Sulfurovum sp. bin170
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2107 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M0KY29|A0A6M0KY29_9PROT WG repeat-containing protein OS=Sulfurovum sp. bin170 OX=2695268 GN=GSY74_03800 PE=4 SV=1
MM1 pKa = 7.13 KK2 pKa = 10.37 QILTASIISSMIIFSGCGSTEE23 pKa = 3.76 GDD25 pKa = 3.93 DD26 pKa = 3.91 NLGVLDD32 pKa = 4.75 DD33 pKa = 4.84 DD34 pKa = 4.67 KK35 pKa = 11.73 PRR37 pKa = 11.84 ITLTANPDD45 pKa = 3.38 TGSTDD50 pKa = 2.8 IEE52 pKa = 4.39 LRR54 pKa = 11.84 LGDD57 pKa = 3.94 TQIPEE62 pKa = 3.92 YY63 pKa = 10.91 GYY65 pKa = 10.07 IAEE68 pKa = 4.74 DD69 pKa = 3.76 VVDD72 pKa = 4.72 GDD74 pKa = 4.89 LSVDD78 pKa = 3.53 VQRR81 pKa = 11.84 TNDD84 pKa = 3.04 IDD86 pKa = 3.73 LTKK89 pKa = 10.77 VGTYY93 pKa = 9.08 TITYY97 pKa = 9.22 FVEE100 pKa = 4.42 DD101 pKa = 3.69 TDD103 pKa = 5.44 GYY105 pKa = 9.47 TDD107 pKa = 3.26 TKK109 pKa = 10.01 TRR111 pKa = 11.84 TVTIVASNDD120 pKa = 3.3 DD121 pKa = 4.28 YY122 pKa = 12.23 SNGDD126 pKa = 3.48 YY127 pKa = 11.46 SNDD130 pKa = 3.56 EE131 pKa = 4.19 YY132 pKa = 11.73 SDD134 pKa = 4.23 DD135 pKa = 4.2 LSTYY139 pKa = 10.35 NPNAEE144 pKa = 4.09 GNIDD148 pKa = 3.77 LGEE151 pKa = 4.21 QIGSSQGADD160 pKa = 3.18 YY161 pKa = 10.83 GAGIDD166 pKa = 5.71 GFTSWYY172 pKa = 10.63 YY173 pKa = 10.38 DD174 pKa = 3.22 VCNQNFNASLYY185 pKa = 9.55 NAGTGSYY192 pKa = 10.02 NGTITCSNNLNSIDD206 pKa = 4.08 LTPVSIFTTINGLDD220 pKa = 3.64 LSNNNLTYY228 pKa = 10.2 IDD230 pKa = 4.36 FSVLGLNEE238 pKa = 4.14 STNNTKK244 pKa = 9.81 ILKK247 pKa = 10.4 SLDD250 pKa = 3.31 LRR252 pKa = 11.84 TNSLNTQNFDD262 pKa = 3.52 LFRR265 pKa = 11.84 PLHH268 pKa = 5.0 YY269 pKa = 10.81 LKK271 pKa = 10.73 DD272 pKa = 3.4 IDD274 pKa = 4.95 KK275 pKa = 11.13 LYY277 pKa = 10.44 IANNNFNYY285 pKa = 10.06 TCDD288 pKa = 3.73 DD289 pKa = 4.1 LYY291 pKa = 11.57 KK292 pKa = 10.86 LRR294 pKa = 11.84 TDD296 pKa = 4.45 KK297 pKa = 11.2 EE298 pKa = 4.25 LFNNGSLDD306 pKa = 3.2 IDD308 pKa = 3.57 RR309 pKa = 11.84 EE310 pKa = 4.15 RR311 pKa = 11.84 EE312 pKa = 4.06 QNCPSLQNKK321 pKa = 8.44
Molecular weight: 35.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.592
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.656
Grimsley 3.49
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.253
Thurlkill 3.656
EMBOSS 3.808
Sillero 3.961
Patrickios 0.858
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A6M0KWF8|A0A6M0KWF8_9PROT tRNA threonylcarbamoyladenosine dehydratase OS=Sulfurovum sp. bin170 OX=2695268 GN=GSY74_00490 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPHH8 pKa = 4.68 NTPKK12 pKa = 10.51 KK13 pKa = 7.61 RR14 pKa = 11.84 THH16 pKa = 6.28 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MKK23 pKa = 8.87 TKK25 pKa = 10.11 NGRR28 pKa = 11.84 KK29 pKa = 9.33 VISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2107
0
2107
644486
30
2107
305.9
34.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.069 ± 0.056
0.814 ± 0.017
6.063 ± 0.049
7.328 ± 0.055
4.718 ± 0.047
6.134 ± 0.054
1.8 ± 0.021
8.702 ± 0.052
8.215 ± 0.066
9.424 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.617 ± 0.028
5.067 ± 0.041
2.999 ± 0.031
2.864 ± 0.029
4.004 ± 0.038
7.07 ± 0.049
5.264 ± 0.053
6.008 ± 0.042
0.817 ± 0.02
4.024 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here