Mesorhizobium sp. Root157

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3925 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8AFI2|A0A0Q8AFI2_9RHIZ ATPase OS=Mesorhizobium sp. Root157 OX=1736477 GN=ASD64_10455 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 5.04KK3 pKa = 9.88VTDD6 pKa = 4.55SIAHH10 pKa = 6.64FIGLFQIAAEE20 pKa = 4.12QARR23 pKa = 11.84MRR25 pKa = 11.84DD26 pKa = 3.54DD27 pKa = 3.37YY28 pKa = 11.93LEE30 pKa = 4.32FVARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 7.28DD38 pKa = 3.64EE39 pKa = 4.39APPSDD44 pKa = 4.04EE45 pKa = 3.81PVQIKK50 pKa = 9.98FDD52 pKa = 3.57APYY55 pKa = 11.19ALDD58 pKa = 3.7NPEE61 pKa = 4.41PGIHH65 pKa = 5.88YY66 pKa = 10.44VPAPNSIEE74 pKa = 4.23PYY76 pKa = 8.55VAHH79 pKa = 7.13HH80 pKa = 6.72EE81 pKa = 4.11PLYY84 pKa = 10.38AAPEE88 pKa = 4.15IPISAQMQPVIYY100 pKa = 8.94PGYY103 pKa = 8.08VPPVFLHH110 pKa = 6.3HH111 pKa = 6.94ASSSLKK117 pKa = 10.4IIDD120 pKa = 4.05PQPPGSVASIVSQTNHH136 pKa = 6.64LSDD139 pKa = 3.61NDD141 pKa = 3.65FVNVGGSDD149 pKa = 3.25AVFEE153 pKa = 4.72QIGTPQTSLQEE164 pKa = 4.42LVDD167 pKa = 3.88QASQAMPILAGSLPGSSQDD186 pKa = 2.46IGTFITTSADD196 pKa = 3.65SIHH199 pKa = 7.3DD200 pKa = 3.72FLAGLEE206 pKa = 4.25SGQQAGEE213 pKa = 4.23AGSSQTIVDD222 pKa = 4.04NGDD225 pKa = 3.4GTSTATQVTAVTPAEE240 pKa = 3.9DD241 pKa = 3.21TVYY244 pKa = 11.32VNGTATDD251 pKa = 3.86EE252 pKa = 4.13APKK255 pKa = 10.64LDD257 pKa = 4.99DD258 pKa = 4.21YY259 pKa = 11.8LPAASPLVEE268 pKa = 4.4DD269 pKa = 5.06TPPEE273 pKa = 4.21AAPEE277 pKa = 4.02ASNGSWQTGSTATGQVVHH295 pKa = 6.35GQGSIAVTASVEE307 pKa = 3.79ISTGLNVLVNSATLVSDD324 pKa = 3.38TLEE327 pKa = 3.94GHH329 pKa = 6.15VFAIAGDD336 pKa = 4.33HH337 pKa = 6.58ISLNLIVQINAWSDD351 pKa = 3.22ADD353 pKa = 3.88SVGASMSGWNCPPDD367 pKa = 3.36GTTAFNIASMEE378 pKa = 4.04HH379 pKa = 6.18VEE381 pKa = 4.76LNTGTDD387 pKa = 3.63EE388 pKa = 4.4PSGAGTPVFPKK399 pKa = 9.8AWAVTEE405 pKa = 3.92ITGDD409 pKa = 4.33FISLNWLQQLNFVIDD424 pKa = 3.65NDD426 pKa = 4.32TVVASSSAGVTSMVGTGANQTFNSLSIFDD455 pKa = 3.93LGKK458 pKa = 10.71YY459 pKa = 10.0FDD461 pKa = 5.93LILIGGNYY469 pKa = 9.29YY470 pKa = 10.07NANIILQKK478 pKa = 11.08NIMLDD483 pKa = 3.51DD484 pKa = 5.51DD485 pKa = 3.94IVGGVSGFQTSGPGAVSTSDD505 pKa = 3.15NLLWNEE511 pKa = 4.1ARR513 pKa = 11.84ITNIGTANTDD523 pKa = 3.2GMPDD527 pKa = 3.48GFHH530 pKa = 6.64NALNEE535 pKa = 3.8FAAGNKK541 pKa = 9.01VLSADD546 pKa = 3.88VLNDD550 pKa = 3.29SAFAGLAGLHH560 pKa = 5.61VLYY563 pKa = 10.29ISGSIYY569 pKa = 10.41DD570 pKa = 3.92LQYY573 pKa = 10.64IQQTNILGDD582 pKa = 3.91ADD584 pKa = 3.66QVAAAMQGVNPTSDD598 pKa = 4.15ANWSITTGSNVLVNQAQIIDD618 pKa = 4.08VGPGGAIYY626 pKa = 10.56YY627 pKa = 10.7GGGQYY632 pKa = 10.51SDD634 pKa = 4.14EE635 pKa = 4.99LLVQTDD641 pKa = 4.53IIRR644 pKa = 11.84TDD646 pKa = 2.81NGTDD650 pKa = 3.21VKK652 pKa = 11.44SPDD655 pKa = 3.4QLVNEE660 pKa = 4.55AVAFLSDD667 pKa = 5.55DD668 pKa = 3.75MLTPDD673 pKa = 4.44QSQEE677 pKa = 3.73HH678 pKa = 4.98QMGIKK683 pKa = 10.06DD684 pKa = 4.1DD685 pKa = 4.13VGMHH689 pKa = 6.8PAHH692 pKa = 6.59TDD694 pKa = 2.51IMQSVVTT701 pKa = 4.53

Molecular weight: 73.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8AWM5|A0A0Q8AWM5_9RHIZ Myo-inosose-2 dehydratase OS=Mesorhizobium sp. Root157 OX=1736477 GN=ASD64_02730 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.14GGRR28 pKa = 11.84SVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84QRR41 pKa = 11.84LSAA44 pKa = 4.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3925

0

3925

1190505

41

4699

303.3

32.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.313 ± 0.056

0.788 ± 0.012

5.755 ± 0.035

5.792 ± 0.04

3.86 ± 0.031

8.552 ± 0.047

2.011 ± 0.018

5.427 ± 0.029

3.872 ± 0.033

9.725 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.019

2.762 ± 0.026

4.875 ± 0.027

3.092 ± 0.023

6.758 ± 0.045

5.555 ± 0.03

5.253 ± 0.035

7.5 ± 0.029

1.287 ± 0.017

2.256 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski