Apibacter mensalis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Apibacter

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2083 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A110BK62|A0A110BK62_9FLAO Intein N-terminal splicing region OS=Apibacter mensalis OX=1586267 GN=Ga0061079_104118 PE=4 SV=1
MM1 pKa = 7.67TIYY4 pKa = 10.28EE5 pKa = 4.29LSIDD9 pKa = 3.91GDD11 pKa = 3.89NATSLDD17 pKa = 3.95FPIQDD22 pKa = 3.5EE23 pKa = 4.45QEE25 pKa = 4.19QFLLLAEE32 pKa = 4.29EE33 pKa = 5.2KK34 pKa = 9.34ITNNLSVKK42 pKa = 10.25AQWQEE47 pKa = 3.52FLVLKK52 pKa = 10.44KK53 pKa = 10.39EE54 pKa = 4.3PKK56 pKa = 9.54IDD58 pKa = 3.55MDD60 pKa = 4.29FNDD63 pKa = 3.79INDD66 pKa = 3.39IGVYY70 pKa = 9.96IISPRR75 pKa = 11.84AYY77 pKa = 9.76EE78 pKa = 3.97LLHH81 pKa = 6.49PLFDD85 pKa = 5.25DD86 pKa = 3.86SVEE89 pKa = 4.06VLPLKK94 pKa = 10.71SDD96 pKa = 3.53DD97 pKa = 3.48GTYY100 pKa = 10.82FLLNIIQTTDD110 pKa = 3.36CLDD113 pKa = 3.85KK114 pKa = 11.2EE115 pKa = 4.42NSVYY119 pKa = 10.07TALPGGQILEE129 pKa = 4.24YY130 pKa = 9.79EE131 pKa = 4.48NLSIDD136 pKa = 3.9PQKK139 pKa = 9.67ITCPLFRR146 pKa = 11.84IPEE149 pKa = 4.03LPYY152 pKa = 10.18TVLITDD158 pKa = 3.99QIQDD162 pKa = 2.83IFYY165 pKa = 9.46SHH167 pKa = 5.95QLKK170 pKa = 10.45GLHH173 pKa = 4.93FTEE176 pKa = 4.76NEE178 pKa = 4.06FVTSDD183 pKa = 3.25FF184 pKa = 4.09

Molecular weight: 21.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X3APA2|A0A0X3APA2_9FLAO Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Apibacter mensalis OX=1586267 GN=dut PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84IVNNFEE22 pKa = 4.71EE23 pKa = 4.61ITTSKK28 pKa = 10.54PEE30 pKa = 3.67KK31 pKa = 10.07SLLRR35 pKa = 11.84GISKK39 pKa = 10.57SGGRR43 pKa = 11.84NNSGKK48 pKa = 8.25MTMRR52 pKa = 11.84YY53 pKa = 7.81TGGGHH58 pKa = 6.19KK59 pKa = 9.81KK60 pKa = 9.9RR61 pKa = 11.84YY62 pKa = 9.52RR63 pKa = 11.84EE64 pKa = 3.64IDD66 pKa = 3.38FKK68 pKa = 11.17RR69 pKa = 11.84NKK71 pKa = 9.89FGIPATVKK79 pKa = 10.48SVEE82 pKa = 3.91YY83 pKa = 10.55DD84 pKa = 3.39PNRR87 pKa = 11.84TAFIALLVYY96 pKa = 10.85ADD98 pKa = 3.54GEE100 pKa = 4.0KK101 pKa = 10.23RR102 pKa = 11.84YY103 pKa = 9.71IVSPHH108 pKa = 6.44GIKK111 pKa = 10.33VGQKK115 pKa = 8.2VVSGEE120 pKa = 4.14SVAPEE125 pKa = 3.68IGNAMKK131 pKa = 10.68LKK133 pKa = 10.01NIPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAKK153 pKa = 8.51MARR156 pKa = 11.84SAGTSAQLIARR167 pKa = 11.84DD168 pKa = 3.38GKK170 pKa = 10.41YY171 pKa = 10.39AVLKK175 pKa = 9.42MPSGEE180 pKa = 3.74TRR182 pKa = 11.84MILVEE187 pKa = 4.22CMATIGSVSNSDD199 pKa = 2.99HH200 pKa = 5.87QLIVSGKK207 pKa = 8.58AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.0GRR238 pKa = 11.84STGGHH243 pKa = 5.23PRR245 pKa = 11.84SRR247 pKa = 11.84KK248 pKa = 9.07GMPAKK253 pKa = 10.34GYY255 pKa = 7.47KK256 pKa = 8.79TRR258 pKa = 11.84SKK260 pKa = 10.53KK261 pKa = 10.32KK262 pKa = 8.79EE263 pKa = 3.86SNRR266 pKa = 11.84YY267 pKa = 8.71ILQKK271 pKa = 10.66RR272 pKa = 11.84KK273 pKa = 9.96

Molecular weight: 30.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2083

0

2083

668283

39

2439

320.8

36.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.24 ± 0.053

0.835 ± 0.017

5.112 ± 0.036

6.517 ± 0.054

5.134 ± 0.045

6.101 ± 0.056

1.742 ± 0.019

9.389 ± 0.061

8.924 ± 0.05

9.427 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.02

6.518 ± 0.055

3.304 ± 0.03

3.345 ± 0.026

3.248 ± 0.029

6.754 ± 0.043

5.243 ± 0.039

5.469 ± 0.038

0.985 ± 0.017

4.607 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski