Pelagibacterium lacus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Devosiaceae; Pelagibacterium

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369W4H4|A0A369W4H4_9RHIZ Aspartate/glutamate racemase family protein OS=Pelagibacterium lacus OX=2282655 GN=DVH29_05405 PE=3 SV=1
MM1 pKa = 7.21KK2 pKa = 9.63TLKK5 pKa = 9.69TLALAAAVSTAAAGGALAADD25 pKa = 5.49AIGVPAPIIPPAPVVDD41 pKa = 3.89MGSGFDD47 pKa = 3.4WNGFYY52 pKa = 10.66AGISGGVQNEE62 pKa = 4.36TVPGDD67 pKa = 3.68TSWALGAQAGVNSQFDD83 pKa = 3.84FFLVGAEE90 pKa = 3.93VSIEE94 pKa = 4.11GVFDD98 pKa = 5.34DD99 pKa = 5.09PDD101 pKa = 3.66TYY103 pKa = 11.53AYY105 pKa = 10.42GSALARR111 pKa = 11.84GGVLVTDD118 pKa = 3.84EE119 pKa = 4.44LLAYY123 pKa = 9.72GAIGYY128 pKa = 7.57GTDD131 pKa = 3.6FDD133 pKa = 5.04AATGPGDD140 pKa = 3.45HH141 pKa = 6.37VLAGGGLEE149 pKa = 4.43FAATDD154 pKa = 3.77DD155 pKa = 3.69VSVRR159 pKa = 11.84GQYY162 pKa = 10.8LYY164 pKa = 11.12GWEE167 pKa = 3.88QSGAAGASDD176 pKa = 3.27VHH178 pKa = 7.31KK179 pKa = 9.6FTIGANFHH187 pKa = 6.31FF188 pKa = 5.11

Molecular weight: 18.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369W9Q6|A0A369W9Q6_9RHIZ Taurine ABC transporter permease OS=Pelagibacterium lacus OX=2282655 GN=DVH29_03950 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVSGRR28 pKa = 11.84KK29 pKa = 8.9IINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84TKK37 pKa = 10.38GRR39 pKa = 11.84KK40 pKa = 8.19QLSAA44 pKa = 3.9

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3129

0

3129

967114

36

1883

309.1

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.801 ± 0.062

0.726 ± 0.012

5.814 ± 0.037

5.881 ± 0.038

3.783 ± 0.032

8.698 ± 0.039

2.013 ± 0.022

5.61 ± 0.032

2.706 ± 0.034

10.218 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.57 ± 0.022

2.522 ± 0.025

5.113 ± 0.034

3.051 ± 0.023

6.972 ± 0.042

5.286 ± 0.027

5.291 ± 0.026

7.448 ± 0.041

1.255 ± 0.018

2.242 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski