Spiribacter sp. 2438

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Spiribacter; unclassified Spiribacter

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1830 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B8JUZ3|A0A6B8JUZ3_9GAMM D-amino-acid transaminase OS=Spiribacter sp. 2438 OX=2666185 GN=GJ672_07040 PE=4 SV=1
MM1 pKa = 7.47SNRR4 pKa = 11.84TSRR7 pKa = 11.84MLLTGALGLAMAGGASAQDD26 pKa = 3.5DD27 pKa = 4.33TIEE30 pKa = 4.48FGWTAWSDD38 pKa = 3.66AEE40 pKa = 4.62FMTRR44 pKa = 11.84LAAQLVNDD52 pKa = 4.07HH53 pKa = 6.64TDD55 pKa = 2.98YY56 pKa = 11.26SADD59 pKa = 3.44MVQTDD64 pKa = 4.53IAPQFQGLATGDD76 pKa = 3.26IDD78 pKa = 4.71AMLMAWLPATHH89 pKa = 6.59EE90 pKa = 4.13EE91 pKa = 4.88YY92 pKa = 11.16YY93 pKa = 10.78EE94 pKa = 4.48GVADD98 pKa = 4.07DD99 pKa = 4.28VEE101 pKa = 4.48NLGVLYY107 pKa = 10.92NSARR111 pKa = 11.84LGWAVPTYY119 pKa = 10.77VPEE122 pKa = 4.6DD123 pKa = 3.51QLSSIEE129 pKa = 4.75DD130 pKa = 3.56LTDD133 pKa = 4.23DD134 pKa = 4.47SVQAEE139 pKa = 4.45LGGTITGIDD148 pKa = 3.65PGAGLTSLSEE158 pKa = 4.2QALEE162 pKa = 4.37TYY164 pKa = 10.84GLDD167 pKa = 4.52DD168 pKa = 3.7YY169 pKa = 11.4TLQTSSGAGMTAALDD184 pKa = 3.79RR185 pKa = 11.84AVGRR189 pKa = 11.84DD190 pKa = 3.14DD191 pKa = 4.21WIVVTGWSPHH201 pKa = 4.49WKK203 pKa = 9.81FGAYY207 pKa = 7.84EE208 pKa = 3.93LRR210 pKa = 11.84YY211 pKa = 10.44LEE213 pKa = 4.95DD214 pKa = 3.95PEE216 pKa = 4.75GALGGPEE223 pKa = 3.59RR224 pKa = 11.84VHH226 pKa = 7.2ALGRR230 pKa = 11.84AGFDD234 pKa = 2.87ADD236 pKa = 3.7YY237 pKa = 11.24PEE239 pKa = 4.71ISEE242 pKa = 4.34MLSRR246 pKa = 11.84MYY248 pKa = 11.01VPIDD252 pKa = 3.4EE253 pKa = 4.89LQDD256 pKa = 3.54YY257 pKa = 8.27MFEE260 pKa = 4.05ASEE263 pKa = 4.33TSFEE267 pKa = 4.34EE268 pKa = 4.23AVSAYY273 pKa = 9.7IDD275 pKa = 4.38DD276 pKa = 4.17NPDD279 pKa = 4.05RR280 pKa = 11.84IQYY283 pKa = 8.7WLTGEE288 pKa = 4.16LL289 pKa = 3.79

Molecular weight: 31.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B8JW32|A0A6B8JW32_9GAMM Urea ABC transporter permease subunit UrtC OS=Spiribacter sp. 2438 OX=2666185 GN=urtC PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.5RR12 pKa = 11.84KK13 pKa = 9.44RR14 pKa = 11.84NHH16 pKa = 5.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.79ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84ARR41 pKa = 11.84LTPP44 pKa = 3.93

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1830

0

1830

596256

38

1835

325.8

35.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.701 ± 0.068

0.876 ± 0.02

6.037 ± 0.049

6.461 ± 0.055

3.264 ± 0.032

8.701 ± 0.044

2.357 ± 0.027

4.646 ± 0.044

1.93 ± 0.038

10.903 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.393 ± 0.025

2.355 ± 0.027

5.281 ± 0.038

3.681 ± 0.032

8.229 ± 0.054

5.043 ± 0.034

4.893 ± 0.03

7.631 ± 0.047

1.402 ± 0.029

2.216 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski