Gemmobacter aquatilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Gemmobacter

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3845 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8DZF7|A0A1H8DZF7_9RHOB Peptide methionine sulfoxide reductase MsrA OS=Gemmobacter aquatilis OX=933059 GN=msrA PE=3 SV=1
MM1 pKa = 7.4CTLCSALRR9 pKa = 11.84PDD11 pKa = 4.66DD12 pKa = 4.21LAAASDD18 pKa = 3.82LHH20 pKa = 6.32TGAGTGAALPVYY32 pKa = 10.4SLGQIARR39 pKa = 11.84QLTEE43 pKa = 4.97GYY45 pKa = 8.25WAYY48 pKa = 9.87TGRR51 pKa = 11.84DD52 pKa = 3.01MRR54 pKa = 11.84AFAVEE59 pKa = 4.08NGDD62 pKa = 3.73TLTVDD67 pKa = 5.01FGPLSQAEE75 pKa = 4.17RR76 pKa = 11.84AIATAALQAWTDD88 pKa = 3.33VTGITFTAYY97 pKa = 9.56TGDD100 pKa = 4.14DD101 pKa = 3.51PTVIAEE107 pKa = 4.36TTDD110 pKa = 3.3AATGTATTATLPVNGVFSGEE130 pKa = 3.77IDD132 pKa = 3.59STGDD136 pKa = 3.38ADD138 pKa = 4.22WIKK141 pKa = 9.95VTLQKK146 pKa = 10.89GQTYY150 pKa = 10.5RR151 pKa = 11.84ILLEE155 pKa = 4.14SAEE158 pKa = 4.53DD159 pKa = 3.8ADD161 pKa = 5.15ALADD165 pKa = 4.03PLLALHH171 pKa = 7.19DD172 pKa = 3.74ATGAVIATSDD182 pKa = 3.39DD183 pKa = 3.58RR184 pKa = 11.84SRR186 pKa = 11.84GDD188 pKa = 3.52LNSFLSFTAAEE199 pKa = 4.05TGTYY203 pKa = 9.83YY204 pKa = 11.13LSAEE208 pKa = 4.36SATSGSGTYY217 pKa = 11.03YY218 pKa = 9.9MGLVTEE224 pKa = 4.95AAHH227 pKa = 6.41GADD230 pKa = 2.66ISFDD234 pKa = 3.71NRR236 pKa = 11.84DD237 pKa = 3.47SGAYY241 pKa = 9.78AYY243 pKa = 11.01SDD245 pKa = 3.55VAGTRR250 pKa = 11.84ILYY253 pKa = 10.25SYY255 pKa = 11.39VNIARR260 pKa = 11.84NWDD263 pKa = 3.58ASDD266 pKa = 4.75LSLNSYY272 pKa = 8.21WLQTYY277 pKa = 8.66IHH279 pKa = 7.25EE280 pKa = 4.73IGHH283 pKa = 6.42ALGLGHH289 pKa = 7.61AGNYY293 pKa = 6.88NTTASFPTDD302 pKa = 2.54AHH304 pKa = 6.01YY305 pKa = 11.59ANDD308 pKa = 3.71SVTNSIMSYY317 pKa = 9.84FFQGEE322 pKa = 4.25NPNIEE327 pKa = 4.1ADD329 pKa = 3.86FGFLATVMPADD340 pKa = 3.4ILAIQDD346 pKa = 4.08LYY348 pKa = 11.57GSDD351 pKa = 3.65VTTHH355 pKa = 6.96AGDD358 pKa = 3.47TTYY361 pKa = 11.3GANSNVDD368 pKa = 3.47GYY370 pKa = 10.66LGAMFAQIFAEE381 pKa = 4.65APADD385 pKa = 3.42PAVYY389 pKa = 9.91QGEE392 pKa = 4.37NMVLTLYY399 pKa = 9.19DD400 pKa = 3.53TGGHH404 pKa = 6.88DD405 pKa = 6.07LLDD408 pKa = 4.44LSPSRR413 pKa = 11.84GPQVISLLAEE423 pKa = 4.16TFSSIGGYY431 pKa = 10.18LSNLTIARR439 pKa = 11.84GTVIEE444 pKa = 4.68DD445 pKa = 3.66VIAGAGADD453 pKa = 3.59TVLGNAAANMLDD465 pKa = 3.62GAAGSDD471 pKa = 3.49WLIGGAGADD480 pKa = 3.75TLAGGLGNDD489 pKa = 4.25LLKK492 pKa = 11.18GGAGRR497 pKa = 11.84DD498 pKa = 3.43TASFATATRR507 pKa = 11.84GVIVDD512 pKa = 4.63LALTTAQSTGAGQDD526 pKa = 2.85RR527 pKa = 11.84LTGVEE532 pKa = 4.36NLTGSAFNDD541 pKa = 3.6GLGGNGAANVLDD553 pKa = 4.21GVAGRR558 pKa = 11.84DD559 pKa = 3.54LLVGRR564 pKa = 11.84GGNDD568 pKa = 2.88WLLGGAGADD577 pKa = 3.7TLSGGLGRR585 pKa = 11.84DD586 pKa = 3.05AFVFAAGADD595 pKa = 3.66VVLDD599 pKa = 3.82FTDD602 pKa = 5.56DD603 pKa = 3.51EE604 pKa = 5.03DD605 pKa = 4.68SLVFSTALFPGGTTTAEE622 pKa = 3.86EE623 pKa = 4.25LLARR627 pKa = 11.84AAVVSGDD634 pKa = 3.75TVFTFAPGIRR644 pKa = 11.84LILSGFTDD652 pKa = 3.76IAALADD658 pKa = 4.15DD659 pKa = 4.99LLLII663 pKa = 4.93

Molecular weight: 68.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7ZMX2|A0A1H7ZMX2_9RHOB Thiamine-binding periplasmic protein OS=Gemmobacter aquatilis OX=933059 GN=SAMN04488103_101572 PE=3 SV=1
MM1 pKa = 7.32TRR3 pKa = 11.84FLLILIIGAIAGFLATRR20 pKa = 11.84FMRR23 pKa = 11.84VQTSVGVTVLIGMAGASIAWFVLRR47 pKa = 11.84FVMLLTRR54 pKa = 11.84SGFLLVGAVLGAMALIWLWKK74 pKa = 9.14TFLNRR79 pKa = 3.52

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3845

0

3845

1190717

39

2131

309.7

33.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.69 ± 0.063

0.901 ± 0.013

5.634 ± 0.034

5.485 ± 0.03

3.601 ± 0.023

9.049 ± 0.042

2.056 ± 0.02

4.805 ± 0.029

2.96 ± 0.033

10.632 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.018

2.306 ± 0.026

5.398 ± 0.04

3.187 ± 0.02

6.942 ± 0.043

4.616 ± 0.025

5.322 ± 0.024

7.26 ± 0.033

1.405 ± 0.016

2.083 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski