Legionella israelensis
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2821 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W0VHG3|A0A0W0VHG3_9GAMM DNA-binding protein fis OS=Legionella israelensis OX=454 GN=fis_3 PE=3 SV=1
MM1 pKa = 7.09 TAVDD5 pKa = 4.46 NIQNKK10 pKa = 7.73 DD11 pKa = 3.25 THH13 pKa = 7.34 NEE15 pKa = 3.28 ADD17 pKa = 3.19 IYY19 pKa = 10.84 FSVSAADD26 pKa = 4.04 KK27 pKa = 9.69 VASLIEE33 pKa = 4.14 EE34 pKa = 4.3 EE35 pKa = 4.76 EE36 pKa = 4.13 NPKK39 pKa = 10.88 LNLRR43 pKa = 11.84 VYY45 pKa = 9.02 ITGGGCSGFQYY56 pKa = 11.01 GFTFDD61 pKa = 4.7 EE62 pKa = 5.2 NIADD66 pKa = 4.55 DD67 pKa = 4.04 DD68 pKa = 4.41 TVIEE72 pKa = 4.22 QACSNGEE79 pKa = 4.06 SSVKK83 pKa = 10.57 LLVDD87 pKa = 3.58 SMSYY91 pKa = 10.53 QYY93 pKa = 10.74 LQSAEE98 pKa = 3.51 IDD100 pKa = 4.0 YY101 pKa = 10.49 IQSIQGEE108 pKa = 4.36 QFVIRR113 pKa = 11.84 NPNAKK118 pKa = 6.55 TTCGCGSSFSIDD130 pKa = 3.59 EE131 pKa = 5.09 DD132 pKa = 4.25 DD133 pKa = 5.27 DD134 pKa = 4.13 SS135 pKa = 5.25
Molecular weight: 14.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.554
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.884
Patrickios 0.655
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A0W0V2C0|A0A0W0V2C0_9GAMM Outer membrane protein OS=Legionella israelensis OX=454 GN=Lisr_2782 PE=4 SV=1
MM1 pKa = 7.81 AEE3 pKa = 3.82 KK4 pKa = 9.86 QQYY7 pKa = 9.41 YY8 pKa = 8.16 GTGRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.95 NSSARR19 pKa = 11.84 VFLRR23 pKa = 11.84 PGKK26 pKa = 10.95 GEE28 pKa = 3.69 IKK30 pKa = 10.32 INNRR34 pKa = 11.84 LLEE37 pKa = 4.73 DD38 pKa = 3.47 YY39 pKa = 10.57 FGRR42 pKa = 11.84 QTSCMLVRR50 pKa = 11.84 QPLEE54 pKa = 3.94 TVDD57 pKa = 5.6 AIDD60 pKa = 4.92 KK61 pKa = 10.16 FDD63 pKa = 3.61 IYY65 pKa = 11.21 ATVTGGGISGQAGAVRR81 pKa = 11.84 LGIARR86 pKa = 11.84 ALVQYY91 pKa = 10.92 DD92 pKa = 3.77 EE93 pKa = 4.91 VGLSEE98 pKa = 4.72 GSEE101 pKa = 4.06 PDD103 pKa = 3.13 PSSFRR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 LRR112 pKa = 11.84 ARR114 pKa = 11.84 GLLTRR119 pKa = 11.84 DD120 pKa = 3.3 PRR122 pKa = 11.84 RR123 pKa = 11.84 VEE125 pKa = 3.87 RR126 pKa = 11.84 KK127 pKa = 9.52 KK128 pKa = 11.17 VGLHH132 pKa = 5.33 KK133 pKa = 10.57 ARR135 pKa = 11.84 RR136 pKa = 11.84 ATQYY140 pKa = 10.97 SKK142 pKa = 11.25 RR143 pKa = 3.79
Molecular weight: 16.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.809
IPC_protein 10.745
Toseland 10.789
ProMoST 10.76
Dawson 10.877
Bjellqvist 10.628
Wikipedia 11.125
Rodwell 11.023
Grimsley 10.935
Solomon 11.038
Lehninger 10.994
Nozaki 10.76
DTASelect 10.628
Thurlkill 10.789
EMBOSS 11.199
Sillero 10.818
Patrickios 10.745
IPC_peptide 11.038
IPC2_peptide 9.619
IPC2.peptide.svr19 8.582
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2821
0
2821
878026
50
3739
311.2
35.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.433 ± 0.043
1.195 ± 0.016
4.874 ± 0.039
6.302 ± 0.05
4.604 ± 0.038
5.887 ± 0.042
2.672 ± 0.026
7.447 ± 0.04
6.692 ± 0.047
10.89 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.49 ± 0.02
4.585 ± 0.037
3.938 ± 0.027
4.568 ± 0.038
4.499 ± 0.038
6.579 ± 0.03
4.839 ± 0.03
5.751 ± 0.041
1.195 ± 0.017
3.547 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here