Saprolegnia diclina (strain VS20)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae; Saprolegnia; Saprolegnia diclina

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0PU04|T0PU04_SAPDV PALP domain-containing protein OS=Saprolegnia diclina (strain VS20) OX=1156394 GN=SDRG_13301 PE=3 SV=1
MM1 pKa = 7.9ADD3 pKa = 3.0HH4 pKa = 7.46RR5 pKa = 11.84SNQGHH10 pKa = 7.13DD11 pKa = 3.38FAEE14 pKa = 4.66DD15 pKa = 3.66EE16 pKa = 4.3EE17 pKa = 5.1EE18 pKa = 4.1EE19 pKa = 4.61TISADD24 pKa = 3.2LGGSYY29 pKa = 10.67EE30 pKa = 4.09EE31 pKa = 4.47NKK33 pKa = 10.24LDD35 pKa = 3.55EE36 pKa = 4.69AMGVIQDD43 pKa = 3.84VLIDD47 pKa = 3.77PSFVQMQNDD56 pKa = 3.67FCEE59 pKa = 4.33THH61 pKa = 6.92CDD63 pKa = 3.77CFDD66 pKa = 4.13DD67 pKa = 5.03ASEE70 pKa = 4.14NKK72 pKa = 9.78LIYY75 pKa = 9.28MDD77 pKa = 4.92LFRR80 pKa = 11.84EE81 pKa = 4.12YY82 pKa = 10.96TNLIEE87 pKa = 4.76NFLEE91 pKa = 4.24ARR93 pKa = 11.84LKK95 pKa = 10.55EE96 pKa = 4.6AIDD99 pKa = 4.32DD100 pKa = 4.12FSMDD104 pKa = 4.43ALCSMIQEE112 pKa = 5.13HH113 pKa = 6.61EE114 pKa = 4.37DD115 pKa = 3.58EE116 pKa = 4.42MTGDD120 pKa = 3.91VIDD123 pKa = 4.48VLMSCADD130 pKa = 3.32FDD132 pKa = 4.18EE133 pKa = 5.66FKK135 pKa = 11.37SLMLSYY141 pKa = 10.46KK142 pKa = 9.84SHH144 pKa = 6.2QATLFQIHH152 pKa = 7.08GDD154 pKa = 3.87SLVCGG159 pKa = 3.93

Molecular weight: 18.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0PUZ6|T0PUZ6_SAPDV Nudix hydrolase domain-containing protein OS=Saprolegnia diclina (strain VS20) OX=1156394 GN=SDRG_13008 PE=4 SV=1
MM1 pKa = 7.22WQLQRR6 pKa = 11.84WATCFLALGLCMAVLGGTGRR26 pKa = 11.84SSKK29 pKa = 9.22TCACALQCQCLGARR43 pKa = 11.84KK44 pKa = 9.89RR45 pKa = 11.84MVDD48 pKa = 3.42GFLTPLVKK56 pKa = 10.28RR57 pKa = 11.84KK58 pKa = 10.2SPSVPVNVVQEE69 pKa = 4.46LYY71 pKa = 10.66DD72 pKa = 3.66VRR74 pKa = 11.84HH75 pKa = 6.26WDD77 pKa = 3.49AATCAPTRR85 pKa = 11.84NDD87 pKa = 2.98VGDD90 pKa = 3.81FFGLLWKK97 pKa = 9.24TPTEE101 pKa = 4.25MPTSRR106 pKa = 11.84WLTKK110 pKa = 9.82PRR112 pKa = 11.84LYY114 pKa = 9.71WRR116 pKa = 11.84SRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84SRR122 pKa = 11.84SAMPFAGWTRR132 pKa = 3.27

Molecular weight: 15.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17306

741

18047

8784450

34

12825

486.8

53.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.906 ± 0.026

1.818 ± 0.009

5.784 ± 0.018

4.985 ± 0.019

3.583 ± 0.013

5.541 ± 0.021

2.914 ± 0.012

3.902 ± 0.012

4.187 ± 0.02

10.612 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.008

2.989 ± 0.011

5.437 ± 0.017

3.725 ± 0.012

5.87 ± 0.018

7.248 ± 0.016

6.482 ± 0.019

7.5 ± 0.017

1.334 ± 0.007

2.75 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski