Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus) (Tritirachium shiotae)
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10363 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J5JI07|J5JI07_BEAB2 Protein transport protein SFT2 OS=Beauveria bassiana (strain ARSEF 2860) OX=655819 GN=BBA_08082 PE=3 SV=1
MM1 pKa = 7.81 ADD3 pKa = 4.0 APGQCTVLYY12 pKa = 9.2 FAAASSYY19 pKa = 9.15 TGKK22 pKa = 10.4 EE23 pKa = 4.03 SEE25 pKa = 4.67 VFSAPTPLARR35 pKa = 11.84 LLAAVEE41 pKa = 3.75 ARR43 pKa = 11.84 YY44 pKa = 9.11 PGIRR48 pKa = 11.84 ASVLDD53 pKa = 3.73 SCLVTVNLDD62 pKa = 3.72 YY63 pKa = 11.71 VDD65 pKa = 4.22 VPSGDD70 pKa = 3.87 DD71 pKa = 3.38 EE72 pKa = 4.79 GVLIQAADD80 pKa = 3.9 EE81 pKa = 4.26 VAIIPPVSSGG91 pKa = 3.12
Molecular weight: 9.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|J4UGG7|J4UGG7_BEAB2 Glyoxalase-like protein OS=Beauveria bassiana (strain ARSEF 2860) OX=655819 GN=BBA_09103 PE=4 SV=1
MM1 pKa = 7.9 LEE3 pKa = 4.07 VTSSCFAPLISSTLLSFGGNLYY25 pKa = 9.78 TPGCFAIQKK34 pKa = 7.74 KK35 pKa = 8.0 TSLLCTPYY43 pKa = 10.27 RR44 pKa = 11.84 CPATQSTPIASMLSAIPEE62 pKa = 4.26 LACRR66 pKa = 11.84 MRR68 pKa = 11.84 SLAQSHH74 pKa = 5.28 GHH76 pKa = 5.7 DD77 pKa = 3.7 VRR79 pKa = 11.84 PEE81 pKa = 3.74 VLTRR85 pKa = 11.84 RR86 pKa = 11.84 QVFITFSDD94 pKa = 3.65 AKK96 pKa = 10.65 SNAPPRR102 pKa = 11.84 VTDD105 pKa = 4.98 KK106 pKa = 10.76 AAAWKK111 pKa = 9.88 RR112 pKa = 11.84 VQRR115 pKa = 11.84 GFACLTRR122 pKa = 11.84 FYY124 pKa = 11.09 RR125 pKa = 11.84 PLCPRR130 pKa = 11.84 RR131 pKa = 11.84 MSFLSSDD138 pKa = 3.45 RR139 pKa = 11.84 SLQRR143 pKa = 11.84 DD144 pKa = 3.95 PEE146 pKa = 4.43 TAQPPLNVARR156 pKa = 11.84 RR157 pKa = 11.84 WGLQFRR163 pKa = 11.84 FACSSQTICRR173 pKa = 11.84 VAAIEE178 pKa = 4.37 KK179 pKa = 9.71 RR180 pKa = 11.84 PSSASARR187 pKa = 11.84 EE188 pKa = 4.18 WTPKK192 pKa = 8.82 LCIWFRR198 pKa = 11.84 CITAIRR204 pKa = 11.84 PRR206 pKa = 11.84 KK207 pKa = 8.85 VPRR210 pKa = 11.84 IHH212 pKa = 6.82 YY213 pKa = 8.08 EE214 pKa = 3.93 LVSAALPWSVSTDD227 pKa = 3.3 SEE229 pKa = 4.31 EE230 pKa = 4.18 LSKK233 pKa = 10.28 KK234 pKa = 7.7 TSHH237 pKa = 5.95 RR238 pKa = 11.84 TSHH241 pKa = 5.75 VAMSMSFRR249 pKa = 11.84 LTSSMQRR256 pKa = 11.84 PSSQAYY262 pKa = 8.4 EE263 pKa = 4.19 VLL265 pKa = 3.76
Molecular weight: 29.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.458
IPC_protein 10.028
Toseland 10.438
ProMoST 10.116
Dawson 10.57
Bjellqvist 10.292
Wikipedia 10.76
Rodwell 10.76
Grimsley 10.613
Solomon 10.643
Lehninger 10.613
Nozaki 10.511
DTASelect 10.262
Thurlkill 10.452
EMBOSS 10.833
Sillero 10.511
Patrickios 10.452
IPC_peptide 10.657
IPC2_peptide 9.692
IPC2.peptide.svr19 8.445
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10363
0
10363
5172183
50
5124
499.1
55.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.62 ± 0.023
1.288 ± 0.008
5.883 ± 0.018
5.887 ± 0.022
3.62 ± 0.014
6.907 ± 0.021
2.44 ± 0.011
4.644 ± 0.015
4.69 ± 0.021
8.889 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.234 ± 0.009
3.508 ± 0.012
5.837 ± 0.02
4.082 ± 0.019
6.271 ± 0.02
8.035 ± 0.025
5.888 ± 0.015
6.177 ± 0.018
1.439 ± 0.008
2.66 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here