 11.84 HH134 pKa = 5.67 YY135 pKa = 10.47 KK136 pKa = 10.27 RR137 pKa = 11.84 EE138 pKa = 4.17 GEE140 pKa = 4.02 HH141 pKa = 5.98 FMRR144 pKa = 11.84 PRR146 pKa = 11.84 VEE148 pKa = 4.72 AFATRR153 pKa = 11.84 LEE155 pKa = 4.16 RR156 pKa = 11.84 PVRR159 pKa = 11.84 KK160 pKa = 9.7 VVIRR164 pKa = 11.84 DD165 pKa = 3.28 QKK167 pKa = 10.82 RR168 pKa = 11.84 RR169 pKa = 11.84 WGSCAPDD176 pKa = 3.4 GTIRR180 pKa = 11.84 LNWRR184 pKa = 11.84 LMGFPEE190 pKa = 3.94 NLVDD194 pKa = 3.84 YY195 pKa = 10.59 VCAHH199 pKa = 6.72 EE200 pKa = 4.47 AAHH203 pKa = 6.27 LVEE206 pKa = 5.54 HH207 pKa = 6.0 NHH209 pKa = 6.19 SPAFWKK215 pKa = 9.05 TVEE218 pKa = 4.95 RR219 pKa = 11.84 IMPDD223 pKa = 2.65 WRR225 pKa = 11.84 IHH227 pKa = 5.69 RR228 pKa = 11.84 DD229 pKa = 3.31 EE230 pKa = 4.19 MRR232 pKa = 11.84 RR233 pKa = 11.84 AADD236 pKa = 3.05 LWTWVV241 pKa = 3.38
Molecular weight: 28.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.443
IPC_protein 10.409
Toseland 10.643
ProMoST 10.438
Dawson 10.716
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.76
Grimsley 10.774
Solomon 10.906
Lehninger 10.862
Nozaki 10.628
DTASelect 10.467
Thurlkill 10.643
EMBOSS 11.052
Sillero 10.657
Patrickios 10.467
IPC_peptide 10.906
IPC2_peptide 9.545
IPC2.peptide.svr19 8.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2949
0
2949
939932
29
2981
318.7
34.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.041 ± 0.063
0.825 ± 0.013
6.226 ± 0.041
5.948 ± 0.041
3.682 ± 0.026
8.726 ± 0.049
1.974 ± 0.02
5.243 ± 0.031
2.49 ± 0.043
10.058 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.522 ± 0.022
2.557 ± 0.024
5.022 ± 0.033
3.174 ± 0.021
7.173 ± 0.053
5.565 ± 0.037
5.248 ± 0.037
6.906 ± 0.037
1.408 ± 0.02
2.213 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here