Celeribacter baekdonensis B30
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4198 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K2JW45|K2JW45_9RHOB Protoporphyrinogen IX oxidase OS=Celeribacter baekdonensis B30 OX=1208323 GN=B30_02380 PE=3 SV=1
MM1 pKa = 6.64 TNYY4 pKa = 10.58 YY5 pKa = 9.48 IVSSNPEE12 pKa = 3.57 VHH14 pKa = 7.96 DD15 pKa = 4.81 DD16 pKa = 4.05 GNVNNYY22 pKa = 7.25 TQVQAGALTPQEE34 pKa = 3.55 GDD36 pKa = 3.0 AYY38 pKa = 11.03 YY39 pKa = 11.27 LDD41 pKa = 5.06 GNLSSSLWFYY51 pKa = 11.32 GSASFTVNIEE61 pKa = 3.82 QSLVTNNLVGGATMWIQGTTNATVNIGAGVDD92 pKa = 3.32 ADD94 pKa = 3.98 YY95 pKa = 11.8 LNISSSGDD103 pKa = 3.19 TLTINVDD110 pKa = 3.49 DD111 pKa = 4.88 GATLGAITAPLTDD124 pKa = 3.11 QMTIVAGNDD133 pKa = 2.95 VTMNGALIGSNGDD146 pKa = 3.41 STVTFGSGATFNSTVNFDD164 pKa = 3.09 GWGTLSEE171 pKa = 4.41 TGSQTFSVGDD181 pKa = 3.53 NAVFEE186 pKa = 4.97 YY187 pKa = 10.2 GVSMDD192 pKa = 3.76 GSYY195 pKa = 8.45 THH197 pKa = 6.86 NSFSAGVNSTIGDD210 pKa = 4.31 DD211 pKa = 3.18 IVMNGSGYY219 pKa = 10.39 INLTDD224 pKa = 3.74 DD225 pKa = 3.28 TSGYY229 pKa = 9.46 SGSVTVGDD237 pKa = 4.04 GSTVQGNVYY246 pKa = 9.92 MGGSYY251 pKa = 11.49 GDD253 pKa = 3.31 MTVAVGNDD261 pKa = 3.26 VSVGDD266 pKa = 3.88 IAVGGSFNDD275 pKa = 3.03 IDD277 pKa = 3.67 ITVGDD282 pKa = 4.36 RR283 pKa = 11.84 VDD285 pKa = 2.93 TGYY288 pKa = 10.6 IGMGGSDD295 pKa = 3.13 INGRR299 pKa = 11.84 ILVGSEE305 pKa = 4.06 GNVSAIYY312 pKa = 10.44 AGGSSSDD319 pKa = 3.0 ICVGVGDD326 pKa = 4.36 NTNIDD331 pKa = 3.58 LSIAMGGSNDD341 pKa = 3.73 SYY343 pKa = 11.66 ALSLGDD349 pKa = 3.78 GVSIGLDD356 pKa = 3.19 LSMGGSNNVQNVVMGDD372 pKa = 3.66 DD373 pKa = 3.87 VSVGTYY379 pKa = 10.28 VFMGGVDD386 pKa = 3.53 NTNHH390 pKa = 5.75 VEE392 pKa = 3.64 IGNNFTLGSHH402 pKa = 4.9 WTGSISGSDD411 pKa = 3.51 YY412 pKa = 11.66 LEE414 pKa = 5.8 IGDD417 pKa = 4.09 NWDD420 pKa = 3.39 IGTGIYY426 pKa = 10.08 LQGGDD431 pKa = 3.9 DD432 pKa = 3.9 TLILGTVADD441 pKa = 4.85 GVTAYY446 pKa = 9.78 IDD448 pKa = 3.81 GGLGTDD454 pKa = 3.3 ALSLRR459 pKa = 11.84 VAAGDD464 pKa = 3.52 TGFVSAATEE473 pKa = 4.16 AGWTYY478 pKa = 11.55 DD479 pKa = 3.28 SDD481 pKa = 3.72 TGTWSSNGNDD491 pKa = 3.2 LTYY494 pKa = 11.28 NGVTYY499 pKa = 10.41 TSFEE503 pKa = 3.96 TAAVVDD509 pKa = 4.33 EE510 pKa = 4.71 DD511 pKa = 4.12 WEE513 pKa = 4.61 LVCPVANNIVDD524 pKa = 4.09 GTAGDD529 pKa = 4.03 DD530 pKa = 3.85 VMDD533 pKa = 4.46 IGYY536 pKa = 8.6 TDD538 pKa = 3.6 AQGDD542 pKa = 4.13 QIDD545 pKa = 4.4 GTDD548 pKa = 3.54 GDD550 pKa = 3.98 TDD552 pKa = 4.19 VIFGYY557 pKa = 10.87 AGDD560 pKa = 3.82 DD561 pKa = 4.1 TINGGAGNDD570 pKa = 4.18 TIHH573 pKa = 7.12 GGLDD577 pKa = 3.18 NDD579 pKa = 5.21 TITGGAGDD587 pKa = 4.4 DD588 pKa = 4.2 SVTGGAGSDD597 pKa = 3.49 TLAGGVGADD606 pKa = 3.79 VLDD609 pKa = 4.68 GGSGDD614 pKa = 4.31 DD615 pKa = 4.22 SLDD618 pKa = 3.52 GGEE621 pKa = 5.42 GDD623 pKa = 5.57 DD624 pKa = 4.49 EE625 pKa = 4.84 LLGGLGADD633 pKa = 3.91 TLIGGAGADD642 pKa = 3.84 TLTGGDD648 pKa = 3.76 GADD651 pKa = 4.11 LFIVDD656 pKa = 5.31 DD657 pKa = 4.81 GGDD660 pKa = 3.69 TITDD664 pKa = 4.14 FDD666 pKa = 4.06 ATSGIGDD673 pKa = 4.73 DD674 pKa = 5.11 DD675 pKa = 4.82 EE676 pKa = 6.91 SDD678 pKa = 3.59 NDD680 pKa = 3.94 VVDD683 pKa = 5.26 LSTFYY688 pKa = 11.48 NEE690 pKa = 3.75 TTLAEE695 pKa = 4.02 WNAANPTQTYY705 pKa = 10.14 ASPLGWLRR713 pKa = 11.84 ADD715 pKa = 3.53 QGDD718 pKa = 4.12 GVLDD722 pKa = 3.85 AASGLEE728 pKa = 4.32 LYY730 pKa = 10.83 SPDD733 pKa = 3.63 GNPVTSADD741 pKa = 3.69 LNAEE745 pKa = 4.06 NTRR748 pKa = 11.84 VICFARR754 pKa = 11.84 GTRR757 pKa = 11.84 IMTPRR762 pKa = 11.84 GEE764 pKa = 4.62 VEE766 pKa = 3.79 IQNLKK771 pKa = 10.67 VGDD774 pKa = 3.96 NVLTLDD780 pKa = 4.45 NGFKK784 pKa = 9.46 PIRR787 pKa = 11.84 WIGHH791 pKa = 5.4 RR792 pKa = 11.84 RR793 pKa = 11.84 LGAEE797 pKa = 4.92 ALDD800 pKa = 4.07 AMPKK804 pKa = 9.89 LRR806 pKa = 11.84 PIRR809 pKa = 11.84 IRR811 pKa = 11.84 AGALDD816 pKa = 3.55 TDD818 pKa = 4.17 LPVRR822 pKa = 11.84 DD823 pKa = 4.24 LVVSPQHH830 pKa = 6.14 RR831 pKa = 11.84 VLFRR835 pKa = 11.84 SPHH838 pKa = 5.63 AEE840 pKa = 3.85 LMFGTHH846 pKa = 5.62 EE847 pKa = 4.16 VLLAARR853 pKa = 11.84 QLLGLEE859 pKa = 4.75 GVCVEE864 pKa = 4.45 RR865 pKa = 11.84 EE866 pKa = 4.09 ARR868 pKa = 11.84 EE869 pKa = 3.72 VEE871 pKa = 4.15 YY872 pKa = 10.08 WHH874 pKa = 6.74 FLFNRR879 pKa = 11.84 HH880 pKa = 5.98 EE881 pKa = 4.09 IVFAEE886 pKa = 5.04 GAASEE891 pKa = 4.19 SLYY894 pKa = 10.92 TGVEE898 pKa = 4.03 ALKK901 pKa = 10.77 SLNPEE906 pKa = 3.33 ARR908 pKa = 11.84 EE909 pKa = 3.91 EE910 pKa = 4.0 IFALFPEE917 pKa = 5.14 LGADD921 pKa = 3.39 EE922 pKa = 4.52 ATRR925 pKa = 11.84 KK926 pKa = 9.46 PDD928 pKa = 3.34 LARR931 pKa = 11.84 PVVNGVVARR940 pKa = 11.84 LLVSVNGSGQTANILHH956 pKa = 6.7
Molecular weight: 98.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.541
IPC_protein 3.617
Toseland 3.363
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.579
Rodwell 3.427
Grimsley 3.274
Solomon 3.617
Lehninger 3.567
Nozaki 3.719
DTASelect 4.024
Thurlkill 3.427
EMBOSS 3.579
Sillero 3.732
Patrickios 1.138
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.693
Protein with the highest isoelectric point:
>tr|K2JWU2|K2JWU2_9RHOB Uncharacterized protein OS=Celeribacter baekdonensis B30 OX=1208323 GN=B30_03430 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 7.9 PTPRR6 pKa = 11.84 DD7 pKa = 3.25 LVITRR12 pKa = 11.84 WGARR16 pKa = 11.84 FMGRR20 pKa = 11.84 RR21 pKa = 11.84 FPCSIGKK28 pKa = 10.06 GGIVANKK35 pKa = 9.98 RR36 pKa = 11.84 EE37 pKa = 4.36 GDD39 pKa = 3.41 GGTPIGPHH47 pKa = 6.85 EE48 pKa = 4.25 IAEE51 pKa = 4.3 LYY53 pKa = 10.12 YY54 pKa = 10.78 RR55 pKa = 11.84 ADD57 pKa = 3.69 RR58 pKa = 11.84 VPALNGTEE66 pKa = 3.82 IRR68 pKa = 11.84 IGDD71 pKa = 4.58 LWCDD75 pKa = 3.37 ATDD78 pKa = 3.51 HH79 pKa = 6.53 PRR81 pKa = 11.84 YY82 pKa = 9.11 NHH84 pKa = 6.91 LVRR87 pKa = 11.84 APFKK91 pKa = 10.69 ASHH94 pKa = 5.66 EE95 pKa = 4.14 KK96 pKa = 9.25 MRR98 pKa = 11.84 RR99 pKa = 11.84 GDD101 pKa = 3.38 RR102 pKa = 11.84 LYY104 pKa = 11.38 DD105 pKa = 4.11 LVLTTNWNWPEE116 pKa = 3.75 ATPGKK121 pKa = 10.34 GSAIFIHH128 pKa = 6.76 RR129 pKa = 11.84 WRR131 pKa = 11.84 KK132 pKa = 8.14 PRR134 pKa = 11.84 HH135 pKa = 4.52 PTEE138 pKa = 4.0 GCVAFRR144 pKa = 11.84 ADD146 pKa = 3.42 HH147 pKa = 6.11 LRR149 pKa = 11.84 WIAEE153 pKa = 4.3 RR154 pKa = 11.84 ISPATRR160 pKa = 11.84 LIVRR164 pKa = 4.59
Molecular weight: 18.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.502
IPC_protein 10.292
Toseland 10.511
ProMoST 10.409
Dawson 10.628
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.789
Grimsley 10.687
Solomon 10.745
Lehninger 10.701
Nozaki 10.526
DTASelect 10.35
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.496
IPC_peptide 10.745
IPC2_peptide 9.458
IPC2.peptide.svr19 8.679
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4198
0
4198
1288073
19
2756
306.8
33.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.833 ± 0.05
0.891 ± 0.012
6.009 ± 0.032
5.829 ± 0.038
3.867 ± 0.023
8.489 ± 0.039
2.118 ± 0.017
5.431 ± 0.025
3.503 ± 0.029
10.07 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.921 ± 0.016
2.615 ± 0.021
4.962 ± 0.027
3.119 ± 0.02
6.238 ± 0.037
5.437 ± 0.026
5.742 ± 0.028
7.312 ± 0.031
1.334 ± 0.017
2.28 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here