Armadillidium nasatum
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14427 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N5SRP6|A0A5N5SRP6_9CRUS Galectin (Fragment) OS=Armadillidium nasatum OX=96803 GN=LGALS7 PE=4 SV=1
MM1 pKa = 7.85 LLRR4 pKa = 11.84 NSSVTYY10 pKa = 10.4 CSEE13 pKa = 3.68 EE14 pKa = 4.3 GNWNDD19 pKa = 4.19 EE20 pKa = 4.01 GSKK23 pKa = 10.93 DD24 pKa = 3.65 STGVLQVIDD33 pKa = 4.23 WDD35 pKa = 5.41 PILLWYY41 pKa = 10.06 LPNLCCIKK49 pKa = 10.4 DD50 pKa = 3.44 IEE52 pKa = 4.32 IMVRR56 pKa = 11.84 GVGLNSISSIGCGSGLLEE74 pKa = 4.63 WIITSLTDD82 pKa = 3.46 ADD84 pKa = 4.17 ADD86 pKa = 3.82 ADD88 pKa = 3.94 ADD90 pKa = 4.05 ADD92 pKa = 3.85 TDD94 pKa = 3.81 ADD96 pKa = 3.78 ADD98 pKa = 3.77 ADD100 pKa = 3.97 ADD102 pKa = 3.94 AEE104 pKa = 4.73 AIPSIPADD112 pKa = 3.39 HH113 pKa = 7.13 ALLFCYY119 pKa = 10.4 FNDD122 pKa = 2.92 IVAFRR127 pKa = 11.84 SYY129 pKa = 11.01 LNKK132 pKa = 10.64 YY133 pKa = 10.15 KK134 pKa = 10.5 GDD136 pKa = 3.78 CLIIIGSVDD145 pKa = 3.1 GNTNDD150 pKa = 3.39 EE151 pKa = 4.33 SQSFGVEE158 pKa = 3.56 RR159 pKa = 11.84 VTT161 pKa = 4.94
Molecular weight: 17.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.643
IPC_protein 3.656
Toseland 3.427
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.63
Rodwell 3.478
Grimsley 3.338
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 4.062
Thurlkill 3.49
EMBOSS 3.643
Sillero 3.783
Patrickios 0.693
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A5N5SWM6|A0A5N5SWM6_9CRUS Neural cell adhesion molecule 2 (Fragment) OS=Armadillidium nasatum OX=96803 GN=Ncam2 PE=4 SV=1
GG1 pKa = 6.66 SAPVKK6 pKa = 10.77 HH7 pKa = 6.13 NVSTLNGSTSRR18 pKa = 11.84 LGASTSRR25 pKa = 11.84 SRR27 pKa = 11.84 SLSRR31 pKa = 11.84 LNTSGPSGIPNTKK44 pKa = 9.67 RR45 pKa = 11.84 SRR47 pKa = 11.84 TSSTTSLSSNHH58 pKa = 6.13 SASKK62 pKa = 10.6 IPILRR67 pKa = 3.9
Molecular weight: 6.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.471
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.457
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14427
0
14427
5254146
9
8334
364.2
41.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.966 ± 0.019
2.036 ± 0.016
5.314 ± 0.019
7.223 ± 0.031
4.334 ± 0.016
5.389 ± 0.03
2.425 ± 0.012
6.165 ± 0.018
7.293 ± 0.032
9.14 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.154 ± 0.01
5.448 ± 0.021
4.88 ± 0.028
3.727 ± 0.018
4.753 ± 0.017
9.125 ± 0.04
5.449 ± 0.022
5.893 ± 0.017
1.017 ± 0.008
3.272 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here