Trichoderma longibrachiatum ATCC 18648
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10930 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T4C3Y8|A0A2T4C3Y8_TRILO Branched-chain-amino-acid aminotransferase OS=Trichoderma longibrachiatum ATCC 18648 OX=983965 GN=M440DRAFT_1438976 PE=3 SV=1
MM1 pKa = 7.24 QFLTAAFALLGLALTASAADD21 pKa = 3.77 EE22 pKa = 4.1 QLCFPVPGQINNVPQSITDD41 pKa = 3.76 LDD43 pKa = 4.06 VQIKK47 pKa = 9.06 IHH49 pKa = 6.41 WATKK53 pKa = 9.48 LCAQIDD59 pKa = 4.58 YY60 pKa = 9.06 STVDD64 pKa = 3.49 AQSVTTDD71 pKa = 2.95 VADD74 pKa = 4.38 GVDD77 pKa = 3.1 ATEE80 pKa = 3.99 NGKK83 pKa = 8.0 TYY85 pKa = 10.82 GLNLVTVAVPNEE97 pKa = 4.15 EE98 pKa = 4.0 KK99 pKa = 10.97 CIDD102 pKa = 3.58 NAAATLGADD111 pKa = 3.54 VCPSGGAFINLDD123 pKa = 3.63 NNEE126 pKa = 4.47 EE127 pKa = 3.99 EE128 pKa = 4.32 WFSIVALDD136 pKa = 3.48
Molecular weight: 14.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.643
IPC_protein 3.605
Toseland 3.389
ProMoST 3.732
Dawson 3.617
Bjellqvist 3.821
Wikipedia 3.579
Rodwell 3.439
Grimsley 3.312
Solomon 3.592
Lehninger 3.541
Nozaki 3.745
DTASelect 3.961
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.732
Patrickios 0.769
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|A0A2T4C8X6|A0A2T4C8X6_TRILO Kinase-like protein OS=Trichoderma longibrachiatum ATCC 18648 OX=983965 GN=M440DRAFT_1328958 PE=4 SV=1
LL1 pKa = 6.2 THH3 pKa = 5.54 THH5 pKa = 5.8 SRR7 pKa = 11.84 SHH9 pKa = 6.36 TPAQPGIQSQYY20 pKa = 9.84 LFSGFVFNARR30 pKa = 11.84 LPSSSFLSNSFSLLFFFFFFLSQASSSSSPQFVLILVLVLVRR72 pKa = 11.84 PSVRR76 pKa = 11.84 LSSLFPAPLANRR88 pKa = 11.84 PSTVRR93 pKa = 11.84 LSQPPSIRR101 pKa = 11.84 VSPRR105 pKa = 11.84 PRR107 pKa = 11.84 QLINRR112 pKa = 11.84 RR113 pKa = 11.84 KK114 pKa = 9.87 QKK116 pKa = 9.85 TII118 pKa = 3.2
Molecular weight: 13.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.424
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.237
Grimsley 12.691
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.974
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.109
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10929
1
10930
4672678
49
23089
427.5
47.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.159 ± 0.022
1.347 ± 0.011
5.723 ± 0.017
6.094 ± 0.026
3.655 ± 0.015
6.864 ± 0.025
2.436 ± 0.012
4.635 ± 0.016
4.731 ± 0.024
9.008 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.217 ± 0.01
3.383 ± 0.012
6.14 ± 0.023
4.044 ± 0.016
6.439 ± 0.024
8.239 ± 0.026
5.655 ± 0.016
6.098 ± 0.02
1.476 ± 0.009
2.64 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here