Andreprevotia lacus DSM 23236
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4303 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W1Y0Q2|A0A1W1Y0Q2_9NEIS Multidrug efflux pump OS=Andreprevotia lacus DSM 23236 OX=1121001 GN=SAMN02745857_04055 PE=4 SV=1
MM1 pKa = 7.47 ATPSQQATIVQLNGQAFIRR20 pKa = 11.84 DD21 pKa = 3.92 ATGKK25 pKa = 7.27 MTPLHH30 pKa = 6.46 SGEE33 pKa = 4.78 TLNQDD38 pKa = 3.07 QVLVTSPDD46 pKa = 3.48 GEE48 pKa = 4.5 VVVLLPNGDD57 pKa = 3.83 TQTVGPDD64 pKa = 2.87 RR65 pKa = 11.84 SVLLDD70 pKa = 3.57 AQFMGAAPDD79 pKa = 3.63 TTEE82 pKa = 3.98 AAVAPSSVQGGTAQIDD98 pKa = 3.57 QIVAQGGDD106 pKa = 3.28 LSTDD110 pKa = 3.94 LEE112 pKa = 4.22 ATAAGLTGGGDD123 pKa = 3.61 AGNGTHH129 pKa = 5.71 TFIRR133 pKa = 11.84 VGRR136 pKa = 11.84 ISEE139 pKa = 4.37 EE140 pKa = 3.94 AGSTAFGTPGTPIGGAGAAEE160 pKa = 3.96 QNGQNHH166 pKa = 6.39 APVAVDD172 pKa = 4.31 DD173 pKa = 4.67 NSTGAANDD181 pKa = 3.99 GLTSPEE187 pKa = 4.24 DD188 pKa = 3.78 TPLTISPLLLLSNDD202 pKa = 3.8 GDD204 pKa = 4.27 PDD206 pKa = 4.4 GDD208 pKa = 3.97 PLTIVSVQDD217 pKa = 3.69 PVHH220 pKa = 5.86 GTLSIDD226 pKa = 3.34 ANNNIVFTPDD236 pKa = 3.08 PNYY239 pKa = 10.8 NGPASFSYY247 pKa = 10.47 TIDD250 pKa = 3.62 DD251 pKa = 4.05 GRR253 pKa = 11.84 GGTATATVNLNITPVNDD270 pKa = 3.72 PATVSNDD277 pKa = 2.8 AGTVKK282 pKa = 10.47 EE283 pKa = 4.3 DD284 pKa = 3.61 TPTQTTVGGTLTITDD299 pKa = 3.44 VDD301 pKa = 3.43 TGEE304 pKa = 4.12 QHH306 pKa = 6.82 VVAQNVSNPYY316 pKa = 8.87 GTFTVDD322 pKa = 5.17 ADD324 pKa = 4.4 GNWSFTIDD332 pKa = 3.46 NTSPTVQALAEE343 pKa = 4.38 GQTQVLTFDD352 pKa = 3.67 VTSVDD357 pKa = 3.41 GTGTGVVTITVVGTNDD373 pKa = 3.14 NAVITAHH380 pKa = 6.98 LPGDD384 pKa = 3.63 DD385 pKa = 3.67 AGTVKK390 pKa = 10.61 EE391 pKa = 4.2 DD392 pKa = 3.86 TILATGGKK400 pKa = 9.7 LDD402 pKa = 4.3 VADD405 pKa = 5.15 PDD407 pKa = 3.94 TGEE410 pKa = 4.26 SAVIAQTDD418 pKa = 3.53 TTGTYY423 pKa = 10.83 GSFSILADD431 pKa = 3.74 GTWTYY436 pKa = 9.98 TLNNASPVVQALNEE450 pKa = 4.4 GEE452 pKa = 4.18 SRR454 pKa = 11.84 TEE456 pKa = 3.78 VFQVQSQDD464 pKa = 2.92 GSDD467 pKa = 3.21 THH469 pKa = 6.62 FVTVTVLGTNDD480 pKa = 3.21 NAVITAHH487 pKa = 6.98 LPGDD491 pKa = 3.63 DD492 pKa = 3.67 AGTVKK497 pKa = 10.61 EE498 pKa = 4.24 DD499 pKa = 4.14 TILSTGGKK507 pKa = 9.71 LDD509 pKa = 3.81 VADD512 pKa = 4.76 PDD514 pKa = 3.94 AGQSAVVAQSNTTGTYY530 pKa = 10.27 GSFSIAADD538 pKa = 3.93 GTWTYY543 pKa = 11.53 SLNNGSPIVQALNEE557 pKa = 4.42 GEE559 pKa = 4.37 SRR561 pKa = 11.84 TEE563 pKa = 3.84 TFQVQSLDD571 pKa = 3.4 GTDD574 pKa = 3.14 THH576 pKa = 6.4 TVTVTILGTNDD587 pKa = 2.93 AALIGGNDD595 pKa = 3.42 SGTVKK600 pKa = 10.68 EE601 pKa = 4.5 DD602 pKa = 3.31 TTLVTGGILTVSDD615 pKa = 3.57 VDD617 pKa = 3.74 TGQSSLVAEE626 pKa = 4.72 TVTGTYY632 pKa = 8.95 GTFSVDD638 pKa = 4.21 ANGNWSYY645 pKa = 11.82 VLNNSSPIVQALNDD659 pKa = 3.43 GDD661 pKa = 4.05 TRR663 pKa = 11.84 VEE665 pKa = 4.16 TFAVQSVDD673 pKa = 3.3 GTTHH677 pKa = 5.92 TVTVNVLGTNDD688 pKa = 4.21 GEE690 pKa = 4.34 QLPPPRR696 pKa = 11.84 ISVSLDD702 pKa = 3.24 SEE704 pKa = 4.36 GDD706 pKa = 3.69 TVFEE710 pKa = 3.85 GNYY713 pKa = 10.63 AVFNVHH719 pKa = 6.77 LSYY722 pKa = 10.97 PSTTDD727 pKa = 3.01 VTFTPSQVSGTATVGTDD744 pKa = 3.05 TSDD747 pKa = 3.18 TLEE750 pKa = 4.13 YY751 pKa = 9.98 WNGSAWVAVSGGITIPAGQTDD772 pKa = 3.84 VQVRR776 pKa = 11.84 VATVADD782 pKa = 5.15 GIYY785 pKa = 10.2 EE786 pKa = 4.17 GNEE789 pKa = 3.81 NFSLHH794 pKa = 6.36 VDD796 pKa = 3.56 VTSGSTSNTSADD808 pKa = 3.39 ATAVIIDD815 pKa = 3.71 GDD817 pKa = 4.0 KK818 pKa = 10.62 PPEE821 pKa = 3.87 VSISVSGEE829 pKa = 3.08 GSMVHH834 pKa = 6.17 EE835 pKa = 4.48 GDD837 pKa = 3.58 YY838 pKa = 11.46 AVFNVHH844 pKa = 6.73 LSGSSSTDD852 pKa = 3.22 VVFTPSLVSGTATVGTDD869 pKa = 2.5 TGTALEE875 pKa = 4.19 YY876 pKa = 11.0 SLNGTDD882 pKa = 3.08 WFAVGTGVTIPAGQTDD898 pKa = 3.84 VQVRR902 pKa = 11.84 VATVADD908 pKa = 5.15 GIYY911 pKa = 10.2 EE912 pKa = 4.17 GNEE915 pKa = 3.81 NFSLHH920 pKa = 6.47 VDD922 pKa = 3.54 VTSSNTSNSSADD934 pKa = 3.29 ATATIVDD941 pKa = 4.34 ADD943 pKa = 4.28 SPPQVTVSVSGEE955 pKa = 3.34 GSMVHH960 pKa = 6.17 EE961 pKa = 4.48 GDD963 pKa = 3.58 YY964 pKa = 11.46 AVFNVHH970 pKa = 6.73 LSGSSSTDD978 pKa = 3.22 VVFTPSLVSGTATVGTDD995 pKa = 2.5 TGTALEE1001 pKa = 4.3 YY1002 pKa = 10.23 WNGSAWVAISGGVTIPAGQTDD1023 pKa = 3.84 VQVRR1027 pKa = 11.84 VATVADD1033 pKa = 5.15 GIYY1036 pKa = 10.2 EE1037 pKa = 4.17 GNEE1040 pKa = 3.81 NFSLHH1045 pKa = 6.47 VDD1047 pKa = 3.54 VTSSNTSNTSADD1059 pKa = 3.31 ATATIIDD1066 pKa = 4.17 ADD1068 pKa = 4.1 SPPVVSVSVSSEE1080 pKa = 3.22 GSMVPEE1086 pKa = 4.12 GAYY1089 pKa = 10.47 AVFNVHH1095 pKa = 6.84 LSGPSSGAISFTPSLHH1111 pKa = 7.25 DD1112 pKa = 3.2 GTAIIGTDD1120 pKa = 2.95 TSTTLEE1126 pKa = 4.2 YY1127 pKa = 10.42 WNGSAWTVVSGDD1139 pKa = 2.95 ITLAAGQTDD1148 pKa = 3.77 VQVRR1152 pKa = 11.84 VATIDD1157 pKa = 3.43 DD1158 pKa = 4.24 SIYY1161 pKa = 10.71 EE1162 pKa = 4.16 GNEE1165 pKa = 3.65 NFSLQVHH1172 pKa = 5.22 VTSGSTSNTDD1182 pKa = 3.0 ASATAIIVDD1191 pKa = 3.96 NDD1193 pKa = 3.72 AQPVGHH1199 pKa = 7.59 DD1200 pKa = 3.8 DD1201 pKa = 4.16 GYY1203 pKa = 9.47 TVQEE1207 pKa = 4.07 ASTNALASVLGNDD1220 pKa = 4.02 TPSGLHH1226 pKa = 4.49 VAKK1229 pKa = 10.21 FATDD1233 pKa = 3.6 GAGGGAVSADD1243 pKa = 3.23 GSHH1246 pKa = 7.48 TITTSLGGTVTMHH1259 pKa = 7.51 ADD1261 pKa = 3.22 GTFTYY1266 pKa = 8.65 TIPDD1270 pKa = 3.54 LHH1272 pKa = 6.46 HH1273 pKa = 6.98 TGTAVDD1279 pKa = 3.52 SFYY1282 pKa = 11.59 YY1283 pKa = 10.12 QATDD1287 pKa = 3.61 GNANTAWTKK1296 pKa = 10.47 VSVNVTDD1303 pKa = 4.07 TGPTAHH1309 pKa = 7.81 DD1310 pKa = 3.83 DD1311 pKa = 3.98 TVSLNTVSTDD1321 pKa = 3.15 TVNLVLVVDD1330 pKa = 4.33 SSGSIGTTNLNTIKK1344 pKa = 10.63 AALSHH1349 pKa = 6.8 LMTTYY1354 pKa = 10.95 GSALQSVMLVDD1365 pKa = 4.91 FDD1367 pKa = 5.09 DD1368 pKa = 3.98 NARR1371 pKa = 11.84 VFHH1374 pKa = 6.53 VNTDD1378 pKa = 3.28 GTGSVWLTQSQAEE1391 pKa = 4.36 SKK1393 pKa = 10.49 YY1394 pKa = 11.2 SSITSGGNTDD1404 pKa = 3.39 YY1405 pKa = 11.71 DD1406 pKa = 3.94 DD1407 pKa = 5.58 AIKK1410 pKa = 10.43 AVQDD1414 pKa = 3.58 NYY1416 pKa = 9.93 GTPPTAAKK1424 pKa = 9.0 TYY1426 pKa = 10.52 VYY1428 pKa = 9.72 FVSDD1432 pKa = 4.2 GEE1434 pKa = 4.41 PTGSDD1439 pKa = 2.92 GTNPNTIEE1447 pKa = 4.0 PSEE1450 pKa = 4.02 RR1451 pKa = 11.84 ADD1453 pKa = 2.88 WVNFLTGKK1461 pKa = 9.72 VDD1463 pKa = 3.32 EE1464 pKa = 5.21 VYY1466 pKa = 10.77 AIGIGSGVSQTDD1478 pKa = 3.0 SDD1480 pKa = 4.51 LLDD1483 pKa = 3.87 VAWSSSGNDD1492 pKa = 3.17 AGNVKK1497 pKa = 10.41 LISTADD1503 pKa = 3.88 DD1504 pKa = 3.72 LSGTLTSIAQSNGNVTSNDD1523 pKa = 3.35 DD1524 pKa = 3.63 FGLDD1528 pKa = 3.56 GAGSPKK1534 pKa = 10.19 LVSVSYY1540 pKa = 10.73 DD1541 pKa = 3.26 ADD1543 pKa = 3.72 GSGPGAATVYY1553 pKa = 10.33 TFDD1556 pKa = 4.13 ATHH1559 pKa = 6.46 HH1560 pKa = 6.89 AYY1562 pKa = 9.39 TINLGAGRR1570 pKa = 11.84 GTLVLHH1576 pKa = 7.61 DD1577 pKa = 5.29 DD1578 pKa = 3.63 GSYY1581 pKa = 10.19 TYY1583 pKa = 9.75 TPASGSSNGAPFTVQYY1599 pKa = 9.46 TIQDD1603 pKa = 3.52 ADD1605 pKa = 3.96 GSQSTASMLFDD1616 pKa = 4.66 PNGTPVVGTAPSITVSEE1633 pKa = 4.32 EE1634 pKa = 3.85 GLPGANPDD1642 pKa = 3.57 TTGNTDD1648 pKa = 3.34 TTNLALRR1655 pKa = 11.84 SGTIAITDD1663 pKa = 3.82 PDD1665 pKa = 3.81 STDD1668 pKa = 3.38 FNLRR1672 pKa = 11.84 FAAPTATLTSGGTALSWSGAGTGVLIGMANGVEE1705 pKa = 4.81 IIRR1708 pKa = 11.84 ISQSDD1713 pKa = 3.41 THH1715 pKa = 7.53 GGYY1718 pKa = 9.33 QVQLSGSLDD1727 pKa = 3.42 HH1728 pKa = 6.91 ATAGQEE1734 pKa = 4.09 DD1735 pKa = 4.61 VKK1737 pKa = 11.41 SFGVLVTVDD1746 pKa = 4.48 DD1747 pKa = 4.5 GAAANNTTTTTLTVNVEE1764 pKa = 4.11 DD1765 pKa = 5.19 DD1766 pKa = 3.92 SPINMITNGLAQNTTSTSFEE1786 pKa = 3.92 GTLASIGADD1795 pKa = 2.79 KK1796 pKa = 10.69 TGAHH1800 pKa = 6.15 IVLSGSVPSGLTSNGVAITYY1820 pKa = 9.82 SLSSDD1825 pKa = 3.26 GSTLTAKK1832 pKa = 10.71 AGTNTVFTLTGHH1844 pKa = 7.23 ADD1846 pKa = 3.02 GSYY1849 pKa = 10.65 SYY1851 pKa = 11.28 DD1852 pKa = 3.25 QVRR1855 pKa = 11.84 TLDD1858 pKa = 3.76 LSVVTSNLQSSVGAGGPQAAYY1879 pKa = 11.2 YY1880 pKa = 9.06 MYY1882 pKa = 10.08 TDD1884 pKa = 4.02 GSFGSTEE1891 pKa = 3.95 NAKK1894 pKa = 10.44 DD1895 pKa = 3.25 WAVKK1899 pKa = 8.75 ITGSDD1904 pKa = 3.74 SINPSTQGMGVSNNLFQTGEE1924 pKa = 3.97 TMRR1927 pKa = 11.84 FEE1929 pKa = 5.0 FDD1931 pKa = 4.14 DD1932 pKa = 3.74 EE1933 pKa = 4.4 HH1934 pKa = 8.05 ASTVGGSTPNLAYY1947 pKa = 8.89 ATKK1950 pKa = 10.32 IGVTDD1955 pKa = 3.75 LTGSEE1960 pKa = 4.14 SLAWTAYY1967 pKa = 8.54 FTDD1970 pKa = 4.8 GSHH1973 pKa = 7.14 ASGVVTAGSLVSGAFTITSPNGLYY1997 pKa = 10.38 LDD1999 pKa = 4.36 YY2000 pKa = 11.51 VDD2002 pKa = 5.43 LAPGSSTSIRR2012 pKa = 11.84 LTSVTNYY2019 pKa = 10.35 VLDD2022 pKa = 4.1 DD2023 pKa = 3.68 THH2025 pKa = 7.11 TKK2027 pKa = 10.56 DD2028 pKa = 4.23 LNFGFTAYY2036 pKa = 10.43 DD2037 pKa = 3.65 GDD2039 pKa = 4.28 GDD2041 pKa = 4.08 TSNGSLTITSQNSATLTGDD2060 pKa = 3.32 ATHH2063 pKa = 6.85 TALGGGNANNTLLGTAGNDD2082 pKa = 2.95 ILTGGAGNDD2091 pKa = 3.65 TLTGGLGADD2100 pKa = 3.53 VFRR2103 pKa = 11.84 WGVNDD2108 pKa = 4.11 HH2109 pKa = 5.97 GTAATPSNDD2118 pKa = 2.9 VVTDD2122 pKa = 4.05 FATTGASHH2130 pKa = 7.28 DD2131 pKa = 3.9 QLDD2134 pKa = 4.28 LRR2136 pKa = 11.84 DD2137 pKa = 4.51 LLTSVNWTVNAGHH2150 pKa = 6.22 TTAQLATEE2158 pKa = 4.33 LDD2160 pKa = 4.25 GYY2162 pKa = 11.21 LSVSEE2167 pKa = 4.72 SGTTTTLSVKK2177 pKa = 10.28 AGSSSVTEE2185 pKa = 4.6 TIQLQNVTDD2194 pKa = 4.33 LVDD2197 pKa = 3.72 GTHH2200 pKa = 5.8 TVSHH2204 pKa = 6.51 DD2205 pKa = 3.74 VIVYY2209 pKa = 9.14 MLDD2212 pKa = 3.2 HH2213 pKa = 7.4 DD2214 pKa = 5.68 LIKK2217 pKa = 10.61 HH2218 pKa = 5.95 DD2219 pKa = 3.93
Molecular weight: 226.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.67
IPC2_protein 3.694
IPC_protein 3.757
Toseland 3.516
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.177
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.884
Patrickios 1.456
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|A0A1W1XU88|A0A1W1XU88_9NEIS Membrane fusion protein multidrug efflux system OS=Andreprevotia lacus DSM 23236 OX=1121001 GN=SAMN02745857_02897 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.58 RR14 pKa = 11.84 THH16 pKa = 6.01 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.35 GRR39 pKa = 11.84 ASLSAA44 pKa = 3.83
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4303
0
4303
1369701
39
7105
318.3
34.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.499 ± 0.053
0.933 ± 0.012
5.516 ± 0.03
4.964 ± 0.04
3.454 ± 0.024
7.954 ± 0.036
2.335 ± 0.021
4.531 ± 0.03
3.387 ± 0.034
11.323 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.162 ± 0.02
3.04 ± 0.028
5.117 ± 0.034
4.651 ± 0.028
6.201 ± 0.042
5.345 ± 0.035
5.289 ± 0.052
7.053 ± 0.033
1.571 ± 0.02
2.675 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here