Amino acid dipepetide frequency for Staphylococcus phage 23MRA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.022AlaAla: 2.022 ± 0.644
0.253AlaCys: 0.253 ± 0.164
2.781AlaAsp: 2.781 ± 0.465
3.623AlaGlu: 3.623 ± 0.736
2.949AlaPhe: 2.949 ± 0.699
3.792AlaGly: 3.792 ± 0.576
0.758AlaHis: 0.758 ± 0.267
5.225AlaIle: 5.225 ± 0.731
5.477AlaLys: 5.477 ± 0.612
5.225AlaLeu: 5.225 ± 0.716
1.77AlaMet: 1.77 ± 0.409
3.539AlaAsn: 3.539 ± 0.576
1.938AlaPro: 1.938 ± 0.426
2.949AlaGln: 2.949 ± 0.511
2.781AlaArg: 2.781 ± 0.477
3.792AlaSer: 3.792 ± 0.626
3.034AlaThr: 3.034 ± 0.677
3.202AlaVal: 3.202 ± 0.6
0.59AlaTrp: 0.59 ± 0.2
2.697AlaTyr: 2.697 ± 0.376
0.0AlaXaa: 0.0 ± 0.0
Cys
0.253CysAla: 0.253 ± 0.171
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.169CysGlu: 0.169 ± 0.115
0.084CysPhe: 0.084 ± 0.087
0.169CysGly: 0.169 ± 0.134
0.084CysHis: 0.084 ± 0.098
0.59CysIle: 0.59 ± 0.229
0.253CysLys: 0.253 ± 0.173
0.169CysLeu: 0.169 ± 0.128
0.084CysMet: 0.084 ± 0.086
0.253CysAsn: 0.253 ± 0.143
0.169CysPro: 0.169 ± 0.119
0.169CysGln: 0.169 ± 0.129
0.421CysArg: 0.421 ± 0.217
0.253CysSer: 0.253 ± 0.135
0.421CysThr: 0.421 ± 0.17
0.253CysVal: 0.253 ± 0.144
0.169CysTrp: 0.169 ± 0.106
0.337CysTyr: 0.337 ± 0.182
0.0CysXaa: 0.0 ± 0.0
Asp
3.455AspAla: 3.455 ± 0.486
0.169AspCys: 0.169 ± 0.115
3.623AspAsp: 3.623 ± 0.711
5.225AspGlu: 5.225 ± 0.845
2.612AspPhe: 2.612 ± 0.542
5.393AspGly: 5.393 ± 0.733
0.843AspHis: 0.843 ± 0.279
4.803AspIle: 4.803 ± 0.621
5.477AspLys: 5.477 ± 0.897
5.562AspLeu: 5.562 ± 0.662
1.517AspMet: 1.517 ± 0.341
4.045AspAsn: 4.045 ± 0.612
1.348AspPro: 1.348 ± 0.456
0.59AspGln: 0.59 ± 0.21
2.191AspArg: 2.191 ± 0.53
3.286AspSer: 3.286 ± 0.647
2.949AspThr: 2.949 ± 0.59
3.708AspVal: 3.708 ± 0.53
0.758AspTrp: 0.758 ± 0.263
2.697AspTyr: 2.697 ± 0.587
0.0AspXaa: 0.0 ± 0.0
Glu
4.466GluAla: 4.466 ± 0.773
0.253GluCys: 0.253 ± 0.149
3.792GluAsp: 3.792 ± 0.499
5.899GluGlu: 5.899 ± 0.775
2.612GluPhe: 2.612 ± 0.509
2.528GluGly: 2.528 ± 0.483
1.348GluHis: 1.348 ± 0.344
6.152GluIle: 6.152 ± 0.796
7.584GluLys: 7.584 ± 1.013
7.416GluLeu: 7.416 ± 1.011
1.938GluMet: 1.938 ± 0.384
5.225GluAsn: 5.225 ± 0.568
1.601GluPro: 1.601 ± 0.307
3.202GluGln: 3.202 ± 0.537
4.213GluArg: 4.213 ± 0.619
4.719GluSer: 4.719 ± 0.712
3.792GluThr: 3.792 ± 0.916
4.466GluVal: 4.466 ± 0.49
0.927GluTrp: 0.927 ± 0.285
3.961GluTyr: 3.961 ± 0.757
0.0GluXaa: 0.0 ± 0.0
Phe
2.612PheAla: 2.612 ± 0.551
0.084PheCys: 0.084 ± 0.083
3.118PheAsp: 3.118 ± 0.759
3.455PheGlu: 3.455 ± 0.525
1.18PhePhe: 1.18 ± 0.277
2.191PheGly: 2.191 ± 0.392
0.506PheHis: 0.506 ± 0.171
2.949PheIle: 2.949 ± 0.597
4.213PheLys: 4.213 ± 0.662
2.865PheLeu: 2.865 ± 0.56
1.854PheMet: 1.854 ± 0.501
4.213PheAsn: 4.213 ± 0.49
0.843PhePro: 0.843 ± 0.326
0.674PheGln: 0.674 ± 0.213
1.348PheArg: 1.348 ± 0.291
3.034PheSer: 3.034 ± 0.675
2.781PheThr: 2.781 ± 0.424
2.022PheVal: 2.022 ± 0.419
0.169PheTrp: 0.169 ± 0.113
1.18PheTyr: 1.18 ± 0.299
0.0PheXaa: 0.0 ± 0.0
Gly
2.697GlyAla: 2.697 ± 0.538
0.421GlyCys: 0.421 ± 0.194
3.539GlyAsp: 3.539 ± 0.658
3.286GlyGlu: 3.286 ± 0.818
2.949GlyPhe: 2.949 ± 0.568
3.708GlyGly: 3.708 ± 1.031
1.011GlyHis: 1.011 ± 0.226
4.298GlyIle: 4.298 ± 0.834
5.899GlyLys: 5.899 ± 0.634
5.646GlyLeu: 5.646 ± 0.815
1.011GlyMet: 1.011 ± 0.289
3.118GlyAsn: 3.118 ± 0.513
1.601GlyPro: 1.601 ± 0.335
1.433GlyGln: 1.433 ± 0.381
2.949GlyArg: 2.949 ± 0.608
2.781GlySer: 2.781 ± 0.496
3.202GlyThr: 3.202 ± 0.553
3.539GlyVal: 3.539 ± 0.612
0.927GlyTrp: 0.927 ± 0.305
2.528GlyTyr: 2.528 ± 0.574
0.0GlyXaa: 0.0 ± 0.0
His
1.011HisAla: 1.011 ± 0.322
0.169HisCys: 0.169 ± 0.113
1.095HisAsp: 1.095 ± 0.359
0.758HisGlu: 0.758 ± 0.253
1.433HisPhe: 1.433 ± 0.389
0.674HisGly: 0.674 ± 0.259
0.337HisHis: 0.337 ± 0.19
2.107HisIle: 2.107 ± 0.414
1.348HisLys: 1.348 ± 0.331
1.433HisLeu: 1.433 ± 0.353
0.337HisMet: 0.337 ± 0.213
1.011HisAsn: 1.011 ± 0.301
0.421HisPro: 0.421 ± 0.189
0.927HisGln: 0.927 ± 0.284
0.421HisArg: 0.421 ± 0.173
0.843HisSer: 0.843 ± 0.228
0.843HisThr: 0.843 ± 0.29
0.674HisVal: 0.674 ± 0.263
0.169HisTrp: 0.169 ± 0.139
1.517HisTyr: 1.517 ± 0.367
0.0HisXaa: 0.0 ± 0.0
Ile
5.646IleAla: 5.646 ± 0.747
0.169IleCys: 0.169 ± 0.101
5.309IleAsp: 5.309 ± 0.542
6.657IleGlu: 6.657 ± 0.787
2.949IlePhe: 2.949 ± 0.506
4.129IleGly: 4.129 ± 0.535
1.77IleHis: 1.77 ± 0.428
4.382IleIle: 4.382 ± 0.585
8.09IleLys: 8.09 ± 0.633
4.635IleLeu: 4.635 ± 0.595
1.77IleMet: 1.77 ± 0.342
5.562IleAsn: 5.562 ± 0.898
1.854IlePro: 1.854 ± 0.55
3.623IleGln: 3.623 ± 0.639
2.781IleArg: 2.781 ± 0.474
4.635IleSer: 4.635 ± 0.671
4.213IleThr: 4.213 ± 0.528
4.719IleVal: 4.719 ± 0.566
1.011IleTrp: 1.011 ± 0.442
3.118IleTyr: 3.118 ± 0.679
0.0IleXaa: 0.0 ± 0.0
Lys
5.225LysAla: 5.225 ± 0.54
0.253LysCys: 0.253 ± 0.158
6.067LysAsp: 6.067 ± 0.737
8.09LysGlu: 8.09 ± 0.909
3.455LysPhe: 3.455 ± 0.517
6.152LysGly: 6.152 ± 0.933
1.433LysHis: 1.433 ± 0.427
6.573LysIle: 6.573 ± 0.839
8.258LysLys: 8.258 ± 0.872
7.837LysLeu: 7.837 ± 0.739
3.118LysMet: 3.118 ± 0.549
5.814LysAsn: 5.814 ± 0.931
2.359LysPro: 2.359 ± 0.556
4.719LysGln: 4.719 ± 0.691
4.803LysArg: 4.803 ± 0.741
5.14LysSer: 5.14 ± 0.646
6.826LysThr: 6.826 ± 0.803
5.983LysVal: 5.983 ± 0.671
1.011LysTrp: 1.011 ± 0.321
3.876LysTyr: 3.876 ± 0.694
0.0LysXaa: 0.0 ± 0.0
Leu
4.382LeuAla: 4.382 ± 0.808
0.674LeuCys: 0.674 ± 0.251
5.309LeuAsp: 5.309 ± 0.524
7.331LeuGlu: 7.331 ± 0.765
4.129LeuPhe: 4.129 ± 0.534
3.286LeuGly: 3.286 ± 0.649
1.433LeuHis: 1.433 ± 0.372
6.657LeuIle: 6.657 ± 0.905
9.438LeuLys: 9.438 ± 1.045
7.078LeuLeu: 7.078 ± 0.758
2.528LeuMet: 2.528 ± 0.541
5.562LeuAsn: 5.562 ± 0.847
2.359LeuPro: 2.359 ± 0.447
3.118LeuGln: 3.118 ± 0.471
3.708LeuArg: 3.708 ± 0.579
5.983LeuSer: 5.983 ± 0.687
4.298LeuThr: 4.298 ± 0.648
3.961LeuVal: 3.961 ± 0.543
0.59LeuTrp: 0.59 ± 0.224
2.444LeuTyr: 2.444 ± 0.487
0.0LeuXaa: 0.0 ± 0.0
Met
1.264MetAla: 1.264 ± 0.356
0.084MetCys: 0.084 ± 0.092
1.433MetAsp: 1.433 ± 0.397
1.601MetGlu: 1.601 ± 0.374
0.758MetPhe: 0.758 ± 0.215
1.18MetGly: 1.18 ± 0.436
0.253MetHis: 0.253 ± 0.161
2.022MetIle: 2.022 ± 0.44
2.444MetLys: 2.444 ± 0.49
2.697MetLeu: 2.697 ± 0.47
0.843MetMet: 0.843 ± 0.266
1.854MetAsn: 1.854 ± 0.409
1.095MetPro: 1.095 ± 0.279
1.011MetGln: 1.011 ± 0.317
1.517MetArg: 1.517 ± 0.312
1.77MetSer: 1.77 ± 0.372
2.107MetThr: 2.107 ± 0.46
1.601MetVal: 1.601 ± 0.308
0.506MetTrp: 0.506 ± 0.209
0.674MetTyr: 0.674 ± 0.213
0.0MetXaa: 0.0 ± 0.0
Asn
5.056AsnAla: 5.056 ± 0.672
0.169AsnCys: 0.169 ± 0.124
4.045AsnAsp: 4.045 ± 0.552
5.056AsnGlu: 5.056 ± 0.728
2.022AsnPhe: 2.022 ± 0.548
4.972AsnGly: 4.972 ± 0.546
0.927AsnHis: 0.927 ± 0.304
4.298AsnIle: 4.298 ± 0.606
6.152AsnLys: 6.152 ± 0.659
4.635AsnLeu: 4.635 ± 0.562
1.854AsnMet: 1.854 ± 0.376
4.466AsnAsn: 4.466 ± 0.616
2.612AsnPro: 2.612 ± 0.354
2.697AsnGln: 2.697 ± 0.443
2.612AsnArg: 2.612 ± 0.555
3.539AsnSer: 3.539 ± 0.423
3.792AsnThr: 3.792 ± 0.548
3.792AsnVal: 3.792 ± 0.637
1.011AsnTrp: 1.011 ± 0.363
3.034AsnTyr: 3.034 ± 0.554
0.0AsnXaa: 0.0 ± 0.0
Pro
1.095ProAla: 1.095 ± 0.331
0.084ProCys: 0.084 ± 0.08
1.18ProAsp: 1.18 ± 0.212
2.612ProGlu: 2.612 ± 0.366
1.348ProPhe: 1.348 ± 0.352
1.011ProGly: 1.011 ± 0.269
0.674ProHis: 0.674 ± 0.245
2.275ProIle: 2.275 ± 0.52
2.949ProLys: 2.949 ± 0.673
1.601ProLeu: 1.601 ± 0.438
0.674ProMet: 0.674 ± 0.195
1.264ProAsn: 1.264 ± 0.391
1.095ProPro: 1.095 ± 0.279
0.506ProGln: 0.506 ± 0.176
1.433ProArg: 1.433 ± 0.336
2.191ProSer: 2.191 ± 0.579
1.348ProThr: 1.348 ± 0.311
1.854ProVal: 1.854 ± 0.383
0.253ProTrp: 0.253 ± 0.154
0.843ProTyr: 0.843 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
3.371GlnAla: 3.371 ± 0.442
0.506GlnCys: 0.506 ± 0.222
2.107GlnAsp: 2.107 ± 0.465
2.781GlnGlu: 2.781 ± 0.486
0.927GlnPhe: 0.927 ± 0.238
2.191GlnGly: 2.191 ± 0.427
0.758GlnHis: 0.758 ± 0.299
2.612GlnIle: 2.612 ± 0.46
3.202GlnLys: 3.202 ± 0.529
3.202GlnLeu: 3.202 ± 0.515
0.674GlnMet: 0.674 ± 0.247
3.034GlnAsn: 3.034 ± 0.501
0.843GlnPro: 0.843 ± 0.224
1.854GlnGln: 1.854 ± 0.471
2.022GlnArg: 2.022 ± 0.371
1.938GlnSer: 1.938 ± 0.443
1.601GlnThr: 1.601 ± 0.365
2.191GlnVal: 2.191 ± 0.439
0.506GlnTrp: 0.506 ± 0.2
1.517GlnTyr: 1.517 ± 0.323
0.0GlnXaa: 0.0 ± 0.0
Arg
2.612ArgAla: 2.612 ± 0.497
0.169ArgCys: 0.169 ± 0.12
2.697ArgAsp: 2.697 ± 0.513
3.961ArgGlu: 3.961 ± 0.677
2.022ArgPhe: 2.022 ± 0.37
1.938ArgGly: 1.938 ± 0.448
0.506ArgHis: 0.506 ± 0.204
2.949ArgIle: 2.949 ± 0.385
3.876ArgLys: 3.876 ± 0.774
4.55ArgLeu: 4.55 ± 0.685
1.264ArgMet: 1.264 ± 0.25
2.781ArgAsn: 2.781 ± 0.439
0.59ArgPro: 0.59 ± 0.25
1.264ArgGln: 1.264 ± 0.35
2.275ArgArg: 2.275 ± 0.475
1.938ArgSer: 1.938 ± 0.434
2.697ArgThr: 2.697 ± 0.629
2.107ArgVal: 2.107 ± 0.395
0.59ArgTrp: 0.59 ± 0.22
2.697ArgTyr: 2.697 ± 0.648
0.0ArgXaa: 0.0 ± 0.0
Ser
4.129SerAla: 4.129 ± 0.476
0.253SerCys: 0.253 ± 0.14
4.129SerAsp: 4.129 ± 0.671
5.393SerGlu: 5.393 ± 0.782
2.697SerPhe: 2.697 ± 0.638
3.202SerGly: 3.202 ± 0.636
1.601SerHis: 1.601 ± 0.409
5.477SerIle: 5.477 ± 0.694
5.899SerLys: 5.899 ± 0.849
4.213SerLeu: 4.213 ± 0.738
1.348SerMet: 1.348 ± 0.32
4.55SerAsn: 4.55 ± 0.581
1.18SerPro: 1.18 ± 0.309
1.601SerGln: 1.601 ± 0.402
1.517SerArg: 1.517 ± 0.334
3.286SerSer: 3.286 ± 0.491
3.455SerThr: 3.455 ± 0.574
2.865SerVal: 2.865 ± 0.564
0.169SerTrp: 0.169 ± 0.111
2.275SerTyr: 2.275 ± 0.474
0.0SerXaa: 0.0 ± 0.0
Thr
3.876ThrAla: 3.876 ± 0.663
0.169ThrCys: 0.169 ± 0.115
3.371ThrAsp: 3.371 ± 0.903
3.371ThrGlu: 3.371 ± 0.499
1.854ThrPhe: 1.854 ± 0.333
3.623ThrGly: 3.623 ± 0.659
1.854ThrHis: 1.854 ± 0.412
4.129ThrIle: 4.129 ± 0.529
4.888ThrLys: 4.888 ± 0.604
4.972ThrLeu: 4.972 ± 0.5
0.927ThrMet: 0.927 ± 0.258
3.118ThrAsn: 3.118 ± 0.476
2.107ThrPro: 2.107 ± 0.315
2.528ThrGln: 2.528 ± 0.538
2.528ThrArg: 2.528 ± 0.453
3.961ThrSer: 3.961 ± 0.593
3.118ThrThr: 3.118 ± 0.667
3.034ThrVal: 3.034 ± 0.485
0.843ThrTrp: 0.843 ± 0.237
2.444ThrTyr: 2.444 ± 0.563
0.0ThrXaa: 0.0 ± 0.0
Val
2.949ValAla: 2.949 ± 0.521
0.169ValCys: 0.169 ± 0.115
3.202ValAsp: 3.202 ± 0.526
2.781ValGlu: 2.781 ± 0.537
2.359ValPhe: 2.359 ± 0.56
3.455ValGly: 3.455 ± 0.58
0.674ValHis: 0.674 ± 0.296
4.888ValIle: 4.888 ± 0.644
5.983ValLys: 5.983 ± 0.676
5.309ValLeu: 5.309 ± 0.574
1.517ValMet: 1.517 ± 0.304
4.466ValAsn: 4.466 ± 0.609
1.18ValPro: 1.18 ± 0.32
2.022ValGln: 2.022 ± 0.538
1.77ValArg: 1.77 ± 0.4
3.118ValSer: 3.118 ± 0.503
3.708ValThr: 3.708 ± 0.58
3.708ValVal: 3.708 ± 0.652
0.59ValTrp: 0.59 ± 0.237
2.781ValTyr: 2.781 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
0.337TrpAla: 0.337 ± 0.158
0.084TrpCys: 0.084 ± 0.084
0.674TrpAsp: 0.674 ± 0.223
0.927TrpGlu: 0.927 ± 0.239
0.674TrpPhe: 0.674 ± 0.264
0.506TrpGly: 0.506 ± 0.203
0.084TrpHis: 0.084 ± 0.084
1.264TrpIle: 1.264 ± 0.294
0.674TrpLys: 0.674 ± 0.231
1.264TrpLeu: 1.264 ± 0.343
0.337TrpMet: 0.337 ± 0.169
0.843TrpAsn: 0.843 ± 0.254
0.084TrpPro: 0.084 ± 0.073
0.59TrpGln: 0.59 ± 0.2
0.421TrpArg: 0.421 ± 0.188
0.758TrpSer: 0.758 ± 0.258
0.506TrpThr: 0.506 ± 0.208
0.59TrpVal: 0.59 ± 0.202
0.084TrpTrp: 0.084 ± 0.073
0.59TrpTyr: 0.59 ± 0.227
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.938TyrAla: 1.938 ± 0.465
0.169TyrCys: 0.169 ± 0.122
2.781TyrAsp: 2.781 ± 0.521
3.118TyrGlu: 3.118 ± 0.666
2.275TyrPhe: 2.275 ± 0.52
2.444TyrGly: 2.444 ± 0.531
0.674TyrHis: 0.674 ± 0.253
3.371TyrIle: 3.371 ± 0.689
4.888TyrLys: 4.888 ± 0.661
4.045TyrLeu: 4.045 ± 0.668
1.264TyrMet: 1.264 ± 0.31
2.022TyrAsn: 2.022 ± 0.352
1.011TyrPro: 1.011 ± 0.292
2.359TyrGln: 2.359 ± 0.483
1.77TyrArg: 1.77 ± 0.469
2.275TyrSer: 2.275 ± 0.461
2.022TyrThr: 2.022 ± 0.46
2.359TyrVal: 2.359 ± 0.406
0.421TyrTrp: 0.421 ± 0.215
1.18TyrTyr: 1.18 ± 0.39
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (11868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski