Amino acid dipepetide frequency for Mesorhizobium sp. Root157

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.658AlaAla: 16.658 ± 0.196	0.959AlaCys: 0.959 ± 0.03	6.973AlaAsp: 6.973 ± 0.079	7.639AlaGlu: 7.639 ± 0.082	4.732AlaPhe: 4.732 ± 0.065	10.742AlaGly: 10.742 ± 0.115	2.268AlaHis: 2.268 ± 0.051	6.774AlaIle: 6.774 ± 0.089	4.774AlaLys: 4.774 ± 0.081	12.48AlaLeu: 12.48 ± 0.121	3.577AlaMet: 3.577 ± 0.052	3.247AlaAsn: 3.247 ± 0.053	4.955AlaPro: 4.955 ± 0.095	3.563AlaGln: 3.563 ± 0.061	8.055AlaArg: 8.055 ± 0.093	6.774AlaSer: 6.774 ± 0.086	5.968AlaThr: 5.968 ± 0.081	8.861AlaVal: 8.861 ± 0.086	1.452AlaTrp: 1.452 ± 0.041	2.682AlaTyr: 2.682 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.855CysAla: 0.855 ± 0.022	0.089CysCys: 0.089 ± 0.009	0.507CysAsp: 0.507 ± 0.021	0.423CysGlu: 0.423 ± 0.019	0.342CysPhe: 0.342 ± 0.019	0.881CysGly: 0.881 ± 0.028	0.221CysHis: 0.221 ± 0.014	0.363CysIle: 0.363 ± 0.02	0.209CysLys: 0.209 ± 0.014	0.74CysLeu: 0.74 ± 0.026	0.154CysMet: 0.154 ± 0.01	0.198CysAsn: 0.198 ± 0.014	0.391CysPro: 0.391 ± 0.021	0.193CysGln: 0.193 ± 0.012	0.552CysArg: 0.552 ± 0.022	0.458CysSer: 0.458 ± 0.02	0.4CysThr: 0.4 ± 0.021	0.567CysVal: 0.567 ± 0.023	0.118CysTrp: 0.118 ± 0.01	0.215CysTyr: 0.215 ± 0.017	0.001CysXaa: 0.001 ± 0.001
Asp	6.83AspAla: 6.83 ± 0.087	0.481AspCys: 0.481 ± 0.02	3.252AspAsp: 3.252 ± 0.066	3.592AspGlu: 3.592 ± 0.067	2.226AspPhe: 2.226 ± 0.045	5.211AspGly: 5.211 ± 0.099	1.269AspHis: 1.269 ± 0.034	3.404AspIle: 3.404 ± 0.054	2.128AspLys: 2.128 ± 0.044	5.724AspLeu: 5.724 ± 0.08	1.443AspMet: 1.443 ± 0.034	1.536AspAsn: 1.536 ± 0.037	3.201AspPro: 3.201 ± 0.05	1.74AspGln: 1.74 ± 0.037	4.169AspArg: 4.169 ± 0.06	2.168AspSer: 2.168 ± 0.043	2.688AspThr: 2.688 ± 0.054	4.117AspVal: 4.117 ± 0.063	0.919AspTrp: 0.919 ± 0.026	1.452AspTyr: 1.452 ± 0.033	0.001AspXaa: 0.001 ± 0.001
Glu	7.797GluAla: 7.797 ± 0.116	0.34GluCys: 0.34 ± 0.018	2.725GluAsp: 2.725 ± 0.051	3.132GluGlu: 3.132 ± 0.054	1.988GluPhe: 1.988 ± 0.038	4.188GluGly: 4.188 ± 0.059	1.236GluHis: 1.236 ± 0.037	3.71GluIle: 3.71 ± 0.061	2.737GluLys: 2.737 ± 0.059	5.273GluLeu: 5.273 ± 0.074	1.639GluMet: 1.639 ± 0.041	1.737GluAsn: 1.737 ± 0.041	2.748GluPro: 2.748 ± 0.056	2.264GluGln: 2.264 ± 0.047	5.036GluArg: 5.036 ± 0.082	2.386GluSer: 2.386 ± 0.049	3.478GluThr: 3.478 ± 0.054	3.81GluVal: 3.81 ± 0.056	0.713GluTrp: 0.713 ± 0.025	0.982GluTyr: 0.982 ± 0.028	0.002GluXaa: 0.002 ± 0.001
Phe	4.658PheAla: 4.658 ± 0.072	0.396PheCys: 0.396 ± 0.018	2.733PheAsp: 2.733 ± 0.052	2.312PheGlu: 2.312 ± 0.045	1.518PhePhe: 1.518 ± 0.042	3.851PheGly: 3.851 ± 0.056	0.779PheHis: 0.779 ± 0.027	1.855PheIle: 1.855 ± 0.041	1.178PheLys: 1.178 ± 0.028	3.427PheLeu: 3.427 ± 0.063	0.867PheMet: 0.867 ± 0.029	1.092PheAsn: 1.092 ± 0.032	1.626PhePro: 1.626 ± 0.039	1.131PheGln: 1.131 ± 0.03	2.206PheArg: 2.206 ± 0.045	2.495PheSer: 2.495 ± 0.05	1.929PheThr: 1.929 ± 0.045	3.019PheVal: 3.019 ± 0.054	0.558PheTrp: 0.558 ± 0.026	0.975PheTyr: 0.975 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	8.93GlyAla: 8.93 ± 0.119	0.786GlyCys: 0.786 ± 0.026	4.527GlyAsp: 4.527 ± 0.072	4.995GlyGlu: 4.995 ± 0.064	3.734GlyPhe: 3.734 ± 0.057	7.152GlyGly: 7.152 ± 0.12	1.853GlyHis: 1.853 ± 0.04	5.032GlyIle: 5.032 ± 0.067	4.125GlyLys: 4.125 ± 0.068	8.586GlyLeu: 8.586 ± 0.104	2.377GlyMet: 2.377 ± 0.045	2.355GlyAsn: 2.355 ± 0.053	3.315GlyPro: 3.315 ± 0.062	2.789GlyGln: 2.789 ± 0.05	5.824GlyArg: 5.824 ± 0.08	4.821GlySer: 4.821 ± 0.084	4.657GlyThr: 4.657 ± 0.072	6.071GlyVal: 6.071 ± 0.077	1.331GlyTrp: 1.331 ± 0.032	2.256GlyTyr: 2.256 ± 0.047	0.001GlyXaa: 0.001 ± 0.001
His	2.288HisAla: 2.288 ± 0.044	0.224HisCys: 0.224 ± 0.015	1.269HisAsp: 1.269 ± 0.029	1.084HisGlu: 1.084 ± 0.029	0.905HisPhe: 0.905 ± 0.031	1.936HisGly: 1.936 ± 0.042	0.534HisHis: 0.534 ± 0.022	1.011HisIle: 1.011 ± 0.032	0.582HisLys: 0.582 ± 0.022	1.904HisLeu: 1.904 ± 0.046	0.512HisMet: 0.512 ± 0.018	0.52HisAsn: 0.52 ± 0.018	1.21HisPro: 1.21 ± 0.032	0.585HisGln: 0.585 ± 0.018	1.366HisArg: 1.366 ± 0.037	0.975HisSer: 0.975 ± 0.032	0.841HisThr: 0.841 ± 0.029	1.514HisVal: 1.514 ± 0.037	0.316HisTrp: 0.316 ± 0.017	0.533HisTyr: 0.533 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.465IleAla: 7.465 ± 0.086	0.491IleCys: 0.491 ± 0.021	3.879IleAsp: 3.879 ± 0.067	3.856IleGlu: 3.856 ± 0.064	1.856IlePhe: 1.856 ± 0.048	5.177IleGly: 5.177 ± 0.074	0.989IleHis: 0.989 ± 0.031	2.494IleIle: 2.494 ± 0.051	1.664IleLys: 1.664 ± 0.04	4.575IleLeu: 4.575 ± 0.074	1.084IleMet: 1.084 ± 0.028	1.514IleAsn: 1.514 ± 0.037	2.3IlePro: 2.3 ± 0.041	1.215IleGln: 1.215 ± 0.032	3.362IleArg: 3.362 ± 0.056	3.215IleSer: 3.215 ± 0.047	2.639IleThr: 2.639 ± 0.048	4.679IleVal: 4.679 ± 0.069	0.602IleTrp: 0.602 ± 0.024	1.212IleTyr: 1.212 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	5.199LysAla: 5.199 ± 0.086	0.176LysCys: 0.176 ± 0.013	1.969LysAsp: 1.969 ± 0.043	1.926LysGlu: 1.926 ± 0.052	1.047LysPhe: 1.047 ± 0.029	3.11LysGly: 3.11 ± 0.056	0.659LysHis: 0.659 ± 0.024	2.06LysIle: 2.06 ± 0.045	1.696LysLys: 1.696 ± 0.051	3.878LysLeu: 3.878 ± 0.062	0.927LysMet: 0.927 ± 0.029	1.054LysAsn: 1.054 ± 0.032	2.428LysPro: 2.428 ± 0.049	1.225LysGln: 1.225 ± 0.034	2.787LysArg: 2.787 ± 0.054	2.155LysSer: 2.155 ± 0.045	2.291LysThr: 2.291 ± 0.047	2.992LysVal: 2.992 ± 0.056	0.406LysTrp: 0.406 ± 0.022	0.732LysTyr: 0.732 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.034LeuAla: 13.034 ± 0.121	0.822LeuCys: 0.822 ± 0.025	5.843LeuAsp: 5.843 ± 0.069	5.222LeuGlu: 5.222 ± 0.071	3.693LeuPhe: 3.693 ± 0.061	7.974LeuGly: 7.974 ± 0.102	1.741LeuHis: 1.741 ± 0.043	5.028LeuIle: 5.028 ± 0.082	4.128LeuLys: 4.128 ± 0.063	8.806LeuLeu: 8.806 ± 0.111	2.323LeuMet: 2.323 ± 0.047	2.604LeuAsn: 2.604 ± 0.047	5.118LeuPro: 5.118 ± 0.08	2.608LeuGln: 2.608 ± 0.05	6.03LeuArg: 6.03 ± 0.081	6.324LeuSer: 6.324 ± 0.073	5.197LeuThr: 5.197 ± 0.069	7.577LeuVal: 7.577 ± 0.096	1.06LeuTrp: 1.06 ± 0.03	2.12LeuTyr: 2.12 ± 0.045	0.001LeuXaa: 0.001 ± 0.001
Met	3.356MetAla: 3.356 ± 0.047	0.167MetCys: 0.167 ± 0.013	1.227MetAsp: 1.227 ± 0.032	1.16MetGlu: 1.16 ± 0.03	0.713MetPhe: 0.713 ± 0.029	1.861MetGly: 1.861 ± 0.042	0.456MetHis: 0.456 ± 0.019	1.379MetIle: 1.379 ± 0.036	1.132MetLys: 1.132 ± 0.03	2.632MetLeu: 2.632 ± 0.049	0.706MetMet: 0.706 ± 0.028	0.841MetAsn: 0.841 ± 0.027	1.495MetPro: 1.495 ± 0.034	0.865MetGln: 0.865 ± 0.025	1.808MetArg: 1.808 ± 0.043	1.636MetSer: 1.636 ± 0.035	1.851MetThr: 1.851 ± 0.042	1.823MetVal: 1.823 ± 0.044	0.213MetTrp: 0.213 ± 0.013	0.325MetTyr: 0.325 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.351AsnAla: 3.351 ± 0.047	0.236AsnCys: 0.236 ± 0.015	1.543AsnAsp: 1.543 ± 0.038	1.41AsnGlu: 1.41 ± 0.034	1.026AsnPhe: 1.026 ± 0.027	2.695AsnGly: 2.695 ± 0.064	0.537AsnHis: 0.537 ± 0.022	1.483AsnIle: 1.483 ± 0.039	0.873AsnLys: 0.873 ± 0.029	2.532AsnLeu: 2.532 ± 0.051	0.687AsnMet: 0.687 ± 0.023	0.721AsnAsn: 0.721 ± 0.028	1.924AsnPro: 1.924 ± 0.042	0.793AsnGln: 0.793 ± 0.028	1.856AsnArg: 1.856 ± 0.04	1.393AsnSer: 1.393 ± 0.037	1.334AsnThr: 1.334 ± 0.038	2.051AsnVal: 2.051 ± 0.044	0.47AsnTrp: 0.47 ± 0.02	0.706AsnTyr: 0.706 ± 0.028	0.001AsnXaa: 0.001 ± 0.001
Pro	5.978ProAla: 5.978 ± 0.087	0.274ProCys: 0.274 ± 0.015	3.43ProAsp: 3.43 ± 0.058	3.384ProGlu: 3.384 ± 0.065	1.944ProPhe: 1.944 ± 0.043	4.203ProGly: 4.203 ± 0.062	0.985ProHis: 0.985 ± 0.029	2.286ProIle: 2.286 ± 0.044	1.882ProLys: 1.882 ± 0.045	4.518ProLeu: 4.518 ± 0.067	1.1ProMet: 1.1 ± 0.03	1.358ProAsn: 1.358 ± 0.036	2.096ProPro: 2.096 ± 0.048	1.725ProGln: 1.725 ± 0.04	2.586ProArg: 2.586 ± 0.054	2.7ProSer: 2.7 ± 0.045	2.344ProThr: 2.344 ± 0.047	4.118ProVal: 4.118 ± 0.066	0.634ProTrp: 0.634 ± 0.025	1.206ProTyr: 1.206 ± 0.034	0.002ProXaa: 0.002 ± 0.001
Gln	3.952GlnAla: 3.952 ± 0.063	0.202GlnCys: 0.202 ± 0.014	1.502GlnAsp: 1.502 ± 0.037	1.538GlnGlu: 1.538 ± 0.038	1.068GlnPhe: 1.068 ± 0.03	2.316GlnGly: 2.316 ± 0.045	0.626GlnHis: 0.626 ± 0.027	1.818GlnIle: 1.818 ± 0.04	1.325GlnLys: 1.325 ± 0.034	2.769GlnLeu: 2.769 ± 0.052	0.942GlnMet: 0.942 ± 0.029	0.886GlnAsn: 0.886 ± 0.028	1.772GlnPro: 1.772 ± 0.041	1.189GlnGln: 1.189 ± 0.037	2.367GlnArg: 2.367 ± 0.045	1.797GlnSer: 1.797 ± 0.042	1.686GlnThr: 1.686 ± 0.036	2.145GlnVal: 2.145 ± 0.044	0.407GlnTrp: 0.407 ± 0.018	0.614GlnTyr: 0.614 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	7.184ArgAla: 7.184 ± 0.082	0.45ArgCys: 0.45 ± 0.021	3.857ArgAsp: 3.857 ± 0.056	3.974ArgGlu: 3.974 ± 0.074	2.984ArgPhe: 2.984 ± 0.053	4.507ArgGly: 4.507 ± 0.068	1.667ArgHis: 1.667 ± 0.037	3.991ArgIle: 3.991 ± 0.055	2.79ArgLys: 2.79 ± 0.053	7.457ArgLeu: 7.457 ± 0.095	1.833ArgMet: 1.833 ± 0.035	1.966ArgAsn: 1.966 ± 0.045	3.24ArgPro: 3.24 ± 0.062	2.617ArgGln: 2.617 ± 0.053	5.149ArgArg: 5.149 ± 0.084	3.748ArgSer: 3.748 ± 0.062	3.302ArgThr: 3.302 ± 0.056	4.346ArgVal: 4.346 ± 0.066	0.851ArgTrp: 0.851 ± 0.031	1.661ArgTyr: 1.661 ± 0.038	0.002ArgXaa: 0.002 ± 0.001
Ser	6.362SerAla: 6.362 ± 0.078	0.39SerCys: 0.39 ± 0.02	3.11SerAsp: 3.11 ± 0.055	2.969SerGlu: 2.969 ± 0.048	2.391SerPhe: 2.391 ± 0.045	5.892SerGly: 5.892 ± 0.084	1.14SerHis: 1.14 ± 0.033	3.046SerIle: 3.046 ± 0.055	1.861SerLys: 1.861 ± 0.039	5.463SerLeu: 5.463 ± 0.083	1.434SerMet: 1.434 ± 0.032	1.492SerAsn: 1.492 ± 0.034	2.642SerPro: 2.642 ± 0.045	1.7SerGln: 1.7 ± 0.041	3.65SerArg: 3.65 ± 0.06	3.003SerSer: 3.003 ± 0.053	2.775SerThr: 2.775 ± 0.044	4.196SerVal: 4.196 ± 0.055	0.755SerTrp: 0.755 ± 0.028	1.283SerTyr: 1.283 ± 0.034	0.003SerXaa: 0.003 ± 0.002
Thr	6.181ThrAla: 6.181 ± 0.075	0.418ThrCys: 0.418 ± 0.02	2.829ThrAsp: 2.829 ± 0.052	2.663ThrGlu: 2.663 ± 0.047	2.068ThrPhe: 2.068 ± 0.039	5.163ThrGly: 5.163 ± 0.07	0.942ThrHis: 0.942 ± 0.028	3.01ThrIle: 3.01 ± 0.055	1.609ThrLys: 1.609 ± 0.037	5.443ThrLeu: 5.443 ± 0.074	1.312ThrMet: 1.312 ± 0.032	1.371ThrAsn: 1.371 ± 0.041	2.892ThrPro: 2.892 ± 0.05	1.385ThrGln: 1.385 ± 0.037	3.182ThrArg: 3.182 ± 0.05	2.785ThrSer: 2.785 ± 0.052	2.892ThrThr: 2.892 ± 0.052	4.547ThrVal: 4.547 ± 0.074	0.606ThrTrp: 0.606 ± 0.02	1.234ThrTyr: 1.234 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	9.352ValAla: 9.352 ± 0.081	0.606ValCys: 0.606 ± 0.024	4.267ValAsp: 4.267 ± 0.063	4.871ValGlu: 4.871 ± 0.071	2.925ValPhe: 2.925 ± 0.052	5.724ValGly: 5.724 ± 0.076	1.42ValHis: 1.42 ± 0.031	3.994ValIle: 3.994 ± 0.06	2.732ValLys: 2.732 ± 0.047	7.232ValLeu: 7.232 ± 0.079	1.853ValMet: 1.853 ± 0.037	1.987ValAsn: 1.987 ± 0.045	3.667ValPro: 3.667 ± 0.058	2.013ValGln: 2.013 ± 0.043	4.758ValArg: 4.758 ± 0.071	4.697ValSer: 4.697 ± 0.067	4.438ValThr: 4.438 ± 0.069	6.102ValVal: 6.102 ± 0.069	0.875ValTrp: 0.875 ± 0.027	1.483ValTyr: 1.483 ± 0.039	0.002ValXaa: 0.002 ± 0.001
Trp	1.22TrpAla: 1.22 ± 0.037	0.132TrpCys: 0.132 ± 0.01	0.594TrpAsp: 0.594 ± 0.023	0.546TrpGlu: 0.546 ± 0.022	0.534TrpPhe: 0.534 ± 0.024	0.81TrpGly: 0.81 ± 0.028	0.323TrpHis: 0.323 ± 0.016	0.623TrpIle: 0.623 ± 0.023	0.539TrpLys: 0.539 ± 0.021	1.578TrpLeu: 1.578 ± 0.042	0.366TrpMet: 0.366 ± 0.018	0.467TrpAsn: 0.467 ± 0.02	0.654TrpPro: 0.654 ± 0.023	0.59TrpGln: 0.59 ± 0.022	1.076TrpArg: 1.076 ± 0.03	0.815TrpSer: 0.815 ± 0.026	0.704TrpThr: 0.704 ± 0.024	0.787TrpVal: 0.787 ± 0.026	0.231TrpTrp: 0.231 ± 0.013	0.283TrpTyr: 0.283 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.484TyrAla: 2.484 ± 0.042	0.237TyrCys: 0.237 ± 0.014	1.513TyrAsp: 1.513 ± 0.035	1.223TyrGlu: 1.223 ± 0.039	0.911TyrPhe: 0.911 ± 0.026	2.121TyrGly: 2.121 ± 0.047	0.465TyrHis: 0.465 ± 0.02	0.906TyrIle: 0.906 ± 0.025	0.753TyrLys: 0.753 ± 0.024	2.236TyrLeu: 2.236 ± 0.043	0.511TyrMet: 0.511 ± 0.023	0.67TyrAsn: 0.67 ± 0.022	1.08TyrPro: 1.08 ± 0.03	0.727TyrGln: 0.727 ± 0.025	1.756TyrArg: 1.756 ± 0.038	1.21TyrSer: 1.21 ± 0.034	1.119TyrThr: 1.119 ± 0.033	1.675TyrVal: 1.675 ± 0.035	0.356TyrTrp: 0.356 ± 0.015	0.602TyrTyr: 0.602 ± 0.02	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.003XaaGly: 0.003 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 3925 proteins (1190505 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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