Acidomonas methanolica NBRC 104435

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acidomonas; Acidomonas methanolica

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023D805|A0A023D805_ACIMT Biotin synthase OS=Acidomonas methanolica NBRC 104435 OX=1231351 GN=bioB PE=3 SV=1
MM1 pKa = 7.55SIFGAINTAVSGLDD15 pKa = 3.41AQSKK19 pKa = 10.34AFSNLSNNIANSQTTGYY36 pKa = 10.33KK37 pKa = 10.14ADD39 pKa = 3.4TTAFEE44 pKa = 4.5NYY46 pKa = 7.04VTGDD50 pKa = 3.35EE51 pKa = 4.57DD52 pKa = 4.44FISAVGGDD60 pKa = 4.06DD61 pKa = 3.89SIKK64 pKa = 11.21AMTIQHH70 pKa = 6.58NDD72 pKa = 2.9VQGAITSSTNSLAVAISGNGMFNVLQATGGANSASPVYY110 pKa = 10.49NSQQYY115 pKa = 6.64YY116 pKa = 8.36TRR118 pKa = 11.84NGDD121 pKa = 3.37FSVNANGYY129 pKa = 9.1LVNTSGDD136 pKa = 3.55FLEE139 pKa = 5.95GYY141 pKa = 10.35AVGSTGSVAGTAAPIQVTGLNTPSPTTTLTLNAAIGDD178 pKa = 4.03TAGDD182 pKa = 3.51VSTNTSTATAYY193 pKa = 9.33DD194 pKa = 3.38ASGNAVPVTLDD205 pKa = 3.06WAQSGSNPLDD215 pKa = 3.14WTVGVAGSGTTTPVTFNSDD234 pKa = 2.62GSLASVGGSTSSGNASITVPATSTTEE260 pKa = 3.77ARR262 pKa = 11.84AMTLDD267 pKa = 3.87LGTIGSSNGVNLASSSAGVTSNPIMTTDD295 pKa = 2.98SKK297 pKa = 10.13TPTGVTITSAGLVEE311 pKa = 4.38KK312 pKa = 9.49TYY314 pKa = 11.48SDD316 pKa = 3.7GTTDD320 pKa = 3.37PVAQIKK326 pKa = 10.31LAMFTNANGLASTDD340 pKa = 3.56GQNYY344 pKa = 6.64TATPASGPVTYY355 pKa = 8.49EE356 pKa = 3.85TVGAGAAGTLDD367 pKa = 3.37TSSIEE372 pKa = 4.33GSTTDD377 pKa = 3.86LTGDD381 pKa = 4.24LSNLIVAQQAYY392 pKa = 8.94GANTKK397 pKa = 9.9VVTTADD403 pKa = 3.13QLMQTTIAMIQQ414 pKa = 3.01

Molecular weight: 41.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023D0E6|A0A023D0E6_ACIMT Methionine aminopeptidase OS=Acidomonas methanolica NBRC 104435 OX=1231351 GN=map PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.02RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.44GFRR19 pKa = 11.84TRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.35VIANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.23GRR39 pKa = 11.84HH40 pKa = 5.48KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3270

0

3270

1036497

41

1747

317.0

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.75 ± 0.058

0.915 ± 0.013

5.662 ± 0.033

5.304 ± 0.045

3.526 ± 0.027

8.692 ± 0.045

2.359 ± 0.022

4.743 ± 0.03

2.418 ± 0.03

10.474 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.018

2.376 ± 0.03

5.747 ± 0.035

2.991 ± 0.026

7.99 ± 0.048

5.343 ± 0.034

5.467 ± 0.033

7.185 ± 0.035

1.434 ± 0.017

2.206 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski