Ectothiorhodosinus mongolicus
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1835 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R3VX23|A0A1R3VX23_9GAMM Porphobilinogen deaminase OS=Ectothiorhodosinus mongolicus OX=233100 GN=hemC PE=3 SV=1
MM1 pKa = 7.33 LQINPNLVCWFVRR14 pKa = 11.84 HH15 pKa = 5.79 ARR17 pKa = 11.84 QFHH20 pKa = 5.3 AQEE23 pKa = 4.73 DD24 pKa = 4.36 VMLPDD29 pKa = 4.54 EE30 pKa = 5.49 PDD32 pKa = 3.55 PQNEE36 pKa = 4.25 GWVDD40 pKa = 3.42 EE41 pKa = 4.37 ALQEE45 pKa = 4.4 HH46 pKa = 7.08 SDD48 pKa = 3.44 NVLYY52 pKa = 11.07 LEE54 pKa = 5.11 LKK56 pKa = 10.64 DD57 pKa = 3.91 AVEE60 pKa = 3.95 EE61 pKa = 4.55 LEE63 pKa = 4.19 PQIQAEE69 pKa = 4.56 LVSLMWLGRR78 pKa = 11.84 GDD80 pKa = 3.77 YY81 pKa = 11.13 SEE83 pKa = 6.04 DD84 pKa = 3.27 EE85 pKa = 3.9 WDD87 pKa = 4.44 LALEE91 pKa = 4.59 DD92 pKa = 5.31 ALSNWTPRR100 pKa = 11.84 TADD103 pKa = 3.55 YY104 pKa = 11.51 LLATPLGADD113 pKa = 3.63 YY114 pKa = 11.1 LNEE117 pKa = 4.01 GLAMMGYY124 pKa = 9.67 SCDD127 pKa = 3.53 EE128 pKa = 4.55 DD129 pKa = 3.74 EE130 pKa = 4.84
Molecular weight: 14.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.49
ProMoST 3.821
Dawson 3.668
Bjellqvist 3.872
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.401
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.808
Patrickios 0.947
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A1R3VM99|A0A1R3VM99_9GAMM Uncharacterized protein OS=Ectothiorhodosinus mongolicus OX=233100 GN=SAMN05216526_0074 PE=4 SV=1
MM1 pKa = 7.53 SEE3 pKa = 3.88 YY4 pKa = 10.76 RR5 pKa = 11.84 FTKK8 pKa = 10.36 LAARR12 pKa = 11.84 DD13 pKa = 3.6 LEE15 pKa = 4.92 GIAKK19 pKa = 7.88 FTIEE23 pKa = 5.54 RR24 pKa = 11.84 FGLDD28 pKa = 2.73 QARR31 pKa = 11.84 RR32 pKa = 11.84 YY33 pKa = 9.83 RR34 pKa = 11.84 DD35 pKa = 3.38 EE36 pKa = 5.25 LRR38 pKa = 11.84 LCFEE42 pKa = 5.1 KK43 pKa = 10.82 LAEE46 pKa = 4.24 HH47 pKa = 6.76 PRR49 pKa = 11.84 LGQRR53 pKa = 11.84 AEE55 pKa = 3.95 HH56 pKa = 6.46 LGVGLRR62 pKa = 11.84 RR63 pKa = 11.84 HH64 pKa = 5.4 EE65 pKa = 4.67 HH66 pKa = 5.87 GSHH69 pKa = 4.63 IVFYY73 pKa = 9.65 KK74 pKa = 9.22 STGIGVLIARR84 pKa = 11.84 ILHH87 pKa = 5.9 RR88 pKa = 11.84 RR89 pKa = 11.84 MDD91 pKa = 3.25 ARR93 pKa = 11.84 GRR95 pKa = 11.84 LL96 pKa = 3.56
Molecular weight: 11.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.619
IPC_protein 10.643
Toseland 10.687
ProMoST 10.526
Dawson 10.774
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 10.818
Grimsley 10.833
Solomon 10.95
Lehninger 10.906
Nozaki 10.672
DTASelect 10.555
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.57
IPC_peptide 10.965
IPC2_peptide 9.663
IPC2.peptide.svr19 8.667
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1835
0
1835
627416
28
18099
341.9
37.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.74 ± 0.056
0.901 ± 0.039
5.707 ± 0.074
6.068 ± 0.133
3.517 ± 0.046
8.294 ± 0.274
2.336 ± 0.067
5.311 ± 0.054
2.934 ± 0.063
11.078 ± 0.139
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.546 ± 0.069
2.933 ± 0.139
4.65 ± 0.107
4.457 ± 0.082
6.743 ± 0.152
5.721 ± 0.1
5.151 ± 0.254
7.213 ± 0.046
1.313 ± 0.034
2.388 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here