Torque teno virus 7
Average proteome isoelectric point is 7.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9IFV1|Q9IFV1_9VIRU PORF2 OS=Torque teno virus 7 OX=687346 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.54 FFGRR5 pKa = 11.84 PWRR8 pKa = 11.84 KK9 pKa = 9.07 KK10 pKa = 9.98 RR11 pKa = 11.84 KK12 pKa = 9.5 VPLPPLQTEE21 pKa = 4.44 PLQPPIHH28 pKa = 6.18 MSLWRR33 pKa = 11.84 PPVYY37 pKa = 10.19 NVPGQEE43 pKa = 3.77 RR44 pKa = 11.84 QFYY47 pKa = 7.46 EE48 pKa = 4.47 ACLHH52 pKa = 6.2 AHH54 pKa = 7.24 DD55 pKa = 5.35 AFCGCGDD62 pKa = 4.19 FVAHH66 pKa = 6.64 INSVAARR73 pKa = 11.84 VGRR76 pKa = 11.84 PSDD79 pKa = 3.67 NQPPRR84 pKa = 11.84 PQRR87 pKa = 11.84 PPAIRR92 pKa = 11.84 SMRR95 pKa = 11.84 ALPAPPSSGTSTDD108 pKa = 3.21 NQGAQWPGDD117 pKa = 3.57 GGGEE121 pKa = 4.14 GAEE124 pKa = 4.7 GGPDD128 pKa = 3.49 DD129 pKa = 5.57 AGPPGDD135 pKa = 4.85 GDD137 pKa = 4.07 LAPEE141 pKa = 4.23 DD142 pKa = 3.87 VEE144 pKa = 4.54 EE145 pKa = 4.12 LLEE148 pKa = 4.17 LVEE151 pKa = 4.16 EE152 pKa = 4.74 AEE154 pKa = 4.26
Molecular weight: 16.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.892
IPC2_protein 4.914
IPC_protein 4.8
Toseland 4.698
ProMoST 4.838
Dawson 4.774
Bjellqvist 4.952
Wikipedia 4.647
Rodwell 4.685
Grimsley 4.622
Solomon 4.774
Lehninger 4.724
Nozaki 4.889
DTASelect 5.041
Thurlkill 4.698
EMBOSS 4.673
Sillero 4.952
Patrickios 4.088
IPC_peptide 4.774
IPC2_peptide 4.952
IPC2.peptide.svr19 4.869
Protein with the highest isoelectric point:
>tr|Q9IFV1|Q9IFV1_9VIRU PORF2 OS=Torque teno virus 7 OX=687346 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.56 AWRR4 pKa = 11.84 WWWRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 WPRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 TTWRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 PRR26 pKa = 11.84 PRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 TARR35 pKa = 11.84 TRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 GRR41 pKa = 11.84 VRR43 pKa = 11.84 RR44 pKa = 11.84 WRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 GRR50 pKa = 11.84 GWRR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 TYY57 pKa = 8.92 IRR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 KK65 pKa = 9.76 KK66 pKa = 10.36 KK67 pKa = 10.52 INIQQWNPATVKK79 pKa = 9.86 KK80 pKa = 10.51 CVVTGYY86 pKa = 10.71 VPLLICGTGTTGTTYY101 pKa = 11.09 KK102 pKa = 10.83 NYY104 pKa = 10.14 GSHH107 pKa = 6.46 INDD110 pKa = 3.24 YY111 pKa = 10.25 TKK113 pKa = 10.61 FDD115 pKa = 3.9 PFGGGFSTLMFNLRR129 pKa = 11.84 ILFDD133 pKa = 3.67 EE134 pKa = 4.58 YY135 pKa = 11.25 KK136 pKa = 10.24 KK137 pKa = 10.61 QRR139 pKa = 11.84 CRR141 pKa = 11.84 WSRR144 pKa = 11.84 SNDD147 pKa = 3.03 EE148 pKa = 4.55 LEE150 pKa = 3.92 LVRR153 pKa = 11.84 YY154 pKa = 8.52 LGCSFTLYY162 pKa = 10.44 RR163 pKa = 11.84 EE164 pKa = 4.12 QNVDD168 pKa = 4.71 FLFKK172 pKa = 10.74 YY173 pKa = 10.3 NRR175 pKa = 11.84 RR176 pKa = 11.84 QPFSDD181 pKa = 3.55 SQLTGPSLHH190 pKa = 7.1 PGIIMKK196 pKa = 9.41 QKK198 pKa = 10.03 RR199 pKa = 11.84 KK200 pKa = 10.52 VIVPSYY206 pKa = 8.21 KK207 pKa = 9.65 TKK209 pKa = 10.5 PKK211 pKa = 9.85 GRR213 pKa = 11.84 PVKK216 pKa = 10.3 RR217 pKa = 11.84 IRR219 pKa = 11.84 IKK221 pKa = 10.79 PPTLFTDD228 pKa = 3.11 RR229 pKa = 11.84 WYY231 pKa = 10.5 FQKK234 pKa = 10.85 DD235 pKa = 3.44 FSNFPLVTISASAASLRR252 pKa = 11.84 FPFCSPQTGNICIYY266 pKa = 10.28 FQILDD271 pKa = 4.06 PYY273 pKa = 8.59 WYY275 pKa = 9.53 NRR277 pKa = 11.84 RR278 pKa = 11.84 MSITPDD284 pKa = 3.4 LLKK287 pKa = 11.0 SNYY290 pKa = 9.46 EE291 pKa = 4.07 SFFSVLNTKK300 pKa = 10.31 FSSSPLYY307 pKa = 10.18 PNVATTVPSGPIGTVFNTFKK327 pKa = 9.8 TQEE330 pKa = 4.26 HH331 pKa = 5.57 VVDD334 pKa = 4.49 PRR336 pKa = 11.84 WDD338 pKa = 3.66 TVKK341 pKa = 10.59 KK342 pKa = 10.56 QSTGSQWSNTFVDD355 pKa = 4.17 SHH357 pKa = 6.39 WGDD360 pKa = 3.8 HH361 pKa = 6.11 IYY363 pKa = 11.1 EE364 pKa = 4.26 NTSSKK369 pKa = 11.02 SILTAMKK376 pKa = 10.67 DD377 pKa = 3.68 NATKK381 pKa = 10.0 MYY383 pKa = 10.5 DD384 pKa = 2.91 RR385 pKa = 11.84 RR386 pKa = 11.84 KK387 pKa = 9.61 RR388 pKa = 11.84 DD389 pKa = 3.03 TYY391 pKa = 11.2 LGSKK395 pKa = 8.57 YY396 pKa = 10.65 LNYY399 pKa = 9.37 RR400 pKa = 11.84 TGLYY404 pKa = 10.54 SSIFLANEE412 pKa = 3.7 RR413 pKa = 11.84 TSPDD417 pKa = 3.27 FPGLYY422 pKa = 9.76 QEE424 pKa = 5.13 VVYY427 pKa = 11.1 NPLVDD432 pKa = 3.69 EE433 pKa = 4.73 GEE435 pKa = 4.39 GNIVWIDD442 pKa = 3.25 WCSKK446 pKa = 10.98 DD447 pKa = 3.37 DD448 pKa = 3.55 TTFRR452 pKa = 11.84 DD453 pKa = 3.58 LPQRR457 pKa = 11.84 YY458 pKa = 8.36 AVKK461 pKa = 10.47 DD462 pKa = 3.45 IPLWAAFMGYY472 pKa = 9.14 RR473 pKa = 11.84 DD474 pKa = 4.3 YY475 pKa = 10.69 VTKK478 pKa = 10.65 ALHH481 pKa = 7.12 DD482 pKa = 4.66 PGLSKK487 pKa = 10.41 EE488 pKa = 4.09 VRR490 pKa = 11.84 VTIICPYY497 pKa = 9.29 TKK499 pKa = 10.04 PKK501 pKa = 10.46 LYY503 pKa = 10.98 NPDD506 pKa = 3.6 STDD509 pKa = 3.06 EE510 pKa = 4.83 GYY512 pKa = 11.08 VPYY515 pKa = 10.19 DD516 pKa = 3.44 YY517 pKa = 11.68 NFGKK521 pKa = 10.9 GKK523 pKa = 9.31 MPDD526 pKa = 3.25 GNGYY530 pKa = 9.63 IPIAYY535 pKa = 9.2 RR536 pKa = 11.84 FQWYY540 pKa = 8.8 PCMFHH545 pKa = 5.9 QQNFMNDD552 pKa = 3.06 IVQSGPFAYY561 pKa = 10.04 HH562 pKa = 6.84 GEE564 pKa = 4.33 EE565 pKa = 4.3 KK566 pKa = 10.69 SCTLTAKK573 pKa = 10.28 YY574 pKa = 10.15 RR575 pKa = 11.84 FRR577 pKa = 11.84 FLFGGNPISQQVIKK591 pKa = 10.59 DD592 pKa = 3.59 PSKK595 pKa = 11.07 QPDD598 pKa = 3.64 FQIPGARR605 pKa = 11.84 DD606 pKa = 3.25 LFSTVQVTNPKK617 pKa = 10.38 LIDD620 pKa = 3.23 EE621 pKa = 5.24 GYY623 pKa = 10.55 FFNAWDD629 pKa = 3.28 IRR631 pKa = 11.84 RR632 pKa = 11.84 GLFGFSAIKK641 pKa = 10.39 RR642 pKa = 11.84 MQNQQIPTKK651 pKa = 10.37 YY652 pKa = 8.68 FTGPPKK658 pKa = 10.32 RR659 pKa = 11.84 PRR661 pKa = 11.84 FEE663 pKa = 4.0 VPAIANAGSDD673 pKa = 3.28 LHH675 pKa = 5.49 PTKK678 pKa = 9.76 WHH680 pKa = 6.36 PWNEE684 pKa = 4.0 TSEE687 pKa = 4.27 EE688 pKa = 4.06 EE689 pKa = 4.0 EE690 pKa = 4.2 DD691 pKa = 3.69 QKK693 pKa = 11.57 EE694 pKa = 4.22 DD695 pKa = 3.8 SPSPQTKK702 pKa = 10.45 APLQLILKK710 pKa = 8.3 QQLRR714 pKa = 11.84 EE715 pKa = 3.8 QRR717 pKa = 11.84 EE718 pKa = 4.18 LKK720 pKa = 10.46 RR721 pKa = 11.84 RR722 pKa = 11.84 IQFLVKK728 pKa = 10.31 QLVKK732 pKa = 9.55 TQYY735 pKa = 9.9 HH736 pKa = 5.52 LHH738 pKa = 6.38 APIIPSRR745 pKa = 11.84 TSSLTVPRR753 pKa = 11.84 DD754 pKa = 3.42 DD755 pKa = 4.24 QKK757 pKa = 11.86 NIFQQRR763 pKa = 11.84 LEE765 pKa = 4.02 MM766 pKa = 5.13
Molecular weight: 90.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.294
IPC2_protein 9.663
IPC_protein 10.101
Toseland 10.277
ProMoST 9.999
Dawson 10.467
Bjellqvist 10.175
Wikipedia 10.657
Rodwell 10.804
Grimsley 10.54
Solomon 10.511
Lehninger 10.467
Nozaki 10.277
DTASelect 10.16
Thurlkill 10.321
EMBOSS 10.687
Sillero 10.394
Patrickios 10.365
IPC_peptide 10.511
IPC2_peptide 9.019
IPC2.peptide.svr19 8.643
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
974
54
766
324.7
38.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.107 ± 1.853
1.54 ± 0.616
5.544 ± 0.36
4.107 ± 1.377
5.441 ± 0.879
6.16 ± 1.947
1.643 ± 0.498
4.62 ± 1.362
6.468 ± 2.136
6.263 ± 0.186
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.745 ± 0.532
4.004 ± 0.852
8.83 ± 2.747
5.133 ± 0.117
9.651 ± 1.983
6.263 ± 0.904
6.366 ± 1.637
4.62 ± 0.708
2.875 ± 0.712
4.62 ± 1.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here