Whitefly-associated begomovirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0J7I8|A0A0P0J7I8_9GEMI Replication-associated protein OS=Whitefly-associated begomovirus 3 OX=2169741 PE=3 SV=1
MM1 pKa = 7.89PPPKK5 pKa = 9.89KK6 pKa = 9.54WNAKK10 pKa = 10.14NYY12 pKa = 9.32FLTYY16 pKa = 9.3SQCSLTKK23 pKa = 10.67EE24 pKa = 4.02EE25 pKa = 5.4ALSQLLNLNTPTNKK39 pKa = 9.88KK40 pKa = 9.69FIKK43 pKa = 9.67ICRR46 pKa = 11.84EE47 pKa = 3.75LHH49 pKa = 6.53QDD51 pKa = 3.81GQPHH55 pKa = 6.38LHH57 pKa = 6.21VLIQFEE63 pKa = 4.77GRR65 pKa = 11.84YY66 pKa = 8.98QCANKK71 pKa = 10.24RR72 pKa = 11.84FFDD75 pKa = 3.81LVSPNRR81 pKa = 11.84SKK83 pKa = 11.12LFHH86 pKa = 6.9PNIQGARR93 pKa = 11.84SSSDD97 pKa = 2.93VKK99 pKa = 11.21SYY101 pKa = 10.85VEE103 pKa = 4.33KK104 pKa = 11.05EE105 pKa = 3.51GDD107 pKa = 3.59TIEE110 pKa = 4.08WGLFQVDD117 pKa = 3.18GRR119 pKa = 11.84SARR122 pKa = 11.84GGQQTANDD130 pKa = 3.91AAAEE134 pKa = 3.97ALNAGTKK141 pKa = 9.59EE142 pKa = 3.8EE143 pKa = 3.78AMKK146 pKa = 10.24IIKK149 pKa = 9.78EE150 pKa = 3.92KK151 pKa = 10.82LPEE154 pKa = 4.29KK155 pKa = 10.62FLFQYY160 pKa = 11.01HH161 pKa = 6.01NLSSNLDD168 pKa = 3.94RR169 pKa = 11.84IFSKK173 pKa = 10.88APEE176 pKa = 3.92QWVPPFPLSSFTNVPDD192 pKa = 4.94EE193 pKa = 4.03MQEE196 pKa = 3.55WSDD199 pKa = 3.61AYY201 pKa = 10.54FGRR204 pKa = 11.84GSAARR209 pKa = 11.84PEE211 pKa = 4.36RR212 pKa = 11.84PVSLIVEE219 pKa = 4.72GDD221 pKa = 3.34SRR223 pKa = 11.84TGKK226 pKa = 8.55TMWARR231 pKa = 11.84ALGPHH236 pKa = 6.63NYY238 pKa = 10.14LSGHH242 pKa = 6.84LDD244 pKa = 3.54FNARR248 pKa = 11.84VYY250 pKa = 11.48SNLVEE255 pKa = 4.22YY256 pKa = 10.94NVIDD260 pKa = 4.41DD261 pKa = 4.14VAPHH265 pKa = 5.29YY266 pKa = 10.97LKK268 pKa = 10.78LKK270 pKa = 9.14HH271 pKa = 5.83WKK273 pKa = 9.52EE274 pKa = 3.61LLGAQKK280 pKa = 10.6DD281 pKa = 3.68WQSNCKK287 pKa = 9.73YY288 pKa = 10.21GRR290 pKa = 11.84PVQIKK295 pKa = 9.96GGIPSIVLCNPGEE308 pKa = 4.16GASYY312 pKa = 11.26KK313 pKa = 10.85DD314 pKa = 3.5FLEE317 pKa = 5.15KK318 pKa = 10.92EE319 pKa = 4.33EE320 pKa = 4.07NASLKK325 pKa = 10.67NWTLKK330 pKa = 10.43NAVFITLEE338 pKa = 3.9SPLYY342 pKa = 10.65KK343 pKa = 10.53EE344 pKa = 4.93GTQEE348 pKa = 4.11SQAEE352 pKa = 4.48GNN354 pKa = 3.63

Molecular weight: 40.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0J7I8|A0A0P0J7I8_9GEMI Replication-associated protein OS=Whitefly-associated begomovirus 3 OX=2169741 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.62RR4 pKa = 11.84DD5 pKa = 3.86APWRR9 pKa = 11.84TMAGTSKK16 pKa = 10.54VSRR19 pKa = 11.84NANYY23 pKa = 10.27SPRR26 pKa = 11.84GGSGPKK32 pKa = 9.78VSRR35 pKa = 11.84ASEE38 pKa = 3.92WVNRR42 pKa = 11.84PMYY45 pKa = 9.79RR46 pKa = 11.84KK47 pKa = 8.83PRR49 pKa = 11.84IYY51 pKa = 9.64RR52 pKa = 11.84TMRR55 pKa = 11.84TPDD58 pKa = 3.19VPRR61 pKa = 11.84GCEE64 pKa = 4.34GPCKK68 pKa = 9.32VQSYY72 pKa = 6.94EE73 pKa = 3.74QRR75 pKa = 11.84HH76 pKa = 6.38DD77 pKa = 3.16ISHH80 pKa = 6.13VGKK83 pKa = 10.55VMCISDD89 pKa = 3.56VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.16ILGKK114 pKa = 9.37IWMDD118 pKa = 3.39EE119 pKa = 4.0NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMFDD155 pKa = 3.99NEE157 pKa = 4.11PSTATVKK164 pKa = 10.85NDD166 pKa = 2.82LRR168 pKa = 11.84DD169 pKa = 3.54RR170 pKa = 11.84FQVMHH175 pKa = 6.9KK176 pKa = 10.21FYY178 pKa = 11.22GKK180 pKa = 8.61VTGGQYY186 pKa = 11.07ASNEE190 pKa = 3.62QAIIRR195 pKa = 11.84RR196 pKa = 11.84FWRR199 pKa = 11.84VNNYY203 pKa = 7.91VVYY206 pKa = 10.46NHH208 pKa = 6.03QEE210 pKa = 3.42AAKK213 pKa = 10.4YY214 pKa = 9.25EE215 pKa = 4.13NHH217 pKa = 6.46TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.6FYY246 pKa = 11.36DD247 pKa = 4.19SILNN251 pKa = 3.75

Molecular weight: 29.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

951

85

354

190.2

21.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.783 ± 0.571

1.788 ± 0.452

4.416 ± 0.397

4.942 ± 1.099

3.891 ± 0.464

6.204 ± 0.515

3.049 ± 0.539

4.837 ± 0.477

5.783 ± 1.195

7.256 ± 1.401

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.661

6.309 ± 0.579

5.258 ± 0.87

4.522 ± 0.551

7.571 ± 1.349

8.097 ± 1.223

5.468 ± 0.907

6.099 ± 0.938

1.893 ± 0.183

4.627 ± 0.894
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski