Staphylococcus phage 23MRA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; Staphylococcus virus 23MRA

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3U2U6|A0A0H3U2U6_9CAUD Uncharacterized protein OS=Staphylococcus phage 23MRA OX=1505027 PE=4 SV=1
MM1 pKa = 8.04AIQLIQEE8 pKa = 4.16LQVYY12 pKa = 8.67YY13 pKa = 10.28PITQLSNMTAHH24 pKa = 7.36IALMAIDD31 pKa = 4.85GLLILLIVDD40 pKa = 3.45NVVII44 pKa = 4.48

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3U319|A0A0H3U319_9CAUD Anti-repressor OS=Staphylococcus phage 23MRA OX=1505027 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.45NKK4 pKa = 10.06KK5 pKa = 9.71LLLASTSNSDD15 pKa = 3.43KK16 pKa = 11.0RR17 pKa = 11.84LTKK20 pKa = 10.51LIHH23 pKa = 5.8FQYY26 pKa = 9.13KK27 pKa = 8.44TKK29 pKa = 10.65QGGSQPP35 pKa = 3.58

Molecular weight: 3.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11868

35

1225

179.8

20.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.839 ± 0.357

0.43 ± 0.08

6.025 ± 0.342

7.609 ± 0.385

4.129 ± 0.318

5.654 ± 0.477

1.753 ± 0.194

7.701 ± 0.297

9.698 ± 0.369

8.434 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.671 ± 0.275

6.673 ± 0.248

2.629 ± 0.214

3.783 ± 0.257

4.348 ± 0.334

5.923 ± 0.283

5.671 ± 0.22

5.662 ± 0.238

1.129 ± 0.143

4.238 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski