Synechococcus virus P60
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8W6Y6|Q8W6Y6_9CAUD Uncharacterized protein OS=Synechococcus virus P60 OX=151528 GN=P60_gp01 PE=4 SV=1
MM1 pKa = 7.92 TDD3 pKa = 2.94 TTIYY7 pKa = 9.95 QLPVHH12 pKa = 6.83 WAPALINDD20 pKa = 4.05 DD21 pKa = 3.29 WTGLEE26 pKa = 4.17 EE27 pKa = 4.22 QDD29 pKa = 4.65 EE30 pKa = 4.49 EE31 pKa = 4.47 QLMAFLFNEE40 pKa = 4.2 LAGEE44 pKa = 4.73 GVCITLDD51 pKa = 3.52 PEE53 pKa = 4.14 EE54 pKa = 4.88 EE55 pKa = 4.14 PFFMKK60 pKa = 10.44 HH61 pKa = 5.73 HH62 pKa = 6.8 DD63 pKa = 3.77 AKK65 pKa = 10.91 PYY67 pKa = 10.62 GCLACDD73 pKa = 4.24 CYY75 pKa = 11.38 NYY77 pKa = 10.31 TVIDD81 pKa = 3.82 MNN83 pKa = 4.28
Molecular weight: 9.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.885
IPC2_protein 3.834
IPC_protein 3.757
Toseland 3.567
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.872
Patrickios 0.083
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|Q8W6W8|Q8W6W8_9CAUD Uncharacterized protein OS=Synechococcus virus P60 OX=151528 GN=P60_gp44 PE=4 SV=1
MM1 pKa = 7.72 APRR4 pKa = 11.84 RR5 pKa = 11.84 TSNPGKK11 pKa = 8.65 SARR14 pKa = 11.84 YY15 pKa = 8.9 YY16 pKa = 10.47 RR17 pKa = 11.84 SNPKK21 pKa = 10.01 ARR23 pKa = 11.84 AKK25 pKa = 10.57 KK26 pKa = 9.88 NAAQRR31 pKa = 11.84 KK32 pKa = 8.03 RR33 pKa = 11.84 NKK35 pKa = 7.38 TTAAKK40 pKa = 10.18 KK41 pKa = 10.24 YY42 pKa = 9.61 RR43 pKa = 11.84 AEE45 pKa = 3.88 LNAARR50 pKa = 11.84 RR51 pKa = 11.84 KK52 pKa = 10.05 DD53 pKa = 3.45 GNYY56 pKa = 10.37 GKK58 pKa = 10.66 GGKK61 pKa = 9.64 DD62 pKa = 3.33 YY63 pKa = 11.63 SHH65 pKa = 6.78 TKK67 pKa = 9.48 SGKK70 pKa = 9.18 LVRR73 pKa = 11.84 EE74 pKa = 3.9 SPKK77 pKa = 10.4 RR78 pKa = 11.84 NRR80 pKa = 11.84 ARR82 pKa = 11.84 QGAGGRR88 pKa = 11.84 PKK90 pKa = 10.48 RR91 pKa = 11.84 KK92 pKa = 9.62
Molecular weight: 10.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.18
IPC2_protein 10.584
IPC_protein 11.696
Toseland 11.857
ProMoST 12.281
Dawson 11.857
Bjellqvist 11.813
Wikipedia 12.296
Rodwell 11.842
Grimsley 11.901
Solomon 12.31
Lehninger 12.223
Nozaki 11.842
DTASelect 11.813
Thurlkill 11.842
EMBOSS 12.34
Sillero 11.842
Patrickios 11.564
IPC_peptide 12.31
IPC2_peptide 11.272
IPC2.peptide.svr19 8.896
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
14503
42
1639
263.7
29.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.77 ± 0.545
0.807 ± 0.157
6.592 ± 0.256
6.371 ± 0.369
3.572 ± 0.182
7.916 ± 0.586
1.586 ± 0.193
4.751 ± 0.181
4.992 ± 0.308
8.191 ± 0.338
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.303 ± 0.177
4.544 ± 0.311
4.571 ± 0.244
4.964 ± 0.313
5.461 ± 0.326
6.213 ± 0.242
6.419 ± 0.38
6.109 ± 0.278
1.434 ± 0.146
3.434 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here